Data/hora: 21/06/2024 03:27:31

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 12/08/1999
Data da última atualização: | 16/05/2023
Autoria: | SCHOPKE, C.; TAYLOR, N. J.; CARCAMO, R.; BEACHY, R. N.; FAUQUET, C.
Afiliação: | International Laboratory for Tropical Agricultural Biotechnology. The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Título: | Optimization of parameters for particle bombardment of embryogenic suspension cultures of cassava (Manihot esculenta Crantz) using computer image analysis.
Ano de publicação: | 1997
Fonte/Imprenta: | Plant Cell Reports, v.16, p.526-530, 1997.
Idioma: | Inglês
Conteúdo: | Tissue derived from embryogenic suspension cultures of cassava was bombarded with microparticles coated with a plasmid containing the uidA gene, which codes for glucuronidase (GUS). After 3 days, the effect of different bombardment parameters was evaluated by comparing the numbers of blue spots that resulted from histological GUS assays. Counting of blue spots was performed using a system comprised of a black and white video camera, a stereoscope and a personal computer. A reproducible counting method was established by optimizing GUS assay conditions, preparation of tissue samples and acquisition of video images in view of attaining the highest possible contrast between the blue spots and the surrounding tissue, The effects of bombardment pressure, microparticle size, number of bombardments, and osmotic pretreatment on GUS expression were investigated.
Palavras-Chave: | Embryogenic suspension cultures; Ferricyanide; Ferrocyanide; Glucuronidase; Microparticle bombardment.
Thesaurus Nal: | image analysis.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (17274-1) - ADD - AP - DD - Publicação digital -  -  - DIS