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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
13/08/2019 |
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Data da última atualização: |
26/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FRANCISCO, R.; BRANCO, R.; SCHWAB, S.; BALDANI, J. I.; MORAIS, P. V. |
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Afiliação: |
Romeu Francisco, University of Coimbra; Rita Branco, University of Coimbra; STEFAN SCHWAB, CNPAB; JOSE IVO BALDANI, CNPAB; Paula V. Morais1,2, BOLSISTA EMBRAPA AGROBIOLOGIA. |
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Título: |
Two plant hosted whole cell bacterial biosensors for detection of bioavailable Cr(VI). |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
World Journal of Microbiology and Biotechnology, v. 35, n. p. 129-143, 2019. |
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DOI: |
https://doi.org/10.1007/s11274-019-2703-0 |
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Idioma: |
Inglês |
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Conteúdo: |
Metal whole cell biosensors (WCBs) have been reported as very useful tools to detect and quantify the presence of bioavailable fractions of certain metals in water and soil samples. In the current work, two bacterial WCBs able to report Cr(VI) presence and plants growing on Cr(VI)-enriched soil/medium were used to assess the potential transfer of this metal to organisms of higher trophic levels, and the risk of transfer to the food chain. To do it, the functionality of the WCBs within tissues of inoculated plants in contact with Cr(VI)-contaminated soil and water was studied in vitro and in a controlled greenhouse environment. One WCB was the previously described Ochrobactrum tritici pCHRGFP2 and the second, Nitrospirillum amazonense pCHRGFP2, is a newly engineered naturally-occurring endophytic microorganism. Three rice varieties (IAC 4440, BRS 6 CHUÍ, IRGA 425) and one maize variety (1060) were tested as hosts and subjected to Cr(VI) treatments (25 ?M), with different results obtained. Inoculation of each WCB into plants exposed to Cr(VI) showed GFP expression within plant tissues. WCBs penetrated the root tissues and later colonized the shoots and leaves. In general, a higher fluorescence signal was detected in roots, together with a higher Cr content and denser WCB colonization. Best fluorescence intensities per plant biomass of shoots were obtained for plant host IRGA 425. Therefore, by analyzing colonized tissues, both WCBs allowed the detection of Cr(VI) contamination in soils and its transfer to plants commonly used in crops for human diet. MenosMetal whole cell biosensors (WCBs) have been reported as very useful tools to detect and quantify the presence of bioavailable fractions of certain metals in water and soil samples. In the current work, two bacterial WCBs able to report Cr(VI) presence and plants growing on Cr(VI)-enriched soil/medium were used to assess the potential transfer of this metal to organisms of higher trophic levels, and the risk of transfer to the food chain. To do it, the functionality of the WCBs within tissues of inoculated plants in contact with Cr(VI)-contaminated soil and water was studied in vitro and in a controlled greenhouse environment. One WCB was the previously described Ochrobactrum tritici pCHRGFP2 and the second, Nitrospirillum amazonense pCHRGFP2, is a newly engineered naturally-occurring endophytic microorganism. Three rice varieties (IAC 4440, BRS 6 CHUÍ, IRGA 425) and one maize variety (1060) were tested as hosts and subjected to Cr(VI) treatments (25 ?M), with different results obtained. Inoculation of each WCB into plants exposed to Cr(VI) showed GFP expression within plant tissues. WCBs penetrated the root tissues and later colonized the shoots and leaves. In general, a higher fluorescence signal was detected in roots, together with a higher Cr content and denser WCB colonization. Best fluorescence intensities per plant biomass of shoots were obtained for plant host IRGA 425. Therefore, by analyzing colonized tissues, both WCBs allowed the detection of Cr(VI) contamination... Mostrar Tudo |
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Palavras-Chave: |
Nitrospirillum amazonense; Wholecell biosensors. |
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Thesaurus Nal: |
Chromium; Rice soils. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02293naa a2200229 a 4500
001 2111307
005 2019-11-26
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11274-019-2703-0$2DOI
100 1 $aFRANCISCO, R.
