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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
Data corrente: |
07/12/2015 |
Data da última atualização: |
26/04/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GUIMARAES, P. M.; GUIMARAES, L. A.; MORGANTE, C. V.; SILVA JUNIOR, O. B.; ARAUJO, A. C. G.; MARTINS, A. C. Q.; SARAIVA, M. A. P.; OLIVEIRA, T. N.; TOGAWA, R. C.; LEAL-BERTIOLI, S. C. M.; BERTIOLI, D. J.; BRASILEIRO, A. C. M. |
Afiliação: |
PATRICIA MESSEMBERG GUIMARAES, CENARGEN; LARISSA A. GUIMARAES; CAROLINA VIANNA MORGANTE, CPATSA; ORZENIL BONFIM DA SILVA JUNIOR, CENARGEN; ANA CLAUDIA GUERRA DE ARAUJO, CENARGEN; ANDRESSA C. Q. MARTINS, UNB; MARIO ALFREDO DE PASSOS SARAIVA, CENARGEN; THAIS N. OLIVEIRA, UNB; ROBERTO COITI TOGAWA, CENARGEN; SORAYA CRISTINA DE M LEAL BERTIOLI, CENARGEN; DAVID J. BERTIOLI, UNB; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN. |
Título: |
Root transcriptome analysis of wild peanut reveals candidate genes for nematode resistance. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Plos One, October 21, p. 1-22, 2015. (Open Access). |
DOI: |
10.1371/journal.pone.0140937 |
Idioma: |
Inglês |
Conteúdo: |
Wild peanut relatives (Arachis spp.) are genetically diverse and were adapted to a range of environments during the evolution course, constituting an important source of allele diver-sity for resistance to biotic and abiotic stresses. The wild diploid A.stenosperma harbors high levels of resistance to a variety of pathogens, including the root-knot nematode (RKN)Meloidogyne arenaria through the onset of the Hypersensitive Response (HR). In order to identify genes and regulators triggering this defense response, a comprehensive root tran-scriptome analysis during the first stages of this incompatible interaction was conducted using Illumina Hi-Seq. Overall, eight cDNA libraries were produced generating 28.2 GB, which were de novo assembled into 44,132 contigs and 37,882 loci. Differentially expressed genes (DEGs) were identified and clustered according to their expression profile, with the majority being downregulated at 6 DAI, which coincides with the onset of the HR. Amongst these DEGs, 27 were selected for further qRT-PCR validation allowing the identifi- cation of nematode-responsive candidate genes that are putatively related to the resistance response. Those candidates are engaged in the salycilic (NBS-LRR, lipocalins, resveratrol synthase) and jasmonic (patatin, allene oxidase cyclase) acids pathways, and also related to hormonal balance (auxin responsive protein, GH3) and cellular plasticity and signaling (tetraspanin, integrin, expansin), with some of them showing contrasting expression behav-ior between Arachis RKN-resistant and susceptible genotypes. As these candidate genes activate different defensive signaling systems, the genetic (HR) and the induced resistance(IR), their pyramidding in one genotype via molecular breeding or transgenic strategy might contribute to a more durable resistance, thus improving the long-term control of RKN in peanut. MenosWild peanut relatives (Arachis spp.) are genetically diverse and were adapted to a range of environments during the evolution course, constituting an important source of allele diver-sity for resistance to biotic and abiotic stresses. The wild diploid A.stenosperma harbors high levels of resistance to a variety of pathogens, including the root-knot nematode (RKN)Meloidogyne arenaria through the onset of the Hypersensitive Response (HR). In order to identify genes and regulators triggering this defense response, a comprehensive root tran-scriptome analysis during the first stages of this incompatible interaction was conducted using Illumina Hi-Seq. Overall, eight cDNA libraries were produced generating 28.2 GB, which were de novo assembled into 44,132 contigs and 37,882 loci. Differentially expressed genes (DEGs) were identified and clustered according to their expression profile, with the majority being downregulated at 6 DAI, which coincides with the onset of the HR. Amongst these DEGs, 27 were selected for further qRT-PCR validation allowing the identifi- cation of nematode-responsive candidate genes that are putatively related to the resistance response. Those candidates are engaged in the salycilic (NBS-LRR, lipocalins, resveratrol synthase) and jasmonic (patatin, allene oxidase cyclase) acids pathways, and also related to hormonal balance (auxin responsive protein, GH3) and cellular plasticity and signaling (tetraspanin, integrin, expansin), with some of them showing co... Mostrar Tudo |
