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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Soja. |
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Data corrente: |
03/11/1992 |
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Data da última atualização: |
09/02/2012 |
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Autoria: |
UPADHYAYA, N. M.; SCOTT, K. F.; TUCKER, W. T.; WATSON, J. M.; DART, P. J. |
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Afiliação: |
CSIRO Divison of Plant Industry, GPO Box 1600 Canberra, ACT 2601, Australia. |
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Título: |
Isolation and characterization of Rhizobium (IC3342) genes that determine leaf curl induction in pigeon pea. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Molecular Plant-Microbe Interactions, v.5, n.2, p.129-143, 1992. |
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Idioma: |
Inglês |
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Conteúdo: |
Nodulation by the Rhizobium strain IC3342 causes a leaf curl syndrome in certain tropical legumes such a pigeon pea (Cajanus cajan) (N.M.Upadhyaya, et al, Physiological and Molecular Plant Pathology 39:357-373, 1991). Transposon (Tn5) mutagenesis of this leaf curl-inducing (Curl+) Rhizobium strain yielded two Curl-Fix- and three Curl-Fix+ mutants. Plasmid visualization and subsequent Southern blot hybridization analyses with Tn5, nif and nod gene probes showed that the Tn5 element had inserted into the symbiotic (Sym) palmid in three of the mutants. Restriction endonuclease analyses indicated that none of the Tn5 insertions were closely linked. Tn5-containing EcoRI fragments were cloned from each mutant and used a probes to isolate the corresponding wild-typeDNA fragments from a cosmid (pLAFR3) genomic library. Fix+ and/or Curl+ phenotypes were restored in each mutant by the introduction of cosmids containing the corresponding wild-type DNA. A closely related byt Curl- Rhizobium strain ANU 240 was shown, by Southern hybridization, to contain conserved DNA sequences of all but one of the identified genetic regions of the Curl+ Thizobium strain IC3342. Cosmids containing the genetic regio unique to the strain IC3342, designated lcr1, conferred a Curl+ phenotype on the strain ANU240. DNA sequence analysis of the cloned lcr1 region revealed five open reading frames (ORFs). The ORF2 showed homology with the Escherichia coli regularoty gene ompR, and ORF4 showed homology with E. coli and Rhizobium meliloti regulatory MenosNodulation by the Rhizobium strain IC3342 causes a leaf curl syndrome in certain tropical legumes such a pigeon pea (Cajanus cajan) (N.M.Upadhyaya, et al, Physiological and Molecular Plant Pathology 39:357-373, 1991). Transposon (Tn5) mutagenesis of this leaf curl-inducing (Curl+) Rhizobium strain yielded two Curl-Fix- and three Curl-Fix+ mutants. Plasmid visualization and subsequent Southern blot hybridization analyses with Tn5, nif and nod gene probes showed that the Tn5 element had inserted into the symbiotic (Sym) palmid in three of the mutants. Restriction endonuclease analyses indicated that none of the Tn5 insertions were closely linked. Tn5-containing EcoRI fragments were cloned from each mutant and used a probes to isolate the corresponding wild-typeDNA fragments from a cosmid (pLAFR3) genomic library. Fix+ and/or Curl+ phenotypes were restored in each mutant by the introduction of cosmids containing the corresponding wild-type DNA. A closely related byt Curl- Rhizobium strain ANU 240 was shown, by Southern hybridization, to contain conserved DNA sequences of all but one of the identified genetic regions of the Curl+ Thizobium strain IC3342. Cosmids containing the genetic regio unique to the strain IC3342, designated lcr1, conferred a Curl+ phenotype on the strain ANU240. DNA sequence analysis of the cloned lcr1 region revealed five open reading frames (ORFs). The ORF2 showed homology with the Escherichia coli regularoty gene ompR, and ORF4 showed homology with E. c... Mostrar Tudo |
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Palavras-Chave: |
Cytokinin; Radioimmunoassay; RIZOBIO; Tn5 mutagenesis. |
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Thesagro: |
Siratro. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02191naa a2200229 a 4500
001 1458301
005 2012-02-09
008 1992 bl --- 0-- u #d
100 1 $aUPADHYAYA, N. M.
245 $aIsolation and characterization of Rhizobium (IC3342) genes that determine leaf curl induction in pigeon pea.
260 $c1992
520 $aNodulation by the Rhizobium strain IC3342 causes a leaf curl syndrome in certain tropical legumes such a pigeon pea (Cajanus cajan) (N.M.Upadhyaya, et al, Physiological and Molecular Plant Pathology 39:357-373, 1991). Transposon (Tn5) mutagenesis of this leaf curl-inducing (Curl+) Rhizobium strain yielded two Curl-Fix- and three Curl-Fix+ mutants. Plasmid visualization and subsequent Southern blot hybridization analyses with Tn5, nif and nod gene probes showed that the Tn5 element had inserted into the symbiotic (Sym) palmid in three of the mutants. Restriction endonuclease analyses indicated that none of the Tn5 insertions were closely linked. Tn5-containing EcoRI fragments were cloned from each mutant and used a probes to isolate the corresponding wild-typeDNA fragments from a cosmid (pLAFR3) genomic library. Fix+ and/or Curl+ phenotypes were restored in each mutant by the introduction of cosmids containing the corresponding wild-type DNA. A closely related byt Curl- Rhizobium strain ANU 240 was shown, by Southern hybridization, to contain conserved DNA sequences of all but one of the identified genetic regions of the Curl+ Thizobium strain IC3342. Cosmids containing the genetic regio unique to the strain IC3342, designated lcr1, conferred a Curl+ phenotype on the strain ANU240. DNA sequence analysis of the cloned lcr1 region revealed five open reading frames (ORFs). The ORF2 showed homology with the Escherichia coli regularoty gene ompR, and ORF4 showed homology with E. coli and Rhizobium meliloti regulatory
650 $aSiratro
653 $aCytokinin
653 $aRadioimmunoassay
653 $aRIZOBIO
653 $aTn5 mutagenesis
700 1 $aSCOTT, K. F.
700 1 $aTUCKER, W. T.
700 1 $aWATSON, J. M.
700 1 $aDART, P. J.
773 $tMolecular Plant-Microbe Interactions$gv.5, n.2, p.129-143, 1992.
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Registro original: |
Embrapa Soja (CNPSO) |
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