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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
30/12/2019 |
Data da última atualização: |
06/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SBARDELLA, A. P.; WELLER, M. M. D. C. A.; I. FONSECA; N. B. STAFUZZA; P. A. BERNARDES; F. F. e SILVA; SILVA, M. V. G. B.; MARTINS, M. F.; D. P. MUNARI. |
Afiliação: |
A. P. SBARDELLA; M. M. D. C. A. WELLER; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; MARTA FONSECA MARTINS, CNPGL. |
Título: |
Ribonucleic acid sequencing differential gene expression analysis of isolated perfused bovine udders experimentally inoculated with Streptococcus agalactiae. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Journal of Dairy Science, v. 102, n. 2, p. 1761-1767, 2019. |
DOI: |
https://doi.org/10.3168/jds.2018-15516 |
Idioma: |
Inglês |
Conteúdo: |
The aim of this study was to elucidate the differential gene expression in the RNA sequencing transcriptome of isolated perfused udders collected from 4 slaughtered Holstein × Zebu crossbred dairy cows experimentally inoculated with Streptococcus agalactiae. We studied 3 different statistical tools (edgeR, baySeq, and Cuffdiff 2). In summary, 2 quarters of each udder were experimentally inoculated with Strep. agalactiae and the other 2 were used as a control. Mammary tissue biopsies were collected at times 0 and 3 h after infection. The total RNA was extracted and sequenced on an Illumina HiSeq 2000 (Illumina Inc., San Diego, CA). Transcripts were assembled from the reads aligned to the bovine UMD 3.1 reference genome, and the statistical analyses were performed using the previously mentioned tools (edgeR, baySeq, and Cuffdiff 2). Finally, the identified genes were submitted to pathway enrichment analysis. A total of 1,756, 1,161, and 3,389 genes with differential gene expression were identified when using edgeR, baySeq, and Cuffdiff 2, respectively. A total of 122 genes were identified by the overlapping of the 3 methods; however, only the platelet activation presented a significantly enriched pathway. From the results, we suggest the FCER1G, GNAI2, ORAI1, and VASP genes shared among the 3 methods in this pathway for posterior biological validation. |
Palavras-Chave: |
BaySeq; Crossbred dairy cow; Cuffdiff 2; EdgeR; RNA sequencing. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02292naa a2200289 a 4500 001 2117886 005 2024-02-06 008 2019 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3168/jds.2018-15516$2DOI 100 1 $aSBARDELLA, A. P. 245 $aRibonucleic acid sequencing differential gene expression analysis of isolated perfused bovine udders experimentally inoculated with Streptococcus agalactiae.$h[electronic resource] 260 $c2019 520 $aThe aim of this study was to elucidate the differential gene expression in the RNA sequencing transcriptome of isolated perfused udders collected from 4 slaughtered Holstein × Zebu crossbred dairy cows experimentally inoculated with Streptococcus agalactiae. We studied 3 different statistical tools (edgeR, baySeq, and Cuffdiff 2). In summary, 2 quarters of each udder were experimentally inoculated with Strep. agalactiae and the other 2 were used as a control. Mammary tissue biopsies were collected at times 0 and 3 h after infection. The total RNA was extracted and sequenced on an Illumina HiSeq 2000 (Illumina Inc., San Diego, CA). Transcripts were assembled from the reads aligned to the bovine UMD 3.1 reference genome, and the statistical analyses were performed using the previously mentioned tools (edgeR, baySeq, and Cuffdiff 2). Finally, the identified genes were submitted to pathway enrichment analysis. A total of 1,756, 1,161, and 3,389 genes with differential gene expression were identified when using edgeR, baySeq, and Cuffdiff 2, respectively. A total of 122 genes were identified by the overlapping of the 3 methods; however, only the platelet activation presented a significantly enriched pathway. From the results, we suggest the FCER1G, GNAI2, ORAI1, and VASP genes shared among the 3 methods in this pathway for posterior biological validation. 653 $aBaySeq 653 $aCrossbred dairy cow 653 $aCuffdiff 2 653 $aEdgeR 653 $aRNA sequencing 700 1 $aWELLER, M. M. D. C. A. 700 1 $aI. FONSECA 700 1 $aN. B. STAFUZZA 700 1 $aP. A. BERNARDES 700 1 $aF. F. e SILVA 700 1 $aSILVA, M. V. G. B. 700 1 $aMARTINS, M. F. 700 1 $aD. P. MUNARI 773 $tJournal of Dairy Science$gv. 102, n. 2, p. 1761-1767, 2019.
