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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
20/10/2023 |
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Data da última atualização: |
20/10/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, C. G. N. da; MONTEIRO, E. de C.; DINIZ, P. P.; TERRA, L. A.; SCHWAB, S.; REIS, V. M.; ARAUJO, J. L. S. de; URQUIAGA, S. |
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Afiliação: |
CLEUDISON GABRIEL NASCIMENTO DA SILVA, UFRRJ; EDEVALDO DE CASTRO MONTEIRO, UFRRJ; PRISCILA PEREIRA DINIZ, UFRRJ; LEONARDO ARAUJO TERRA, UFRRJ; STEFAN SCHWAB, CNPAB; VERONICA MASSENA REIS, CNPAB; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; SEGUNDO SACRAMENTO U CABALLERO, CNPAB. |
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Título: |
Designing and validation of specific primers for the quantitative detection of bacteria in sugarcane inoculant. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology,, Published: 16 October 2023 |
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ISSN: |
1678-4405 |
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Idioma: |
Inglês |
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Conteúdo: |
Endophytic diazotrophic plant growth-promoting bacteria Herbaspirillum rubrisubalbicans (HCC103), Herbaspirillum seropedicae (HRC54), Paraburkholderia tropica (Ppe8 T ), Gluconacetobacter diazotrophicus (Pal5 T ), and Nitrospirillum amazonense (CBAmC) have been used as inoculants for sugarcane. The genome sequences of these strains were used to design a set of specific primers for the real-time PCR (qPCR) assay. Primer specificity was confirmed by conventional PCR using the genomic DNAs of 25 related bacterial species and the five target strains. The qPCR assays were conducted using root and shoot samples from two sugarcane varieties (RB867515 and RB92579). These samples were collected both with and without inoculation, using the target strains specified in this study. The sugarcane plants were grown in a greenhouse, utilizing a substrate composed of sterile sand and vermiculite in a 2:1 ratio, for a duration of 55 days. The primers designed for this study successfully amplified target DNA fragments from each of the bacterial species, enabling their differentiation at the species level. The total bacterial population present in the sugarcane quantified using qPCR was on average 105.2 cells g−1 of fresh tissue. Across both evaluated varieties, it was observed that the population of inoculated bacteria tended to decrease over time and became more concentrated in the sugarcane roots compared to the aerial parts. The qPCR results suggest that both the host and the microbes influence the endophytic population and the bacterial number decreases with plant age MenosEndophytic diazotrophic plant growth-promoting bacteria Herbaspirillum rubrisubalbicans (HCC103), Herbaspirillum seropedicae (HRC54), Paraburkholderia tropica (Ppe8 T ), Gluconacetobacter diazotrophicus (Pal5 T ), and Nitrospirillum amazonense (CBAmC) have been used as inoculants for sugarcane. The genome sequences of these strains were used to design a set of specific primers for the real-time PCR (qPCR) assay. Primer specificity was confirmed by conventional PCR using the genomic DNAs of 25 related bacterial species and the five target strains. The qPCR assays were conducted using root and shoot samples from two sugarcane varieties (RB867515 and RB92579). These samples were collected both with and without inoculation, using the target strains specified in this study. The sugarcane plants were grown in a greenhouse, utilizing a substrate composed of sterile sand and vermiculite in a 2:1 ratio, for a duration of 55 days. The primers designed for this study successfully amplified target DNA fragments from each of the bacterial species, enabling their differentiation at the species level. The total bacterial population present in the sugarcane quantified using qPCR was on average 105.2 cells g−1 of fresh tissue. Across both evaluated varieties, it was observed that the population of inoculated bacteria tended to decrease over time and became more concentrated in the sugarcane roots compared to the aerial parts. The qPCR results suggest that both the host and the microbes... Mostrar Tudo |