245 $aTwo plant hosted whole cell bacterial biosensors for detection of bioavailable Cr(VI).$h[electronic resource]
260 $c2019
520 $aMetal whole cell biosensors (WCBs) have been reported as very useful tools to detect and quantify the presence of bioavailable fractions of certain metals in water and soil samples. In the current work, two bacterial WCBs able to report Cr(VI) presence and plants growing on Cr(VI)-enriched soil/medium were used to assess the potential transfer of this metal to organisms of higher trophic levels, and the risk of transfer to the food chain. To do it, the functionality of the WCBs within tissues of inoculated plants in contact with Cr(VI)-contaminated soil and water was studied in vitro and in a controlled greenhouse environment. One WCB was the previously described Ochrobactrum tritici pCHRGFP2 and the second, Nitrospirillum amazonense pCHRGFP2, is a newly engineered naturally-occurring endophytic microorganism. Three rice varieties (IAC 4440, BRS 6 CHUÍ, IRGA 425) and one maize variety (1060) were tested as hosts and subjected to Cr(VI) treatments (25 ?M), with different results obtained. Inoculation of each WCB into plants exposed to Cr(VI) showed GFP expression within plant tissues. WCBs penetrated the root tissues and later colonized the shoots and leaves. In general, a higher fluorescence signal was detected in roots, together with a higher Cr content and denser WCB colonization. Best fluorescence intensities per plant biomass of shoots were obtained for plant host IRGA 425. Therefore, by analyzing colonized tissues, both WCBs allowed the detection of Cr(VI) contamination in soils and its transfer to plants commonly used in crops for human diet.
650 $aChromium
650 $aRice soils
653 $aNitrospirillum amazonense
653 $aWholecell biosensors
700 1 $aBRANCO, R.
700 1 $aSCHWAB, S.
700 1 $aBALDANI, J. I.
700 1 $aMORAIS, P. V.
773 $tWorld Journal of Microbiology and Biotechnology$gv. 35, n. p. 129-143, 2019.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
18/12/2019 |
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Data da última atualização: |
18/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ARAUJO, K. E. C.; TORRES JUNIOR, C. V.; GUIMARÃES, A. P.; SILVA, M. A. da; ALVES, B. J. R.; URQUIAGA, S.; BODDEY, R. M. |
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Afiliação: |
Karla Emanuelle Campos Araujo; Carlos Vergara Torres Júnior, UFRRJ; Ana Paula Guimarães, UFRRJ; Mara Alexandre da Silva, UFRRJ; BRUNO JOSE RODRIGUES ALVES, CNPAB; SEGUNDO SACRAMENTO U CABALLERO, CNPAB; ROBERT MICHAEL BODDEY, CNPAB. |
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Título: |
Natural abundance of 15N of N derived from the atmosphere by different strains of Bradyrhizobium in symbiosis with soybean plants. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Ciência Rural, v. 49, n.12, e20180265, 2019. |
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Idioma: |
Inglês |
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Notas: |
Título em Português: Abundância natural de 15N do N derivado da atmosfera por diferentes estirpes de Bradyrhizobium em simbiose com plantas de soja. |
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Conteúdo: |
To quantify the BNF contribution to legumes using the 15N natural abundance technique, it is important to know the abundance of 15N of the plants grown entirely dependent on BNF (value ?B?). The aim of the study was to determine the 15N natural abundance of N2 fixed by different Bradyrhizobium strains in symbiosis with one soybean cultivar. Treatments consisted of soybean plants cultivated with and without inoculation with ten Bradyrhizobium strains, in five replicates planted in Leonard jars in a sand/vermiculite mixture. |
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Palavras-Chave: |
15N Isotopic fractionation; B Value; Biological nitrogen fixation. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 01427naa a2200241 a 4500
001 2117211
005 2019-12-18
008 2019 bl uuuu u00u1 u #d
100 1 $aARAUJO, K. E. C.
245 $aNatural abundance of 15N of N derived from the atmosphere by different strains of Bradyrhizobium in symbiosis with soybean plants.$h[electronic resource]
260 $c2019
500 $aTítulo em Português: Abundância natural de 15N do N derivado da atmosfera por diferentes estirpes de Bradyrhizobium em simbiose com plantas de soja.
520 $aTo quantify the BNF contribution to legumes using the 15N natural abundance technique, it is important to know the abundance of 15N of the plants grown entirely dependent on BNF (value ?B?). The aim of the study was to determine the 15N natural abundance of N2 fixed by different Bradyrhizobium strains in symbiosis with one soybean cultivar. Treatments consisted of soybean plants cultivated with and without inoculation with ten Bradyrhizobium strains, in five replicates planted in Leonard jars in a sand/vermiculite mixture.
653 $a15N Isotopic fractionation
653 $aB Value
653 $aBiological nitrogen fixation
700 1 $aTORRES JUNIOR, C. V.
700 1 $aGUIMARÃES, A. P.
700 1 $aSILVA, M. A. da
700 1 $aALVES, B. J. R.
700 1 $aURQUIAGA, S.
700 1 $aBODDEY, R. M.
773 $tCiência Rural$gv. 49, n.12, e20180265, 2019.
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