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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
15/04/2004 |
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Data da última atualização: |
15/04/2004 |
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Autoria: |
TAYLOR, N. J.; MASONA, M. V.; SCHÖPKE, C.; DABBHADATTA, Y.; THAO HO.; BEACHY, R. N.; FAUQUET, C. M. |
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Título: |
Induction of friable embryogenic tissues and plantlet regeneration in a range of cassava cultivars. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Revista Brasileira de Mandioca, Cruz das Almas, v. 17, p. 34, nov., 1998. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Friable embryogenic tissues and embryogenic suspension cultures are employed for the recovery of genetically transformed plants in many crop species and have been successfully used to regenerate transgenic cassava plants. The ability to produce and regenerate plants from these tissues is thus of central importance to their successful utilisation within cassava genetic transformation programmes and their potential exploitation as a system for mass clonal propagation. Research at ILTAB has generated friable embryogenic callus in 13 cassava cultivars, originating from Africa, South America and Asia. Long term embryogenic suspensions have been established and maintained in 10 of these , with studies in growth rate, size distribution and regeneration from the embryogenic units revealing differences across the cultivars. To date, mature embryos have been regenerated in 11 cultivars with subsequent germination and plantlet production from seven. Techniques for embryo maturation and germination have developed such that regeneration in the model cultivar TMS 60444 is now possible at more than 100 plantlets per gram of friable callus. Data will be presented concerning the progress made in the generation of friable embryogenic tissues and the methods employed for somatic embryo germination and subsequent plantlet establishment. Results from histological studies will be used to illustrate the developmental stages involved and the nature of cultivar variation at the cellular level. Implications for use of friable embryogenic tissues in genetic transformation programmes will also be presented. MenosAbstract: Friable embryogenic tissues and embryogenic suspension cultures are employed for the recovery of genetically transformed plants in many crop species and have been successfully used to regenerate transgenic cassava plants. The ability to produce and regenerate plants from these tissues is thus of central importance to their successful utilisation within cassava genetic transformation programmes and their potential exploitation as a system for mass clonal propagation. Research at ILTAB has generated friable embryogenic callus in 13 cassava cultivars, originating from Africa, South America and Asia. Long term embryogenic suspensions have been established and maintained in 10 of these , with studies in growth rate, size distribution and regeneration from the embryogenic units revealing differences across the cultivars. To date, mature embryos have been regenerated in 11 cultivars with subsequent germination and plantlet production from seven. Techniques for embryo maturation and germination have developed such that regeneration in the model cultivar TMS 60444 is now possible at more than 100 plantlets per gram of friable callus. Data will be presented concerning the progress made in the generation of friable embryogenic tissues and the methods employed for somatic embryo germination and subsequent plantlet establishment. Results from histological studies will be used to illustrate the developmental stages involved and the nature of cultivar variation at the cellular le... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02201naa a2200193 a 4500
001 1651912
005 2004-04-15
008 1998 bl uuuu u00u1 u #d
100 1 $aTAYLOR, N. J.
245 $aInduction of friable embryogenic tissues and plantlet regeneration in a range of cassava cultivars.
260 $c1998
520 $aAbstract: Friable embryogenic tissues and embryogenic suspension cultures are employed for the recovery of genetically transformed plants in many crop species and have been successfully used to regenerate transgenic cassava plants. The ability to produce and regenerate plants from these tissues is thus of central importance to their successful utilisation within cassava genetic transformation programmes and their potential exploitation as a system for mass clonal propagation. Research at ILTAB has generated friable embryogenic callus in 13 cassava cultivars, originating from Africa, South America and Asia. Long term embryogenic suspensions have been established and maintained in 10 of these , with studies in growth rate, size distribution and regeneration from the embryogenic units revealing differences across the cultivars. To date, mature embryos have been regenerated in 11 cultivars with subsequent germination and plantlet production from seven. Techniques for embryo maturation and germination have developed such that regeneration in the model cultivar TMS 60444 is now possible at more than 100 plantlets per gram of friable callus. Data will be presented concerning the progress made in the generation of friable embryogenic tissues and the methods employed for somatic embryo germination and subsequent plantlet establishment. Results from histological studies will be used to illustrate the developmental stages involved and the nature of cultivar variation at the cellular level. Implications for use of friable embryogenic tissues in genetic transformation programmes will also be presented.
700 1 $aMASONA, M. V.
700 1 $aSCHÖPKE, C.
700 1 $aDABBHADATTA, Y.
700 1 $aTHAO HO.
700 1 $aBEACHY, R. N.
700 1 $aFAUQUET, C. M.
773 $tRevista Brasileira de Mandioca, Cruz das Almas$gv. 17, p. 34, nov., 1998. Suplemento.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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