Data/hora: 20/06/2024 03:15:26

Biblioteca(s): 	Embrapa Semiárido.	
Data corrente: 	20/08/2018	
Data da última atualização: 	22/08/2018	
Autoria: 	NUNES, G. F. de O.; MENEZES, K. A. S.; SAMPAIO, A. A.; LEITE, J.; FERNANDES JUNIOR, P. I.; SEIDO, S. L.; ZILLI, J. E.; MARTINS, L. M. V.	
Afiliação: 	GERSIKA FAKIRRA DE OLIVEIRA NUNES, UNEB; KELLY ALEXSANDRA SOUZA MENEZES, UFAL; ALINE ARAÚJO SAMPAIO, UFBA; JAKSON LEITE, UFAL; PAULO IVAN FERNANDES JUNIOR, CPATSA; SIRANDO LIMA SEIDO, UFRPE; JERRI EDSON ZILLI, CNPAB; LINDETE MÍRIA VIEIRA MARTINS, UNEB.	
Título: 	Polyphasic characterization of forage legumes root nodule bacteria isolated from semiarid region in Brazil.	
Ano de publicação: 	2018	
Fonte/Imprenta: 	Revista de Ciências Agrárias, Lisboa, v. 41, n. 3, p. 612-624, jul./set. 2018.	
DOI: 	http://dx.doi.org/10.19084/RCA17339	
Idioma: 	Português	
Conteúdo: 	Forage legumes are important resources in semiarid regions due to their abilities to adapt to soils with low fertility and fix nitrogen when associated with diazotrophic bacteria. Here, we applied a polyphasic approach to characterize a set of legume nodule isolates obtained from Clitoria ternatea and Stylosllntlzes capitata cultivated in the soils of a semiarid region of Brazil. A tolal of 126 bacterial isolates were obtained: 45 isolates frorn C. ternatea and 81 isolates from S. capitata. Nodulation tests revealed only ten isolates that nodulated their original host: six isolates from C. iernaiea and four isolates from S. capitata. These ten legume nodule isolates were phenotypically and genotypically characterized. All isolates grew in fructose, glucose, sodium glutamate, maltose, xylose. and sucrose. Metabolic tests showed a relationship between tolerance to salt and high temperaturas, where isolates that tolerated the highest salt concentration also tolerated the highest ternperature. Three isolates showed amylolytic activity, and four isolates showed carboxymethyl cellulolytic activity. 5treptomycin was the most limiting and nalidixic acid was the least limiting antibiotic to bacterial growth. Seven out of ten isolates were indol-acetic acid producers. Additionally, 165 rRNA gene partial sequencing enabled the identification of members of the genera Bacillus (1), Bradvrhizobium (4), Leifeonia (3), Microvirga (1), and Rhizobium (1). These data reveal phenotypically and genotypically di verse bacteria inhabiting the nodules of the forage legumes C. tematea and S. capitata represent an important microbial source to protect new biotechnological products and improve forage legumes in semiarid regions.	
Palavras-Chave: 	Fixação biológica do nitrogênio.	
Thesagro: 	Clitoria Ternatea; Controle Biológico; Fixação de Nitrogênio; Leguminosa Forrageira.	
Thesaurus Nal: 	Biological control; Bradyrhizobium; Forage legumes; Nitrogen fixation; Stylosanthes capitata.	
Categoria do assunto: 	F Plantas e Produtos de Origem Vegetal	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/181656/1/RCA-vol41-n3-art04-RCA17339.pdf	
Registro original: 	Embrapa Semiárido (CPATSA)	
Exemplares: 	CPATSA - (57467-1) - UPC - AP - DD -  -  -  - DIS