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Palavras-Chave: |
Diazotrophic bacteria; DPGPB quantification; Primers; Real Time PCR. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02378naa a2200265 a 4500
001 2157398
005 2023-10-20
008 2023 bl uuuu u00u1 u #d
022 $a1678-4405
100 1 $aSILVA, C. G. N. da
245 $aDesigning and validation of specific primers for the quantitative detection of bacteria in sugarcane inoculant.$h[electronic resource]
260 $c2023
520 $aEndophytic diazotrophic plant growth-promoting bacteria Herbaspirillum rubrisubalbicans (HCC103), Herbaspirillum seropedicae (HRC54), Paraburkholderia tropica (Ppe8 T ), Gluconacetobacter diazotrophicus (Pal5 T ), and Nitrospirillum amazonense (CBAmC) have been used as inoculants for sugarcane. The genome sequences of these strains were used to design a set of specific primers for the real-time PCR (qPCR) assay. Primer specificity was confirmed by conventional PCR using the genomic DNAs of 25 related bacterial species and the five target strains. The qPCR assays were conducted using root and shoot samples from two sugarcane varieties (RB867515 and RB92579). These samples were collected both with and without inoculation, using the target strains specified in this study. The sugarcane plants were grown in a greenhouse, utilizing a substrate composed of sterile sand and vermiculite in a 2:1 ratio, for a duration of 55 days. The primers designed for this study successfully amplified target DNA fragments from each of the bacterial species, enabling their differentiation at the species level. The total bacterial population present in the sugarcane quantified using qPCR was on average 105.2 cells g−1 of fresh tissue. Across both evaluated varieties, it was observed that the population of inoculated bacteria tended to decrease over time and became more concentrated in the sugarcane roots compared to the aerial parts. The qPCR results suggest that both the host and the microbes influence the endophytic population and the bacterial number decreases with plant age
653 $aDiazotrophic bacteria
653 $aDPGPB quantification
653 $aPrimers
653 $aReal Time PCR
700 1 $aMONTEIRO, E. de C.
700 1 $aDINIZ, P. P.
700 1 $aTERRA, L. A.
700 1 $aSCHWAB, S.
700 1 $aREIS, V. M.
700 1 $aARAUJO, J. L. S. de
700 1 $aURQUIAGA, S.
773 $tBrazilian Journal of Microbiology,, Published: 16 October 2023
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Biblioteca |
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| Registros recuperados : 112 | |
| 81. |  | SCHWAB, S.; PESSOA, C. A.; BERGAMI, A. A. de L.; FIGUEIREDO, N. L. A.; TEIXEIRA, K. R. dos S.; BALDANI, J. I. Isolation and characterization of active promoters from Gluconacetobacter diazotrophicus strain PAL5 using a promoter-trapping plasmid. Archives of Microbiology, v. 198, n. 5, p. 445?458, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 82. | | FERREIRA, N. S.; MATOS, G. F.; MENESES, C. H. G. de; REIS, V. M.; ROUWS, J. R. C.; SCHWAB, S.; BALDANI, J. I.; ROUWS, L. F. M. Interaction of phytohormone-producing rhizobia with sugarcane mini-setts and their effect on plant development. Plant and Soil, Published online 3 January, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 