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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Suínos e Aves. |
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Data corrente: |
09/03/2023 |
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Data da última atualização: |
09/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, A. N. da; IBELLI, A. M. G.; SAVOLDI, I. R.; CANTAO, M. E.; ZANELLA, E. L.; MARQUES, M. G.; SILVA, M. V. G. B.; PEIXOTO, J. de O.; LEDUR, M. C.; LOPES, J. S.; VARGAS, J. E.; ZANELLA, R. |
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Afiliação: |
ARTHUR NERY DA SILVA, Universidade de Passo Fundo; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; IGOR RICARDO SAVOLDI, Universidade do Contestado/Concórdia; MAURICIO EGIDIO CANTAO, CNPSA; ERALDO LOURENSO ZANELLA, UNIVERSIDADE; MARIANA GROKE MARQUES, CNPSA; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; JANE DE OLIVEIRA PEIXOTO, CNPSA; MONICA CORREA LEDUR, CNPSA; JADER SILVA LOPES, BRF/Curitiba; JOSÉ EDUARDO VARGAS, Universidade Federal do Paraná; RICARDO ZANELLA, Universidade de Passo Fundo. |
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Título: |
Whole-exome sequencing indicated new candidate genes associated with unilateral cryptorchidism in pigs. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Sexual Development, 9 Feb 2023. |
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DOI: |
10.1159/000528360 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract:
Introduction: Cryptorchidism is a hereditary anomaly characterized by the incomplete descent of one or both testicles to the scrotum. One of the challenges of this anomaly is that the retained testicle maintains its endocrine function. As a consequence, cryptorchid animals produce hormone-tainted meat in comparison to castrated animals and are likely to be more aggressive. Cryptorchidism can lead to reduced animal welfare outcomes and cause economic losses. Identifying genetic markers for cryptorchidism is an essential step toward mitigating these negative outcomes and may facilitate genome manipulation to reduce the occurrence of cryptorchidism. Attempts to identify such markers have used genome-wide association studies. Using whole-exome sequencing, we aimed to identify single nucleotide polymorphisms (SNPs) in the coding regions of cryptorchid pigs and to characterize functional pathways concerning these SNPs.
Methods: DNA was extracted and sequenced from 5 healthy and 5 cryptorchid animals from the Landrace breed, using the Illumina HiSeq 2500 platform. Data were pre-processed using the SeqyClean tool and further mapped against the swine reference genome (Sus scrofa 11.1) using BWA software. GATK was used to identify polymorphisms (SNPs and InDels), which were annotated using the VEP tool. Network prediction and gene ontology enrichment analysis were conducted using the Cytoscape platform, and STRING software was used for visualization.
Results: A total of 63 SNPs were identified across the genes PIGB, CCPG1, COMMD9, LDLRAD3, TRIM44, MYLPF, SEPTIN, ZNF48, TIA1, FAIM2, KRT18, FBP1, FBP2, CTSL, DAPK1, DHX8, GPR179, DEPDC1B, ENSSSCG00000049573, ENSSSCG00000016384, ENSSSCG00000022657, ENSSSCG00000038825, and ENSSSCG00000001229. Using pathway enrichment analyses and network prospection, we have identified the following significant adjusted p value threshold of 0.001 involved with the biological function pathways of estrogen signaling, cytoskeleton organization, and the pentose phosphate pathway.
Conclusion: Our data suggest the involvement of new SNPs and genes in developing cryptorchidism in pigs. However, further studies are needed to validate our results in a larger cohort population. Variations in the GPR179 gene, with implications at the protein level, may be associated with the appearance of this anomaly in the swine. Finally, we are showing that the estrogen signaling pathway may be involved in the pathophysiological mechanisms of this congenital anomaly as previously reported in GWAS. MenosAbstract:
Introduction: Cryptorchidism is a hereditary anomaly characterized by the incomplete descent of one or both testicles to the scrotum. One of the challenges of this anomaly is that the retained testicle maintains its endocrine function. As a consequence, cryptorchid animals produce hormone-tainted meat in comparison to castrated animals and are likely to be more aggressive. Cryptorchidism can lead to reduced animal welfare outcomes and cause economic losses. Identifying genetic markers for cryptorchidism is an essential step toward mitigating these negative outcomes and may facilitate genome manipulation to reduce the occurrence of cryptorchidism. Attempts to identify such markers have used genome-wide association studies. Using whole-exome sequencing, we aimed to identify single nucleotide polymorphisms (SNPs) in the coding regions of cryptorchid pigs and to characterize functional pathways concerning these SNPs.
