Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
22/04/1994 |
Data da última atualização: |
22/04/1994 |
Autoria: |
FAUQUET, C. M.; SCHOPKE, C.; CHAVARRIAGA, P.; SANGARE, A.; VEACHY, R. N. |
Afiliação: |
ILTAB/TSRI, La Jolla, CA, USA. |
Título: |
Genetic engineering technologies to control viruses and their application to cassava viruses. |
Ano de publicação: |
1993 |
Fonte/Imprenta: |
Cali, Colombia: CIAT, 1993 |
Páginas: |
190-207p |
Série: |
CIAT,Working Document, 123 |
Idioma: |
Inglês |
Notas: |
International scientific meeting Cassava Biotecnology Network, 1, 1992, Cartajena, Colombia. Proceedings... Cali, Colombia: CIAT, 1993. |
Conteúdo: |
Resistance to plant viruses through genetic engineering has been investigated with the application of four different approaches. A recent approach is the use of rigozymes, which involves using catalytic RNAs to cause the cleavage of viral RNA molecules. Results of in vitro experiments have documented cleavafe of viral RNAs but in vivo experiments has thus far not been reported. The second tecriques is to attempt to block the translation of viral RNAs by wxprossing genes that encode sequences complementary (i.e. antisense) to viral genes. Only a limited numger of cases have been reported, but little success has been documented. A third approach is to block virus replication, using competitor sequences, subgenomic sequences and satellite RNAs. The last techniq ue is the expression of viral genes which will interfere with virus replication and/or, diffusion in the plants; replicase gene, movement proteins and capsid proteins. The most promising techmique is that of the coat protein (CP), which consists of the integration of a gene encoding viral coat protein into the plant genome. An increasing number of examples using the coat protein mediated resistance (CP _ MR) strategy are avaliable, and resistance specificity and effecacy have been evaluated for viruses belonging to thirteen groups; furthermore, several successful field wxperiments have been conducted. |
Palavras-Chave: |
Casa de vegetacao; Congresso. |
Thesagro: |
Genoma; Vírus. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02166naa a2200253 a 4500 001 1644378 005 1994-04-22 008 1993 bl uuuu u00u1 u #d 100 1 $aFAUQUET, C. M. 245 $aGenetic engineering technologies to control viruses and their application to cassava viruses. 260 $c1993 300 $a190-207p 490 $aCIAT,Working Document, 123 500 $aInternational scientific meeting Cassava Biotecnology Network, 1, 1992, Cartajena, Colombia. Proceedings... Cali, Colombia: CIAT, 1993. 520 $aResistance to plant viruses through genetic engineering has been investigated with the application of four different approaches. A recent approach is the use of rigozymes, which involves using catalytic RNAs to cause the cleavage of viral RNA molecules. Results of in vitro experiments have documented cleavafe of viral RNAs but in vivo experiments has thus far not been reported. The second tecriques is to attempt to block the translation of viral RNAs by wxprossing genes that encode sequences complementary (i.e. antisense) to viral genes. Only a limited numger of cases have been reported, but little success has been documented. A third approach is to block virus replication, using competitor sequences, subgenomic sequences and satellite RNAs. The last techniq ue is the expression of viral genes which will interfere with virus replication and/or, diffusion in the plants; replicase gene, movement proteins and capsid proteins. The most promising techmique is that of the coat protein (CP), which consists of the integration of a gene encoding viral coat protein into the plant genome. An increasing number of examples using the coat protein mediated resistance (CP _ MR) strategy are avaliable, and resistance specificity and effecacy have been evaluated for viruses belonging to thirteen groups; furthermore, several successful field wxperiments have been conducted. 650 $aGenoma 650 $aVírus 653 $aCasa de vegetacao 653 $aCongresso 700 1 $aSCHOPKE, C. 700 1 $aCHAVARRIAGA, P. 700 1 $aSANGARE, A. 700 1 $aVEACHY, R. N. 773 $tCali, Colombia: CIAT, 1993
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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