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Registro Completo |
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
16/12/2014 |
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Data da última atualização: |
16/12/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FARIA, A. C. S.; SCHWARZ, D. G. G.; CARVALHO, I. A.; ROCHA, B. B.; CASTRO, K. N. de C.; SILVA, M. R.; MOREIRA, M. A. S. |
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Afiliação: |
KARINA NEOOB DE CARVALHO CASTRO, CPAMN; MYRO RODRIGUES SILVA, CNPGL. |
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Título: |
Short communication: Viable Mycobacterium avium subspecies paratuberculosis in retail artisanal Coalho cheese from Northeastern Brazil. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 97, p. 4111-4114, 2014. |
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DOI: |
http://dx.doi.org/ 10.3168/jds.2013-7835 |
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Idioma: |
Inglês |
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Conteúdo: |
Mycobacterium avium ssp. paratuberculosis (MAP) is the etiologic agent of paratuberculosis and it potentially plays a role in Crohn?s disease. In humans, the main route of transmission of MAP might be the intake of contaminated milk and dairy products. Considering that MAP has already been detected in many types of cheese in different counties, and that Coalho cheese is an important dairy product in northeastern Brazil, the aim of this study was to report the first detection of MAP in retail Coalho cheese in Brazil by PCR and culture. Of 30 retail Coalho cheese samples, 3 (10%) amplified fragments of a similar size to that expected (626 bp) were obtained and viable MAP was recovered by culture from 1 (3.3%) sample. The DNA from the positive culture sample was sequenced and showed 99% identity with the insertion sequence IS900 deposited in GenBank. It was possible to identify the presence of MAP-specific DNA in the analyzed samples for the first time in Brazil, and to recover viable cells from retail Coalho cheese. |
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Thesagro: |
Queijo. |
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Thesaurus Nal: |
paratuberculosis. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/113903/1/JDairyScience.pdf
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Marc: |
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001 2002793
005 2014-12-16
008 2014 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/ 10.3168/jds.2013-7835$2DOI
100 1 $aFARIA, A. C. S.
245 $aShort communication$bViable Mycobacterium avium subspecies paratuberculosis in retail artisanal Coalho cheese from Northeastern Brazil.$h[electronic resource]
260 $c2014
520 $aMycobacterium avium ssp. paratuberculosis (MAP) is the etiologic agent of paratuberculosis and it potentially plays a role in Crohn?s disease. In humans, the main route of transmission of MAP might be the intake of contaminated milk and dairy products. Considering that MAP has already been detected in many types of cheese in different counties, and that Coalho cheese is an important dairy product in northeastern Brazil, the aim of this study was to report the first detection of MAP in retail Coalho cheese in Brazil by PCR and culture. Of 30 retail Coalho cheese samples, 3 (10%) amplified fragments of a similar size to that expected (626 bp) were obtained and viable MAP was recovered by culture from 1 (3.3%) sample. The DNA from the positive culture sample was sequenced and showed 99% identity with the insertion sequence IS900 deposited in GenBank. It was possible to identify the presence of MAP-specific DNA in the analyzed samples for the first time in Brazil, and to recover viable cells from retail Coalho cheese.
650 $aparatuberculosis
650 $aQueijo
700 1 $aSCHWARZ, D. G. G.
700 1 $aCARVALHO, I. A.
700 1 $aROCHA, B. B.
700 1 $aCASTRO, K. N. de C.
700 1 $aSILVA, M. R.
700 1 $aMOREIRA, M. A. S.
773 $tJournal of Dairy Science$gv. 97, p. 4111-4114, 2014.
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Embrapa Meio-Norte (CPAMN) |
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| 2. |  | VENTURIN, M.; MIRANDA, C. R. de; MATTHIENSEN, A. Qualidade da água de nascentes superficiais de propriedades rurais do entorno do Parque Estadual Fritz Plaumann, Concórdia, SC. In: JORNADA DE INICIAÇÃO CIENTÍFICA (JINC), 7., 2013, Concórdia. Anais... Brasília, DF: Embrapa, 2013. p. 41-42.| Tipo: Artigo em Anais de Congresso |
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