Methods: DNA was extracted and sequenced from 5 healthy and 5 cryptorchid animals from the Landrace breed, using the Illumina HiSeq 2500 platform. Data were pre-processed using the SeqyClean tool and further mapped against the swine reference genome (Sus scrofa 11.1) using BWA software. GATK was used to identify polymorphisms (SNPs and InDels), which were annotated using the VEP tool. Network prediction and gene ontology enrichment analysis were conducted using the Cytoscape platform, and STRING software was used for visualization.
Results: A total of 63 ... Mostrar Tudo |
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Palavras-Chave: |
Criptorquidismo; Sequenciamento completo do exoma; Whole-Exome Sequencing. |
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Thesagro: |
Gene Marcador; Genética Animal; Marcador Genético; Porco; Suíno. |
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Thesaurus Nal: |
Cryptorchidism. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03604naa a2200373 a 4500
001 2152220
005 2023-03-09
008 2023 bl uuuu u00u1 u #d
024 7 $a10.1159/000528360$2DOI
100 1 $aSILVA, A. N. da
245 $aWhole-exome sequencing indicated new candidate genes associated with unilateral cryptorchidism in pigs.$h[electronic resource]
260 $c2023
520 $aAbstract: Introduction: Cryptorchidism is a hereditary anomaly characterized by the incomplete descent of one or both testicles to the scrotum. One of the challenges of this anomaly is that the retained testicle maintains its endocrine function. As a consequence, cryptorchid animals produce hormone-tainted meat in comparison to castrated animals and are likely to be more aggressive. Cryptorchidism can lead to reduced animal welfare outcomes and cause economic losses. Identifying genetic markers for cryptorchidism is an essential step toward mitigating these negative outcomes and may facilitate genome manipulation to reduce the occurrence of cryptorchidism. Attempts to identify such markers have used genome-wide association studies. Using whole-exome sequencing, we aimed to identify single nucleotide polymorphisms (SNPs) in the coding regions of cryptorchid pigs and to characterize functional pathways concerning these SNPs. Methods: DNA was extracted and sequenced from 5 healthy and 5 cryptorchid animals from the Landrace breed, using the Illumina HiSeq 2500 platform. Data were pre-processed using the SeqyClean tool and further mapped against the swine reference genome (Sus scrofa 11.1) using BWA software. GATK was used to identify polymorphisms (SNPs and InDels), which were annotated using the VEP tool. Network prediction and gene ontology enrichment analysis were conducted using the Cytoscape platform, and STRING software was used for visualization. Results: A total of 63 SNPs were identified across the genes PIGB, CCPG1, COMMD9, LDLRAD3, TRIM44, MYLPF, SEPTIN, ZNF48, TIA1, FAIM2, KRT18, FBP1, FBP2, CTSL, DAPK1, DHX8, GPR179, DEPDC1B, ENSSSCG00000049573, ENSSSCG00000016384, ENSSSCG00000022657, ENSSSCG00000038825, and ENSSSCG00000001229. Using pathway enrichment analyses and network prospection, we have identified the following significant adjusted p value threshold of 0.001 involved with the biological function pathways of estrogen signaling, cytoskeleton organization, and the pentose phosphate pathway. Conclusion: Our data suggest the involvement of new SNPs and genes in developing cryptorchidism in pigs. However, further studies are needed to validate our results in a larger cohort population. Variations in the GPR179 gene, with implications at the protein level, may be associated with the appearance of this anomaly in the swine. Finally, we are showing that the estrogen signaling pathway may be involved in the pathophysiological mechanisms of this congenital anomaly as previously reported in GWAS.
