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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
18/12/2023 |
Data da última atualização: |
18/12/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA, C. G. N. da; MONTEIRO, E. de C.; DINIZ, P. P.; TERRA, L. A.; SCHWAB, S.; REIS, V. M.; ARAUJO, J. L. S. de; URQUIAGA, S. |
Afiliação: |
CLEUDISON GABRIEL NASCIMENTO DA SILVA, UFRRJ; EDEVALDO DE CASTRO MONTEIRO, UFRRJ; PRISCILA PEREIRA DINIZ, UFRRJ; LEONARDO ARAUJO TERRA, UFRRJ; STEFAN SCHWAB, CNPAB; VERONICA MASSENA REIS, CNPAB; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; SEGUNDO SACRAMENTO U CABALLERO, CNPAB. |
Título: |
Designing and validation of specifc primers for the quantitative detection of bacteria in sugarcane inoculant. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 54, p. 2627–2640, 2023. |
ISSN: |
1678-4405 |
Idioma: |
Inglês |
Conteúdo: |
Endophytic diazotrophic plant growth-promoting bacteria Herbaspirillum rubrisubalbicans (HCC103), Herbaspirillum seropedicae (HRC54), Paraburkholderia tropica (Ppe8T), Gluconacetobacter diazotrophicus (Pal5T), and Nitrospirillum amazonense (CBAmC) have been used as inoculants for sugarcane. The genome sequences of these strains were used to design a set of specifc primers for the real-time PCR (qPCR) assay. Primer specifcity was confrmed by conventional PCR using the genomic DNAs of 25 related bacterial species and the fve target strains. The qPCR assays were conducted using root and shoot samples from two sugarcane varieties (RB867515 and RB92579). These samples were collected both with and without inoculation, using the target strains specifed in this study. The sugarcane plants were grown in a greenhouse, utilizing a substrate composed of sterile sand and vermiculite in a 2:1 ratio, for a duration of 55 days. The primers designed for this study successfully amplifed target DNA fragments from each of the bacterial species, enabling their diferentiation at the species level. The total bacterial population present in the sugarcane quantifed using qPCR was on average 105.2 cells g−1 of fresh tissue. Across both evaluated varieties, it was observed that the population of inoculated bacteria tended to decrease over time and became more concentrated in the sugarcane roots compared to the aerial parts. The qPCR results suggest that both the host and the microbes infuence the endophytic population and the bacterial number decreases with plant age. MenosEndophytic diazotrophic plant growth-promoting bacteria Herbaspirillum rubrisubalbicans (HCC103), Herbaspirillum seropedicae (HRC54), Paraburkholderia tropica (Ppe8T), Gluconacetobacter diazotrophicus (Pal5T), and Nitrospirillum amazonense (CBAmC) have been used as inoculants for sugarcane. The genome sequences of these strains were used to design a set of specifc primers for the real-time PCR (qPCR) assay. Primer specifcity was confrmed by conventional PCR using the genomic DNAs of 25 related bacterial species and the fve target strains. The qPCR assays were conducted using root and shoot samples from two sugarcane varieties (RB867515 and RB92579). These samples were collected both with and without inoculation, using the target strains specifed in this study. The sugarcane plants were grown in a greenhouse, utilizing a substrate composed of sterile sand and vermiculite in a 2:1 ratio, for a duration of 55 days. The primers designed for this study successfully amplifed target DNA fragments from each of the bacterial species, enabling their diferentiation at the species level. The total bacterial population present in the sugarcane quantifed using qPCR was on average 105.2 cells g−1 of fresh tissue. Across both evaluated varieties, it was observed that the population of inoculated bacteria tended to decrease over time and became more concentrated in the sugarcane roots compared to the aerial parts. The qPCR results suggest that both the host and the microbes infuence the endo... Mostrar Tudo |
Palavras-Chave: |
Diazotrophic bacteria; DPGPB quantifcation; Primers; Real Time PCR. |
Thesagro: |
