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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
27/04/2010 |
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Data da última atualização: |
28/04/2010 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CUNHA, R. C.; LEITE, F. P. L.; PINTO, L. da S.; SATO, T.; ANDREOTTI, R. |
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Afiliação: |
RODRIGO CASQUERO CUNHA, UFPel; FÁBIO PEREIRA LEIVAS LEITE, UFPel; LUCIANO DA SILVA PINTO, UFPel; TAYRA SATO, UFPel; RENATO ANDREOTTI E SILVA, CNPGC. |
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Título: |
Subclonagem do gene Bm86-CG em pPIC9 para expressão heteróloga em Pichia pastoris. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE PARASITOLOGIA 21.; ENCONTRO DE PARASITOLOGIA DO MERCOSUL, 2., 2009, Foz do Iguaçu. Novos horizontes em parasitologia: anais. Foz do Iguaçu: SBP, 2009. p. 1298. Revista Patologia Tropical, v. 38, jul.-set 2009. Suplemento 2. |
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Idioma: |
Português |
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Conteúdo: |
O objetivo deste trabalho foi de subclonar o gene da proteína Bm86-CG, previamente isolado e clonado no laboratório de Biologia Molecular 2A da Embrapa Gado de Corte - MS, em um vetor de expressão eucariótico utilizando a levedura metilotrófica Pichia pastoris. |
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Palavras-Chave: |
Levedura; Rhipicephalus boophilus microplus. |
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Thesagro: |
Carrapato; Clonagem; Sanidade Animal; Vetor. |
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Thesaurus Nal: |
Pichia pastoris. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 01157naa a2200253 a 4500
001 1737003
005 2010-04-28
008 2009 bl --- 0-- u #d
100 1 $aCUNHA, R. C.
245 $aSubclonagem do gene Bm86-CG em pPIC9 para expressão heteróloga em Pichia pastoris.
260 $c2009
520 $aO objetivo deste trabalho foi de subclonar o gene da proteína Bm86-CG, previamente isolado e clonado no laboratório de Biologia Molecular 2A da Embrapa Gado de Corte - MS, em um vetor de expressão eucariótico utilizando a levedura metilotrófica Pichia pastoris.
650 $aPichia pastoris
650 $aCarrapato
650 $aClonagem
650 $aSanidade Animal
650 $aVetor
653 $aLevedura
653 $aRhipicephalus boophilus microplus
700 1 $aLEITE, F. P. L.
700 1 $aPINTO, L. da S.
700 1 $aSATO, T.
700 1 $aANDREOTTI, R.
773 $tIn: CONGRESSO BRASILEIRO DE PARASITOLOGIA 21.; ENCONTRO DE PARASITOLOGIA DO MERCOSUL, 2., 2009, Foz do Iguaçu. Novos horizontes em parasitologia: anais. Foz do Iguaçu: SBP, 2009. p. 1298. Revista Patologia Tropical$gv. 38, jul.-set 2009. Suplemento 2.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
12/07/2022 |
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Data da última atualização: |
03/01/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BRANDÃO, M. da S.; MUNIZ, F.; HERNÁNDEZ-RANGEL, S.; CAMPOS, Z.; FARIAS, I.; HRBEK, T. |
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Afiliação: |
MAYNARA DA SILVA BRANDÃO, Federal University of Amazonas, UFAM, Manaus; FÁBIO MUNIZ, Federal University of Amazonas, UFAM, Manaus; SANDRA HERNÁNDEZ-RANGEL, Federal University of Amazonas, UFAM, Manaus; ZILCA MARIA DA SILVA CAMPOS, CPAP; IZENI FARIAS, Federal University of Amazonas, UFAM, Manaus; TOMAS HRBEK, Federal University of Amazonas, UFAM, Manaus. |
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Título: |
SNPs marker toolbox for molecular identification of nominal and candidate species of the genus Paleosuchus. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
In: WORKING MEETING OF THE CROCODILE SPECIALIST GROUP OF THE INTERNATIONAL UNION FOR CONSERVATION NATURE, 26., 2022, Chetumal. Abstracts... [Chetumal]: CSG, 2022. |
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Páginas: |
p. 123. |
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Idioma: |
Inglês |
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Notas: |
Evento Online. |
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Conteúdo: |
Recent studies have identified the existence of species complexes in crocodilians, including in the New World Caimaninae. Both species of the genus Paleosuchus are species complexes with three or four species subsumed under the P. palpebrosus epithet and two under the P. trigonatus epithet. From the conservation point of view, it is important to recognize the evolutionary and ecological uniqueness of these lineages, regardless of the taxonomic status they may receive. Candidate species of the genus Paleosuchus have not yet been formally described and therefore do not have diagnostic morphological characters that would allow their identification in nature. This problem motivated us to develop a SNP (Single Nucleotide Polymorphisms) toolkit that enables molecular identification of the nominal and candidate species of Paleosuchus. For this, a ddRADseq (Double Digest RAD-sequencing) protocol was used to obtain reduced representation genome of 20 P. palpebrosus samples, five from each of four lineages ('Amazonia', 'Bolivia', 'Madeira' and 'Pantanal'), and five samples of P. trigonatus, three from the 'Amazon' and two from the 'Guyana' lineage. We selected from between 11 and 15 private SNPs for each lineage that unambiguously assign