650 $aCryptorchidism
650 $aGene Marcador
650 $aGenética Animal
650 $aMarcador Genético
650 $aPorco
650 $aSuíno
653 $aCriptorquidismo
653 $aSequenciamento completo do exoma
653 $aWhole-Exome Sequencing
700 1 $aIBELLI, A. M. G.
700 1 $aSAVOLDI, I. R.
700 1 $aCANTAO, M. E.
700 1 $aZANELLA, E. L.
700 1 $aMARQUES, M. G.
700 1 $aSILVA, M. V. G. B.
700 1 $aPEIXOTO, J. de O.
700 1 $aLEDUR, M. C.
700 1 $aLOPES, J. S.
700 1 $aVARGAS, J. E.
700 1 $aZANELLA, R.
773 $tSexual Development, 9 Feb 2023.
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Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
10/07/1996 |
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Data da última atualização: |
14/11/2006 |
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Autoria: |
SACRAMENTO, C. K. do; COELHO, Y. da S.; AIDAR, T. |
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Título: |
Maturação e qualidade da tangerina 'Ponkan' e tangor 'Murcott' no Sudeste da Bahia. |
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Ano de publicação: |
1989 |
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Fonte/Imprenta: |
Ilhéus: CEPLAC, 1989. |
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Páginas: |
n.p. |
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Série: |
(CEPLAC. Boletim Técnico, 166). |
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Idioma: |
Português |
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Conteúdo: |
Foram analisados quinzenalmente frutos de tangerina 'Pokan' e tangor 'Murcott' cultivados em Barrolândia, município de Belmonte-BA, objetivando conhecer as suas características físicas e químicas, além de identificar a época ideal para colheita. Os parâmetros analisados foram: peso médio, tamanho, espessura de casca, rendimento em suco, acidez total, sólidos solúveis totais e relação sólidos solúveis totais/acidez. Os resultados obtidos demonstraram que o período ótimo para colheita da tangerina 'Ponkan' ficou reduzido a apenas um mês, enquanto o tangor 'Murcott' permaneceu com as características desejáveis por período mais longo, atendendo as exigências do mercado in natura e da industrialização. |
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Palavras-Chave: |
'Murcott'; 'Pokan'; Características; Citro; Citros; Cultivares; Maturation; Quality; Tangerine. |
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Thesagro: |
Colheita; Maturação; Qualidade; Tangerina. |
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Thesaurus NAL: |
Citrus; harvesting. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01562nam a2200337 a 4500
001 1653803
005 2006-11-14
008 1989 bl uuuu u0uu1 u #d
100 1 $aSACRAMENTO, C. K. do
245 $aMaturação e qualidade da tangerina 'Ponkan' e tangor 'Murcott' no Sudeste da Bahia.
260 $aIlhéus: CEPLAC$c1989
300 $an.p.
490 $a(CEPLAC. Boletim Técnico, 166).
520 $aForam analisados quinzenalmente frutos de tangerina 'Pokan' e tangor 'Murcott' cultivados em Barrolândia, município de Belmonte-BA, objetivando conhecer as suas características físicas e químicas, além de identificar a época ideal para colheita. Os parâmetros analisados foram: peso médio, tamanho, espessura de casca, rendimento em suco, acidez total, sólidos solúveis totais e relação sólidos solúveis totais/acidez. Os resultados obtidos demonstraram que o período ótimo para colheita da tangerina 'Ponkan' ficou reduzido a apenas um mês, enquanto o tangor 'Murcott' permaneceu com as características desejáveis por período mais longo, atendendo as exigências do mercado in natura e da industrialização.
650 $aCitrus
650 $aharvesting
650 $aColheita
650 $aMaturação
650 $aQualidade
650 $aTangerina
653 $a'Murcott'
653 $a'Pokan'
653 $aCaracterísticas
653 $aCitro
653 $aCitros
653 $aCultivares
653 $aMaturation
653 $aQuality
653 $aTangerine
700 1 $aCOELHO, Y. da S.
700 1 $aAIDAR, T.
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