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Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
23/11/2022 |
Data da última atualização: |
24/11/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
ROMERO PELÁEZ, R. D.; WISCHRAL, D.; CUNHA, J. R. B.; MENDES, T. D.; PACHECO, T. F.; SIQUEIRA, F. G. de; ALMEIDA, J. R. M. de. |
Afiliação: |
RUBÉN DARÍO ROMERO PELÁEZ, UNIVERSIDADE DE BRASÍLIA; DAIANA WISCHRAL, CNPAE; JOICE RAÍSA BARBOSA CUNHA, CNPAE; THAIS DEMARCHI MENDES, CNPAE; THALYTA FRAGA PACHECO, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE. |
Título: |
Production of enzymatic extract with high cellulolytic and oxidative activities by co-culture of Trichoderma reesei and Panus lecomtei. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Fermentation, v. 8, n. 10, 522, 2022. |
Páginas: |
17 p. |
ISSN: |
2311-5637 |
DOI: |
https://doi.org/10.3390/fermentation8100522 |
Idioma: |
Inglês |
Conteúdo: |
This work aimed to produce enzymatic fungi extracts with hydrolytic and oxidative activities to hydrolyze lignocellulosic biomasses efficiently. For this, the fungi Trichoderma reesei and Panus lecomtei were co-cultured using the vegetable biomasses oil palm decanter cake, wheat bran, and cottonseed cake as substrates in submerged fermentation. T. reesei and P. lecomtei showed partially compatible positive interaction on plates. The co-cultures respond positively to variations of temperature and inoculum interval, generating extracts responsible for higher hydrolysis yield when grown at 25 °C, and P. lecomtei is inoculated 24 h after T. reesei. The enzymatic extract production of co-cultures was also improved by modifying the components of the initial media and evaluating enzymatic activities, hydrolysis of sugarcane bagasse pretreated by autohydrolysis and ethanol production as a response. Five culture media were evaluated with variations in the composition of nutritional elements, minerals and substrates. The best extract showed a maximum cellulose hydrolysis efficiency of 68.7% compared with 44.8% of the initial medium. The ethanolic fermentation of hydrolysates obtained by co-culture extracts showed higher ethanol yields than monocultures. This work demonstrates the use of fungi co-cultures to produce enzymatic extracts composed of cellulolytic, hemicellulolytic, and ligninolytic enzymes complexes, which allow hydrolyzing pretreated lignocellulosic biomass with high efficiency, generating hydrolysates that are easier fermented by yeast. MenosThis work aimed to produce enzymatic fungi extracts with hydrolytic and oxidative activities to hydrolyze lignocellulosic biomasses efficiently. For this, the fungi Trichoderma reesei and Panus lecomtei were co-cultured using the vegetable biomasses oil palm decanter cake, wheat bran, and cottonseed cake as substrates in submerged fermentation. T. reesei and P. lecomtei showed partially compatible positive interaction on plates. The co-cultures respond positively to variations of temperature and inoculum interval, generating extracts responsible for higher hydrolysis yield when grown at 25 °C, and P. lecomtei is inoculated 24 h after T. reesei. The enzymatic extract production of co-cultures was also improved by modifying the components of the initial media and evaluating enzymatic activities, hydrolysis of sugarcane bagasse pretreated by autohydrolysis and ethanol production as a response. Five culture media were evaluated with variations in the composition of nutritional elements, minerals and substrates. The best extract showed a maximum cellulose hydrolysis efficiency of 68.7% compared with 44.8% of the initial medium. The ethanolic fermentation of hydrolysates obtained by co-culture extracts showed higher ethanol yields than monocultures. This work demonstrates the use of fungi co-cultures to produce enzymatic extracts composed of cellulolytic, hemicellulolytic, and ligninolytic enzymes complexes, which allow hydrolyzing pretreated lignocellulosic biomass with high effi... Mostrar Tudo |
Palavras-Chave: |
Co-cultures; Ethanolic fermentation; Laccases; Panus lecomtei. |
Thesaurus NAL: |
Cellulases; Trichoderma reesei. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148635/1/Production-of-enzymatic-extract.pdf
|
Marc: |
LEADER 02486naa a2200301 a 4500 001 2148635 005 2022-11-24 008 2022 bl uuuu u00u1 u #d 022 $a2311-5637 024 7 $ahttps://doi.org/10.3390/fermentation8100522$2DOI 100 1 $aROMERO PELÁEZ, R. D. 245 $aProduction of enzymatic extract with high cellulolytic and oxidative activities by co-culture of Trichoderma reesei and Panus lecomtei.$h[electronic resource] 260 $c2022 300 $a17 p. 520 $aThis work aimed to produce enzymatic fungi extracts with hydrolytic and oxidative activities to hydrolyze lignocellulosic biomasses efficiently. For this, the fungi Trichoderma reesei and Panus lecomtei were co-cultured using the vegetable biomasses oil palm decanter cake, wheat bran, and cottonseed cake as substrates in submerged fermentation. T. reesei and P. lecomtei showed partially compatible positive interaction on plates. The co-cultures respond positively to variations of temperature and inoculum interval, generating extracts responsible for higher hydrolysis yield when grown at 25 °C, and P. lecomtei is inoculated 24 h after T. reesei. The enzymatic extract production of co-cultures was also improved by modifying the components of the initial media and evaluating enzymatic activities, hydrolysis of sugarcane bagasse pretreated by autohydrolysis and ethanol production as a response. Five culture media were evaluated with variations in the composition of nutritional elements, minerals and substrates. The best extract showed a maximum cellulose hydrolysis efficiency of 68.7% compared with 44.8% of the initial medium. The ethanolic fermentation of hydrolysates obtained by co-culture extracts showed higher ethanol yields than monocultures. This work demonstrates the use of fungi co-cultures to produce enzymatic extracts composed of cellulolytic, hemicellulolytic, and ligninolytic enzymes complexes, which allow hydrolyzing pretreated lignocellulosic biomass with high efficiency, generating hydrolysates that are easier fermented by yeast. 650 $aCellulases 650 $aTrichoderma reesei 653 $aCo-cultures 653 $aEthanolic fermentation 653 $aLaccases 653 $aPanus lecomtei 700 1 $aWISCHRAL, D. 700 1 $aCUNHA, J. R. B. 700 1 $aMENDES, T. D. 700 1 $aPACHECO, T. F. 700 1 $aSIQUEIRA, F. G. de 700 1 $aALMEIDA, J. R. M. de 773 $tFermentation$gv. 8, n. 10, 522, 2022.
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