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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
26/09/2016 |
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Data da última atualização: |
26/10/2016 |
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Autoria: |
HAMILTON, R. P.; SEAMARK, R. F. |
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Título: |
Lipid content of sheep ovarian follicles in culture. |
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Ano de publicação: |
1978 |
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Fonte/Imprenta: |
Australian Journal of Biological Sciences, v. 31, n. 6, p. 621-628, Dec. 1978. |
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Idioma: |
Inglês |
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Conteúdo: |
A detailed examination was made of the lipid content of sheep ovarian follicles maintained in organ culture, and of the effect on this of human chorionic gonadotrophin. Lipid classes were isolated by thin-layer chromatography and the fatty acid composition determined by gas chromatographic analysis. The mean total lipid content of isolated follicles, 4-6 mm in diameter, after 18 h in organ culture was 43'1 nmolfmg wet weight tissue. Five major lipid fractions were detected, namely phospholipids (21' 0 nmol/mg), cholesterol (14' 5 nmolfmg), triglycerides (3'1 nmol/mg), free fatty acids (1'1 nmol/mg), and cholesteryl esters (3' 4 nmolfmg). The phospholipid fraction was distinctive in that it contained a large percentage of sphingomyelin (28 '0%). Other phospholipids present were phosphatidyl choline (42'0%), phosphatidyl inositol (6'9%), phosphatidyl serine (6,1 %), and phosphatidyl ethanolamine and phosphatidic acid (16'9%). Each lipid fraction had a characteristic fatty acid profile which was rich in the content of unsaturated fatty acids. Inclusion of human chorionic gonadotrophin (20 i.u. ml") in the culture medium caused no discernable effects on the follicular content of lipids nor on the fatty acid composition of individual lipid fractions |
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Palavras-Chave: |
Folículos ovarianos; Gonadatrofina; HCG. |
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Thesagro: |
Endocrinologia; Lipídio; Ovário; Ovelha; Ovino. |
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Thesaurus Nal: |
Ewes; Gonadotropins; Lipid content; Ovarian follicles; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02000naa a2200289 a 4500
001 2053505
005 2016-10-26
008 1978 bl uuuu u00u1 u #d
100 1 $aHAMILTON, R. P.
245 $aLipid content of sheep ovarian follicles in culture.
260 $c1978
520 $aA detailed examination was made of the lipid content of sheep ovarian follicles maintained in organ culture, and of the effect on this of human chorionic gonadotrophin. Lipid classes were isolated by thin-layer chromatography and the fatty acid composition determined by gas chromatographic analysis. The mean total lipid content of isolated follicles, 4-6 mm in diameter, after 18 h in organ culture was 43'1 nmolfmg wet weight tissue. Five major lipid fractions were detected, namely phospholipids (21' 0 nmol/mg), cholesterol (14' 5 nmolfmg), triglycerides (3'1 nmol/mg), free fatty acids (1'1 nmol/mg), and cholesteryl esters (3' 4 nmolfmg). The phospholipid fraction was distinctive in that it contained a large percentage of sphingomyelin (28 '0%). Other phospholipids present were phosphatidyl choline (42'0%), phosphatidyl inositol (6'9%), phosphatidyl serine (6,1 %), and phosphatidyl ethanolamine and phosphatidic acid (16'9%). Each lipid fraction had a characteristic fatty acid profile which was rich in the content of unsaturated fatty acids. Inclusion of human chorionic gonadotrophin (20 i.u. ml") in the culture medium caused no discernable effects on the follicular content of lipids nor on the fatty acid composition of individual lipid fractions
650 $aEwes
650 $aGonadotropins
650 $aLipid content
650 $aOvarian follicles
650 $aSheep
650 $aEndocrinologia
650 $aLipídio
650 $aOvário
650 $aOvelha
650 $aOvino
653 $aFolículos ovarianos
653 $aGonadatrofina
653 $aHCG
700 1 $aSEAMARK, R. F.
773 $tAustralian Journal of Biological Sciences$gv. 31, n. 6, p. 621-628, Dec. 1978.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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