83. | | TERRA, L. A.; SOARES, C. de P.; TADRA-SFEIR, M. Z.; SOUZA, E. M.; SILVEIRA, V.; BALDANI, J. I.; VIDAL, M. S.; SCHWAB, S. Transcriptome and proteome approaches for the diazotroph Nitrospirillum amazonense grown in sugarcane apoplast fluid. In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 16., LATINAMERICAN WORKSHOP OF PGPR, 4., RELARE, 19., 2018, Foz do Iguaçu. Book of abstract....Foz do Iguaçu: Emprapa, 2018. p. 52 Session 4, Poster IV.5| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 84. | | TERRA, L. A.; SOARES, C. de P.; MENESES, C. H. G. de; SFEIR, T.; SOUZA, E. M. de; SILVEIRA, V.; VIDAL, M. S.; BALDANI, J. I.; SCHWAB, S. Transcriptome and proteome profiles of the diazotroph Nitrospirillum amazonense strain CBAmC in response to the sugarcane apoplast fluid. Plant and Soil, v. 451, p. 145-168, 2020.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 85. | | SANTOS, C. M. dos; BALSANELLI, E.; SOUZA, E. M.; RIBERIRO, N. V. S.; SCHWAB, S.; VIDAL, M. S.; BALDANI, J. I. Understanding the apoplast bacterial community from sugarcane inoculated with a consortium of fi ve diazotrophic bacteria. In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 16.; LATINAMERICAN WORKSHOP OF PGPR, 4.; RELARE, 19., 2018, Foz do Iguaçu. Book of abstract....Foz do Iguaçu: Embrapa, 2018. p. 79 Session 6, Poster VI.33| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 86. | | BALDANI, J. I.; GUEDES, H. V.; ROUWS, L. F. M.; VIDAL, M. S.; ARAUJO, J. L. S. de; SCHWAB, S.; TEIXEIRA, K. R. dos S. Gluconacetobacter dizotrophicus genome and its potential for biotechnological apllication In: REUNIÓN CIENTIFICO TÉCNICA DE BIOLOGIA DEL SUELO Y FIJACIÓN BIOLÓGICA DE NITRÓGENIO- REBIOS, 7., 2009, San Miguel de Tucaumán, AR. Libro de resúmenes...San Miguel de Tucaumán: Universidad Nacional de Tucumán, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 87. | | SILVA, P. R. A. da; SCHWAB, S.; ARAUJO, J. L. S. de; TEIXEIRA, K. R. dos S.; VIDAL, M. S.; BALDANI, J. I. Genômica funcional da bactéria diazotrófica Burkholderia tropica cultivada em líquido de apoplasto de cana-de-açúcar In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 12., 2012. Economia verde, sustentabilidade e erradicação da pobreza: caderno de resumos. Seropédica: Embrapa Agrobiologia, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 88. | | CAMELO, A.; BARRETO, C. P.; VIDAL, M. S.; ROUWS, J. R. C.; LEDO, F. J. da S.; SCHWAB, S.; BALDANI, J. I. Field response of two seed propagated elephant grass genotypes to diazotrophic bacterial inoculation and in situ confocal microscopy colonization analyses. Symbiosis, v. 83, n. 1, p. 41-53, 2021| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia; Embrapa Gado de Leite. |
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| 89. | | GUEDES, H. V.; ROUWS, L. F. M.; ALQUERES, S. M. C.; MENESES, C. H. S. G.; VIDAL, M. S.; MARTINS, O. B.; SCHWAB, S.; BALDANI, J. I. Histidine kinase gene might be involved in biological nirogen fixation and rice root colonization by gluconacetobacter diazotophicus PAL5 In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. Sl. 14| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 90. | | SILVA, C. G. N. da; MONTEIRO, E. de C.; DINIZ, P. P.; TERRA, L. A.; SCHWAB, S.; REIS, V. M.; ARAUJO, J. L. S. de; URQUIAGA, S. Designing and validation of specifc primers for the quantitative detection of bacteria in sugarcane inoculant. Brazilian Journal of Microbiology, v. 54, p. 2627–2640, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 91. | | SILVA, C. G. N. da; MONTEIRO, E. de C.; DINIZ, P. P.; TERRA, L. A.; SCHWAB, S.; REIS, V. M.; ARAUJO, J. L. S. de; URQUIAGA, S. Designing and validation of specific primers for the quantitative detection of bacteria in