|
|
![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
25/09/2017 |
Data da última atualização: |
22/12/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MOREIRA, E. C. O.; PINHEIRO, D. G.; GORDO, S. M. C.; RODRIGUES, S. de M.; PESSOA, E.; SCHALLER, H.; LEMOS, O. F. de; SILVA, A.; SCHNEIDER, H.; SILVA JUNIOR, W. A.; SAMPAIO, I.; DARNET, S. |
Afiliação: |
Edith C. O. Moreira, UFPA; Daniel G. Pinheiro, USP/UNESP; Sheila M. C. Gordo, UFPA; SIMONE DE MIRANDA RODRIGUES, CPATU; Elaine Pessoa, UFPA / Laboratoire International Associe PALMHEAT CNRS/ UFPA, Recherche et Cooperation Internationale (DERCI); Hubert Schaller, Universite de Strasbourg / Laboratoire International Associe PALMHEAT CNRS/ UFPA, Recherche et Cooperation Internationale (DERCI); ORIEL FILGUEIRA DE LEMOS, CPATU; Artur Silva, UFPA; Horacio Schneider, UFPA; Wilson A. Silva Jr., USP; Iracilda Sampaio, UFPA; Sylvain Darnet, UFPA / Laboratoire International Associe PALMHEAT CNRS/ UFPA, Recherche et Cooperation Internationale (DERCI). |
Título: |
Transcriptional profiling by RNA sequencing of black pepper (Piper nigrum L.) roots infected by Fusarium solani f. sp. piperis. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Acta Physiologiae Plantarum, v. 39, n. 10, article 239, Oct. 2017. |
Idioma: |
Português |
Conteúdo: |
Black pepper (Piper nigrum L.) is the most traded spice worldwide and therefore has great economic value. One of the major limitations of its production is yield losses in fields due to root rot, a disease caused by Fusarium solani f. sp. piperis. This soil-borne pathogen disseminates rapidly in tropical countries. Biotechnological breeding is often presented as an efficient tool to produce resistant pepper cultivars. An RNA-sequencing experiment was used to take a snapshot of the root transcriptome 60 days after infection by the pathogen. The mapping of 67 million SOLiD single-end reads to the Fusarium genome identified many fungal transcripts coding constitutive proteins and two proteins involved in virulence and conidial formation. The annotation of black pepper transcripts obtained by de novo assembly predicted three proteins restricted to this species. While these transcripts were upregulated in infected roots, the corresponding predicted proteins had no hit in databases. A global analysis of differentially expressed black pepper genes, using terms of gene ontology, has demonstrated an enrichment of genes involved in proteolysis, plastid degradation, cell-wall remodeling and secondary metabolism, consistent with toxicity and necrotrophic fungal interactions that force plants to collaborate at the metabolic level. Detailed descriptions of up- or downregulated genes associated with plant defense suggested several genes implied in the biosynthesis of isoprenoids, especially for an upregulated terpene synthase, a putative geranylgeranyl diphosphate synthase, and a downregulated brassinosteroid signaling component (BAK1). These results provide new clues for investigating further target genes and biotechnological approaches for breeding black pepper resistance to Fusarium. MenosBlack pepper (Piper nigrum L.) is the most traded spice worldwide and therefore has great economic value. One of the major limitations of its production is yield losses in fields due to root rot, a disease caused by Fusarium solani f. sp. piperis. This soil-borne pathogen disseminates rapidly in tropical countries. Biotechnological breeding is often presented as an efficient tool to produce resistant pepper cultivars. An RNA-sequencing experiment was used to take a snapshot of the root transcriptome 60 days after infection by the pathogen. The mapping of 67 million SOLiD single-end reads to the Fusarium genome identified many fungal transcripts coding constitutive proteins and two proteins involved in virulence and conidial formation. The annotation of black pepper transcripts obtained by de novo assembly predicted three proteins restricted to this species. While these transcripts were upregulated in infected roots, the corresponding predicted proteins had no hit in databases. A global analysis of differentially expressed black pepper genes, using terms of gene ontology, has demonstrated an enrichment of genes involved in proteolysis, plastid degradation, cell-wall remodeling and secondary metabolism, consistent with toxicity and necrotrophic fungal interactions that force plants to collaborate at the metabolic level. Detailed descriptions of up- or downregulated genes associated with plant defense suggested several genes implied in the biosynthesis of isoprenoids, especiall... Mostrar Tudo |
Palavras-Chave: |
Melhoramento genético. |
Thesagro: |