individuals to one of these lineages. Additionally, we selected 11 mutually exclusive private SNPs that unambiguously assign individuals to one of the two species complexes. The use of the toolkit is affordable and fast-to-apply in comparison to other molecular identification methods, and so it will enable a rapid molecular assignment of individuals to the newly identified lineages of the genus, which may be suffering different degrees of threat. Additional applications of this marker set are: the assignment of zoo specimens, pet trade individuals, museum samples, or degraded samples to source lineages; molecular identification seized animal parts (eg. bush meat); and identification of the origin of illegally traded animals or their by-products (forensic genetics). MenosRecent studies have identified the existence of species complexes in crocodilians, including in the New World Caimaninae. Both species of the genus Paleosuchus are species complexes with three or four species subsumed under the P. palpebrosus epithet and two under the P. trigonatus epithet. From the conservation point of view, it is important to recognize the evolutionary and ecological uniqueness of these lineages, regardless of the taxonomic status they may receive. Candidate species of the genus Paleosuchus have not yet been formally described and therefore do not have diagnostic morphological characters that would allow their identification in nature. This problem motivated us to develop a SNP (Single Nucleotide Polymorphisms) toolkit that enables molecular identification of the nominal and candidate species of Paleosuchus. For this, a ddRADseq (Double Digest RAD-sequencing) protocol was used to obtain reduced representation genome of 20 P. palpebrosus samples, five from each of four lineages ('Amazonia', 'Bolivia', 'Madeira' and 'Pantanal'), and five samples of P. trigonatus, three from the 'Amazon' and two from the 'Guyana' lineage. We selected from between 11 and 15 private SNPs for each lineage that unambiguously assign individuals to one of these lineages. Additionally, we selected 11 mutually exclusive private SNPs that unambiguously assign individuals to one of the two species complexes. The use of the toolkit is affordable and fast-to-apply in comparison to other... Mostrar Tudo |
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Thesagro: |
Jacaré. |
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Thesaurus NAL: |
Caiman. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144666/1/CSG26-Abstracts-Program-220707-151117-123.pdf
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Marc: |
LEADER 02768nam a2200217 a 4500
001 2144666
005 2023-01-03
008 2022 bl uuuu u00u1 u #d
100 1 $aBRANDÃO, M. da S.
245 $aSNPs marker toolbox for molecular identification of nominal and candidate species of the genus Paleosuchus.$h[electronic resource]
260 $aIn: WORKING MEETING OF THE CROCODILE SPECIALIST GROUP OF THE INTERNATIONAL UNION FOR CONSERVATION NATURE, 26., 2022, Chetumal. Abstracts... [Chetumal]: CSG$c2022
300 $ap. 123.
500 $aEvento Online.
520 $aRecent studies have identified the existence of species complexes in crocodilians, including in the New World Caimaninae. Both species of the genus Paleosuchus are species complexes with three or four species subsumed under the P. palpebrosus epithet and two under the P. trigonatus epithet. From the conservation point of view, it is important to recognize the evolutionary and ecological uniqueness of these lineages, regardless of the taxonomic status they may receive. Candidate species of the genus Paleosuchus have not yet been formally described and therefore do not have diagnostic morphological characters that would allow their identification in nature. This problem motivated us to develop a SNP (Single Nucleotide Polymorphisms) toolkit that enables molecular identification of the nominal and candidate species of Paleosuchus. For this, a ddRADseq (Double Digest RAD-sequencing) protocol was used to obtain reduced representation genome of 20 P. palpebrosus samples, five from each of four lineages ('Amazonia', 'Bolivia', 'Madeira' and 'Pantanal'), and five samples of P. trigonatus, three from the 'Amazon' and two from the 'Guyana' lineage. We selected from between 11 and 15 private SNPs for each lineage that unambiguously assign individuals to one of these lineages. Additionally, we selected 11 mutually exclusive private SNPs that unambiguously assign individuals to one of the two species complexes. The use of the toolkit is affordable and fast-to-apply in comparison to other molecular identification methods, and so it will enable a rapid molecular assignment of individuals to the newly identified lineages of the genus, which may be suffering different degrees of threat. Additional applications of this marker set are: the assignment of zoo specimens, pet trade individuals, museum samples, or degraded samples to source lineages; molecular identification seized animal parts (eg. bush meat); and identification of the origin of illegally traded animals or their by-products (forensic genetics).
650 $aCaiman
650 $aJacaré
700 1 $aMUNIZ, F.
700 1 $aHERNÁNDEZ-RANGEL, S.
700 1 $aCAMPOS, Z.
700 1 $aFARIAS, I.
700 1 $aHRBEK, T.
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Embrapa Pantanal (CPAP) |
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