sugarcane inoculant. Brazilian Journal of Microbiology,, Published: 16 October 2023| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 92. | | SANTOS, C. M dos; SOUZA, D. G. de; BALSANELLI, E; CRUZ, L. M.; SOUZA, E. M. de; BALDANI, J. I.; SCHWAB, S. A culture independent approach to enrich endophytic bacterial cells from sugarcane stems for community characterization. Microbiol Ecology, v. 74, n. 2, p. 453-465, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 93. | | BALDANI, J. I.; CRUZ, L. M.; VIDAL, M. S.; ARAUJO, J. L. S. de; SCHWAB, S.; TEIXEIRA, K. R. dos S.; SOUZA, E. M.; PEDROSA, F. O.; FARINELLI. Draft genome sequente of six endophytic nittrogen-fixing bacteria with potential biofertilizer application in non-leguminous plants. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. Slll. 05| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 94. | | REIS, V. M.; PITARD, R. M.; DOURADO, F. dos S.; CAMACHO, N. N.; VIDEIRA, S. S.; CAMARGO, A. B.; BOA SORTE, P. F.; VIDAL, M. S.; SCHWAB, S.; BALDANI, J. I. Caracterização morfo-fisiológica e molecular das estirpres diazotróficas do inoculante de cana-de-açúcar. Seropédica: Embrapa Agrobiologia, 2011. (Embrapa Agrobiologia. Documentos, 280).| Biblioteca(s): Embrapa Agrobiologia. |
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| 95. | | SCHWAB, S.; CRIVELARO, A. L. R.; WEISS, V. A.; SFEIR, M. Z. T.; FAORO, H.; CRUZ, L. M.; PEDROZA, F. de O.; SOUZA, E. M. de; BALDANI, J. I. Global transcriptional shift analysis of nitrogenase switch-off by glutamate in azospirillum amazonense strain CBAMC, isolated from sugarcane. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION WITH NON-LEGUMES, 13., 2012, Neuherberg, GE. Abstracts... Neuherberg: German Research Center for Environmental Health, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 96. | | ROUWS, L. F. M.; MENESES, C. H. G.; GALVÃO, P. G.; VENTURA, H, G.; DRECHSEL, M. M.; SILVA, R. J.; VIDAL, M. S.; SIMÕES-ARAUJO, J. L.; TEIXEIRA, K. R. S.; SCHWAB, S.; BALDANI, J. I. Functional analysis of Gluconacetobacter diazotrophicus PAL5 genes involved in the nitogen fixation process and interaction with the plant. INTERNATIONAL INCT SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 1., 2009, Curitiba. Program and index... Brasilia: INCT, 2009. 01p| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 97. | | ROUWS, L. F. M.; MENESSES, C. H. G.; GALVÃO, P. G.; VENTURA, H. G.; DRECHSEL, M. M.; SILVA, R. J.; VIDAL, M. S.; SIMÕES-ARAUJO, J. L.; TEIXEIRA, K. R. S.; SCHWAB, S.; BALDANI, J. I. Functional analysis of Gluconacetobacter diazotrophicus PAL5 genes involved in the nitrogen fixation processs and interaction with the plant. INTERNATIONAL INCT SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 1., 2009, Curitiba. Program and index... Brasilia: INCT, 2009. 01p| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 98. | | BALDANI, J. I.; VIDEIRA, S. S.; TEIXEIRA, K. R. dos S.; REIS, V. M.; OLIVERIA, A. L. M. de; SCHWAB, S.; SOUZA, E. M.; PEDRAZA, R. O.; BALDANI, V. L. D.; HARTMANN, A. The family Rhodospirillaceae. In: ROSENBERG, E. (Ed.). The prokaryotes: Alphaproteobacteria and Betaproteobacteria. 4. ed. Heidelberg: Springer, 2014. p. 533-618| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 100. | | SCHWAB, S.; HIRATA, E. S.; AMARAL, J. C. A.; SILVA, C. G. N.; FERREIRA, J. P.; SILVA, L. V. da; ROUWS, J. R. C.; ROUWS, L. F. M.; BALDANI, J. I.; REIS, V. M. Quantifying and visualizing Nitrospirillum amazonense strain CBAmC in sugarcane after using different inoculation methods. Plant and Soil , Published: 22 February 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 112 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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