Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
12/08/1999 |
Data da última atualização: |
16/05/2023 |
Autoria: |
SCHOPKE, C.; TAYLOR, N. J.; CARCAMO, R.; BEACHY, R. N.; FAUQUET, C. |
Afiliação: |
International Laboratory for Tropical Agricultural Biotechnology. The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. |
Título: |
Optimization of parameters for particle bombardment of embryogenic suspension cultures of cassava (Manihot esculenta Crantz) using computer image analysis. |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
Plant Cell Reports, v.16, p.526-530, 1997. |
Idioma: |
Inglês |
Conteúdo: |
Tissue derived from embryogenic suspension cultures of cassava was bombarded with microparticles coated with a plasmid containing the uidA gene, which codes for glucuronidase (GUS). After 3 days, the effect of different bombardment parameters was evaluated by comparing the numbers of blue spots that resulted from histological GUS assays. Counting of blue spots was performed using a system comprised of a black and white video camera, a stereoscope and a personal computer. A reproducible counting method was established by optimizing GUS assay conditions, preparation of tissue samples and acquisition of video images in view of attaining the highest possible contrast between the blue spots and the surrounding tissue, The effects of bombardment pressure, microparticle size, number of bombardments, and osmotic pretreatment on GUS expression were investigated. |
Palavras-Chave: |
Embryogenic suspension cultures; Ferricyanide; Ferrocyanide; Glucuronidase; Microparticle bombardment. |
Thesaurus Nal: |
image analysis. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01632naa a2200241 a 4500 001 1648243 005 2023-05-16 008 1997 bl uuuu u00u1 u #d 100 1 $aSCHOPKE, C. 245 $aOptimization of parameters for particle bombardment of embryogenic suspension cultures of cassava (Manihot esculenta Crantz) using computer image analysis.$h[electronic resource] 260 $c1997 520 $aTissue derived from embryogenic suspension cultures of cassava was bombarded with microparticles coated with a plasmid containing the uidA gene, which codes for glucuronidase (GUS). After 3 days, the effect of different bombardment parameters was evaluated by comparing the numbers of blue spots that resulted from histological GUS assays. Counting of blue spots was performed using a system comprised of a black and white video camera, a stereoscope and a personal computer. A reproducible counting method was established by optimizing GUS assay conditions, preparation of tissue samples and acquisition of video images in view of attaining the highest possible contrast between the blue spots and the surrounding tissue, The effects of bombardment pressure, microparticle size, number of bombardments, and osmotic pretreatment on GUS expression were investigated. 650 $aimage analysis 653 $aEmbryogenic suspension cultures 653 $aFerricyanide 653 $aFerrocyanide 653 $aGlucuronidase 653 $aMicroparticle bombardment 700 1 $aTAYLOR, N. J. 700 1 $aCARCAMO, R. 700 1 $aBEACHY, R. N. 700 1 $aFAUQUET, C. 773 $tPlant Cell Reports$gv.16, p.526-530, 1997.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|