Doença; Pimenta. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02668naa a2200289 a 4500 001 2076203 005 2021-12-22 008 2017 bl uuuu u00u1 u #d 100 1 $aMOREIRA, E. C. O. 245 $aTranscriptional profiling by RNA sequencing of black pepper (Piper nigrum L.) roots infected by Fusarium solani f. sp. piperis.$h[electronic resource] 260 $c2017 520 $aBlack pepper (Piper nigrum L.) is the most traded spice worldwide and therefore has great economic value. One of the major limitations of its production is yield losses in fields due to root rot, a disease caused by Fusarium solani f. sp. piperis. This soil-borne pathogen disseminates rapidly in tropical countries. Biotechnological breeding is often presented as an efficient tool to produce resistant pepper cultivars. An RNA-sequencing experiment was used to take a snapshot of the root transcriptome 60 days after infection by the pathogen. The mapping of 67 million SOLiD single-end reads to the Fusarium genome identified many fungal transcripts coding constitutive proteins and two proteins involved in virulence and conidial formation. The annotation of black pepper transcripts obtained by de novo assembly predicted three proteins restricted to this species. While these transcripts were upregulated in infected roots, the corresponding predicted proteins had no hit in databases. A global analysis of differentially expressed black pepper genes, using terms of gene ontology, has demonstrated an enrichment of genes involved in proteolysis, plastid degradation, cell-wall remodeling and secondary metabolism, consistent with toxicity and necrotrophic fungal interactions that force plants to collaborate at the metabolic level. Detailed descriptions of up- or downregulated genes associated with plant defense suggested several genes implied in the biosynthesis of isoprenoids, especially for an upregulated terpene synthase, a putative geranylgeranyl diphosphate synthase, and a downregulated brassinosteroid signaling component (BAK1). These results provide new clues for investigating further target genes and biotechnological approaches for breeding black pepper resistance to Fusarium. 650 $aDoença 650 $aPimenta 653 $aMelhoramento genético 700 1 $aPINHEIRO, D. G. 700 1 $aGORDO, S. M. C. 700 1 $aRODRIGUES, S. de M. 700 1 $aPESSOA, E. 700 1 $aSCHALLER, H. 700 1 $aLEMOS, O. F. de 700 1 $aSILVA, A. 700 1 $aSCHNEIDER, H. 700 1 $aSILVA JUNIOR, W. A. 700 1 $aSAMPAIO, I. 700 1 $aDARNET, S. 773 $tActa Physiologiae Plantarum$gv. 39, n. 10, article 239, Oct. 2017.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Amazônia Oriental (CPATU) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registros recuperados : 7 | |
2. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | GORDO, S. M. da C.; MOREIRA, E. C. de O.; SCHNEIDER, H.; DARNET, S.; RODRIGUES, S. Sequenciamento do transcriptoma de Pimenta-do-reino (Piper nigrum) utilizando Plataforma de Sequenciadores de Nova Geração (NGS) - SOLiD System 3 Plus. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. 1 CD-ROM.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Amazônia Oriental. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso ao objeto digital](/consulta/web/img/pdf.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
4. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | NOGUEIRA, F.; RÊGO, P. S. do; QUEIROZ, H.; VENERE, P.; VARELA, E. S.; SAMPAIO, I.; SCHNEIDER, H.; ARARIPE, J. Genetic diversity and structuring in the arapaima (Osteoglossiformes, Osteoglossidae) population reveal differences between the Amazon and the Tocantins-Araguaia basins. Anais da Academia Brasileira de Ciências, v. 92, n. 1, e20180496, mar. 2020.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Pesca e Aquicultura. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso ao objeto digital](/consulta/web/img/pdf.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
5. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MOREIRA, E. C. O.; PINHEIRO, D. G.; GORDO, S. M. C.; RODRIGUES, S. de M.; PESSOA, E.; SCHALLER, H.; LEMOS, O. F. de; SILVA, A.; SCHNEIDER, H.; SILVA JUNIOR, W. A.; SAMPAIO, I.; DARNET, S. Transcriptional profiling by RNA sequencing of black pepper (Piper nigrum L.) roots infected by Fusarium solani f. sp. piperis. Acta Physiologiae Plantarum, v. 39, n. 10, article 239, Oct. 2017.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Amazônia Oriental. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso restrito ao objeto digital](/consulta/web/img/lock.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
6. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | GORDO, S. M. C.; PINHEIRO, D. G.; MOREIRA, E. C. O.; RODRIGUES, S. M.; POLTRONIERI, M. C.; LEMOS, O. F. de; SILVA, I. T. da; RAMOS, R. T. J.; SILVA, A.; SCHNEIDER, H.; SILVA JUNIOR, W. A.; SAMPAIO, I.; DARNET, S. High-throughput sequencing of black pepper root transcriptome. BMC Plant Biology, v. 12, n. 168, 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Amazônia Oriental. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso ao objeto digital](/consulta/web/img/pdf.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
7. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ANGELO, P. C. da S.; ALMEIDA, E. R. P.; BRÍGIDO, M. de M.; PORTO, J. R.; VITAL, M. J. S.; PEIXOTO, J. C. da C.; SCHNEIDER, M. P. C.; SCHNEIDER, H.; FERNANDEZ, W.; MESQUITA, E. R. L. R. B. P. L.; SILVEIRA, M. A. da; SILVA, L. H. P.; CARVALHO, M. L.; ASTOLFI FILHO, S. Genoma funcional do fruto do guaranazeiro: exame dos resultados da fase de anotação automática. In: PEREIRA, J. C. R.; ARRUDA, M. R. de (Ed.). Pesquisa com guaranazeiro na Embrapa Amazônia Ocidental: status atual e perspectivas. Manaus: Embrapa Amazônia Ocidental, 2007. p. 122-126.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Amazônia Ocidental. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso ao objeto digital](/consulta/web/img/pdf.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
Registros recuperados : 7 | |
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|