Saccharum Officinarum. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02399naa a2200277 a 4500 001 2159851 005 2023-12-18 008 2023 bl uuuu u00u1 u #d 022 $a1678-4405 100 1 $aSILVA, C. G. N. da 245 $aDesigning and validation of specifc primers for the quantitative detection of bacteria in sugarcane inoculant.$h[electronic resource] 260 $c2023 520 $aEndophytic diazotrophic plant growth-promoting bacteria Herbaspirillum rubrisubalbicans (HCC103), Herbaspirillum seropedicae (HRC54), Paraburkholderia tropica (Ppe8T), Gluconacetobacter diazotrophicus (Pal5T), and Nitrospirillum amazonense (CBAmC) have been used as inoculants for sugarcane. The genome sequences of these strains were used to design a set of specifc primers for the real-time PCR (qPCR) assay. Primer specifcity was confrmed by conventional PCR using the genomic DNAs of 25 related bacterial species and the fve target strains. The qPCR assays were conducted using root and shoot samples from two sugarcane varieties (RB867515 and RB92579). These samples were collected both with and without inoculation, using the target strains specifed in this study. The sugarcane plants were grown in a greenhouse, utilizing a substrate composed of sterile sand and vermiculite in a 2:1 ratio, for a duration of 55 days. The primers designed for this study successfully amplifed target DNA fragments from each of the bacterial species, enabling their diferentiation at the species level. The total bacterial population present in the sugarcane quantifed using qPCR was on average 105.2 cells g−1 of fresh tissue. Across both evaluated varieties, it was observed that the population of inoculated bacteria tended to decrease over time and became more concentrated in the sugarcane roots compared to the aerial parts. The qPCR results suggest that both the host and the microbes infuence the endophytic population and the bacterial number decreases with plant age. 650 $aSaccharum Officinarum 653 $aDiazotrophic bacteria 653 $aDPGPB quantifcation 653 $aPrimers 653 $aReal Time PCR 700 1 $aMONTEIRO, E. de C. 700 1 $aDINIZ, P. P. 700 1 $aTERRA, L. A. 700 1 $aSCHWAB, S. 700 1 $aREIS, V. M. 700 1 $aARAUJO, J. L. S. de 700 1 $aURQUIAGA, S. 773 $tBrazilian Journal of Microbiology$gv. 54, p. 2627–2640, 2023.
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Embrapa Agrobiologia (CNPAB) |
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1. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | STRECK, N. A.; UHLMANN, L. O.; GABRIEL, L. F. Long-term changes in rice development in Southern Brazil, during the last ten decades. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 47, n. 6, p. 727-737, jun. 2012. Título em português: Alterações de longo prazo nas fases de desenvolvimento de arroz no Sul do Brasil, nas últimas dez décadas.Biblioteca(s): Embrapa Unidades Centrais. |
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2. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | STRECK, N. A.; BURIOL, G. A.; HELDWEIN, A. B.; GABRIEL, L. F.; PAULA, G. M. de. Associação da variabilidade da precipitação pluvial em Santa Maria com a Oscilação Decadal do Pacífico. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 12, p. 1553-1561, dez. 2009 Título em inglês: Linking rainfall variability in Santa Maria with the Pacific Decadal Oscillation.Biblioteca(s): Embrapa Unidades Centrais. |
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4. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | STRECK, N. A.; LAGO, I.; GABRIEL, L. F.; SAMBORANHA, F. K. Simulating maize phenology as a function of air temperature with a linear and a nonlinear model. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 4, p. 449-455, abr. 2008 Título em português: Simulação da fenologia do milho em função da temperatura do ar por um modelo linear e um não linear.Biblioteca(s): Embrapa Unidades Centrais. |
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5. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | STRECK, N. A.; GABRIEL, L. F.; BURIOL, G. A.; HELDWEIN, A. B.; PAULA, G. M. de. Variabilidade interdecadal na série secular de temperatura do ar em Santa Maria, RS. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 46, n. 8, p. 781-790, ago. 2011. Título em inglês: Interdecadal variability in the secular series of air temperature in Santa Maria, RS, Brazil.Biblioteca(s): Embrapa Unidades Centrais. |
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