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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
06/08/2003 |
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Data da última atualização: |
01/12/2023 |
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Autoria: |
CRUZ, E. T.; HERNÁNDEZ GONZÁLEZ, R.; MARTINEZ RODRIGUEZ, A.; RAMIREZ ÁLVAREZ, H.; ORTEGA, M. E. T.; SCHMID, R. K.; AGUILAR SETIÉN, A. |
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Título: |
Immunoelectrotransference detection of antibodies against caprine artritis encephalitis virus (CAEV). |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Veterinária México, v. 34, n. 2, p. 119-127, 2003. |
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Idioma: |
Inglês |
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Conteúdo: |
Caprine arthritis encephalitis virus (CAEV) is present in Mexico, but its precise prevalence and incidence remains unknown. In order to evaluate the use of immunoelectrotransference or western blot (WB) for the detection of anti-caprine arthritis encephalitis virus antibodies in goats, 50 random serum samples from indigenous animals were analyzed (25 from Amazcala, Queretaro and 25 from Cuatitlan, State of Mexico), and compared with the ELISA. Animals were considered positive for WB when at least a single protein was codified by the gag gene simultaneously with another gene: env/pol; the same criteria used for HIV/AIDS detection. Five antigen - antibody bands (12, 14,25, 50 and 135 kDa) were considered reliable diagnostic markers. When ELISA was used, 33 sera were positive, and 17 were negative. A1l33 ELISA - positive sera were a1so WB positive. at the 17 negative ELISA - sera samples, nine were positive and eight were negative by WB. There is a significant correlation between ELISA and WB serological tests (P < 0.0003). The WB had greater sensitivity than ELISA since it detected eight more positive Sera. These results indicate that WB is a useful tool for confirmatory diagnosis of CAEV, as has been the case with VIH/ AIDS in Mexico. |
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Palavras-Chave: |
Artrite encefalite caprina; CAEV. |
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Thesagro: |
Anticorpo; Caprino; Elisa; Imunofluorescência. |
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Thesaurus Nal: |
Mexico. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02041naa a2200277 a 4500
001 1529485
005 2023-12-01
008 2003 bl uuuu u00u1 u #d
100 1 $aCRUZ, E. T.
245 $aImmunoelectrotransference detection of antibodies against caprine artritis encephalitis virus (CAEV).
260 $c2003
520 $aCaprine arthritis encephalitis virus (CAEV) is present in Mexico, but its precise prevalence and incidence remains unknown. In order to evaluate the use of immunoelectrotransference or western blot (WB) for the detection of anti-caprine arthritis encephalitis virus antibodies in goats, 50 random serum samples from indigenous animals were analyzed (25 from Amazcala, Queretaro and 25 from Cuatitlan, State of Mexico), and compared with the ELISA. Animals were considered positive for WB when at least a single protein was codified by the gag gene simultaneously with another gene: env/pol; the same criteria used for HIV/AIDS detection. Five antigen - antibody bands (12, 14,25, 50 and 135 kDa) were considered reliable diagnostic markers. When ELISA was used, 33 sera were positive, and 17 were negative. A1l33 ELISA - positive sera were a1so WB positive. at the 17 negative ELISA - sera samples, nine were positive and eight were negative by WB. There is a significant correlation between ELISA and WB serological tests (P < 0.0003). The WB had greater sensitivity than ELISA since it detected eight more positive Sera. These results indicate that WB is a useful tool for confirmatory diagnosis of CAEV, as has been the case with VIH/ AIDS in Mexico.
650 $aMexico
650 $aAnticorpo
650 $aCaprino
650 $aElisa
650 $aImunofluorescência
653 $aArtrite encefalite caprina
653 $aCAEV
700 1 $aHERNÁNDEZ GONZÁLEZ, R.
700 1 $aMARTINEZ RODRIGUEZ, A.
700 1 $aRAMIREZ ÁLVAREZ, H.
700 1 $aORTEGA, M. E. T.
700 1 $aSCHMID, R. K.
700 1 $aAGUILAR SETIÉN, A.
773 $tVeterinária México$gv. 34, n. 2, p. 119-127, 2003.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| 1. |  | ZUFFA, S.; SCHMID, R.; BAUERMEISTER, A.; GOMES, P. W. P.; CARABALLO-RODRIGUEZ, A. M.; EL ABIEAD, Y.; ARON, A. T.; GENTRY, E. C.; ZEMLIN, J.; MEEHAN, M. J.; AVALON, N. E.; CICHEWICZ, R. H.; BUZUN, E.; CARRILLO TERRAZAS, M.; HSU, C. Y.; OLES, R.; AYALA, A. V.; ZHAO, J.; CHU, H.; KUIJPERS, M. C. M.; JACKREL, S. L.; TUGIZIMANA, F.; NEPHALI, L. P.; DUBERY, I. A.; MADALA, N. E.; MOREIRA, E. A.; COSTA-LOTUFO, L. V.; LOPES, N. P.; REZENDE-TEIXEIRA, P.; JIMENEZ, P. C.; RIMAL, B.; PATTERSON, A. D.; TRAXLER, M. F.; PESSOTTI, R. de C.; ALVARADO-VILLALOBOS, D.; TAMAYO-CASTILLO, G.; CHAVERRI, P.; ESCUDERO-LEYVA, E.; QUIROS-GUERRERO, L. M.; BORY, A. J.; JOUBERT, J.; RUTZ, A.; WOLFENDER, J. L.; ALLARD, P. M.; SICHERT, A.; PONTRELLI, S.; PULLMAN, B. S.; BANDEIRA, N.; GERWICK, W. H.; GINDRO, K.; MASSANA-CODINA, J.; WAGNER, B. C.; FORCHHAMMER, K.; PETRAS, D.; AIOSA, N.; GARG, N.; LIEBEKE, M.; BOURCEAU, P.; KANG, K. B.; GADHAVI, H.; CARVALHO, L. P. S. de; SANTOS, M. S. dos; PÉREZ-LORENTE, A. I.; MOLINA-SANTIAGO, C.; ROMERO, D.; FRANKE, R.; BRÖNSTRUP, M.; PONCE DE LEÓN, A. V.; POPE, P. B.; LA ROSA, S. L.; LA BARBERA, G.; ROAGER, H. M.; LAURSEN, M. F.; HAMMERLE, F.; SIEWERT, B.; PEINTNER, U.; LICONA-CASSANI, C.; RODRIGUEZ-ORDUÑA, L.; RAMPLER, E.; HILDEBRAND, F.; KOELLENSPERGER, G.; SCHOENY, H.; HOHENWALLNER, K.; PANZENBOECK, L.; GREGOR, R.; O'NEILL, E. C.; ROXBOROUGH, E. T.; ODOI, J.; BALE, N. J.; DING, S.; DAMSTÉ, J. S. S.; GUAN, X. L.; CUI, J. J.; JU, K. S.; SILVA, D. B.; SILVA, F. M. R.; SILVA, G. F. da; KOOLEN, H. H. F.; GRUNDMANN, C.; CLEMENT, J. A.; MOHIMANI, H.; BRODERS, K.; McPHAIL, K. L.; OBER-SINGLETON, S. E.; RATH, C. M.; McDONALD, D.; KNIGHT, R.; WANG, M.; DORRESTEIN, P. C. MicrobeMASST: a taxonomically informed mass spectrometry search tool for microbial metabolomics data. Nature Microbiology, v. 9, p. 336-345, Feb. 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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