Palavras-Chave: |
Arachis spp; Diplóide selvagem; Gene regulador; Melhoramento de planta; Nematóide das galhas; Peanut; Peanut relatives; Planta resistente. |
Thesagro: |
Arachis Hypogaea. |
Categoria do assunto: |
-- G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/134987/1/pone.0140937.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140762/1/Morgante.pdf
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Marc: |
LEADER 02999naa a2200373 a 4500 001 2030792 005 2024-04-26 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1371/journal.pone.0140937$2DOI 100 1 $aGUIMARAES, P. M. 245 $aRoot transcriptome analysis of wild peanut reveals candidate genes for nematode resistance.$h[electronic resource] 260 $c2015 520 $aWild peanut relatives (Arachis spp.) are genetically diverse and were adapted to a range of environments during the evolution course, constituting an important source of allele diver-sity for resistance to biotic and abiotic stresses. The wild diploid A.stenosperma harbors high levels of resistance to a variety of pathogens, including the root-knot nematode (RKN)Meloidogyne arenaria through the onset of the Hypersensitive Response (HR). In order to identify genes and regulators triggering this defense response, a comprehensive root tran-scriptome analysis during the first stages of this incompatible interaction was conducted using Illumina Hi-Seq. Overall, eight cDNA libraries were produced generating 28.2 GB, which were de novo assembled into 44,132 contigs and 37,882 loci. Differentially expressed genes (DEGs) were identified and clustered according to their expression profile, with the majority being downregulated at 6 DAI, which coincides with the onset of the HR. Amongst these DEGs, 27 were selected for further qRT-PCR validation allowing the identifi- cation of nematode-responsive candidate genes that are putatively related to the resistance response. Those candidates are engaged in the salycilic (NBS-LRR, lipocalins, resveratrol synthase) and jasmonic (patatin, allene oxidase cyclase) acids pathways, and also related to hormonal balance (auxin responsive protein, GH3) and cellular plasticity and signaling (tetraspanin, integrin, expansin), with some of them showing contrasting expression behav-ior between Arachis RKN-resistant and susceptible genotypes. As these candidate genes activate different defensive signaling systems, the genetic (HR) and the induced resistance(IR), their pyramidding in one genotype via molecular breeding or transgenic strategy might contribute to a more durable resistance, thus improving the long-term control of RKN in peanut. 650 $aArachis Hypogaea 653 $aArachis spp 653 $aDiplóide selvagem 653 $aGene regulador 653 $aMelhoramento de planta 653 $aNematóide das galhas 653 $aPeanut 653 $aPeanut relatives 653 $aPlanta resistente 700 1 $aGUIMARAES, L. A. 700 1 $aMORGANTE, C. V. 700 1 $aSILVA JUNIOR, O. B. 700 1 $aARAUJO, A. C. G. 700 1 $aMARTINS, A. C. Q. 700 1 $aSARAIVA, M. A. P. 700 1 $aOLIVEIRA, T. N. 700 1 $aTOGAWA, R. C. 700 1 $aLEAL-BERTIOLI, S. C. M. 700 1 $aBERTIOLI, D. J. 700 1 $aBRASILEIRO, A. C. M. 773 $tPlos One, October 21, p. 1-22, 2015. (Open Access).
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
12/04/2012 |
Data da última atualização: |
18/11/2022 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
CECCHIN, D.; CAMPOS, A. T.; PIRES, M. de F. A.; ASSENHEIMER, A.; SOUSA, F. A.; PONCIANO, P. F.; YANAGI JUNIOR, T.; GONÇALVES JUNIOR, A. C. |
Afiliação: |
DAIANE CECCHIN, UFLA; ALESSANDRO TORRES CAMPOS, UFLA; MARIA DE FATIMA AVILA PIRES, CNPGL; ADRIANE ASSENHEIMER, Unioeste, Marechal C. Rondon, PR; FRANCINE APARECIDA SOUSA, UFLA; PATRÍCIA FERREIRA PONCIANO; TADAYUKI YANAGI JUNIOR, UFLA; AFFONSO CELSO GONÇALVES JUNIOR. |
Título: |
Avaliação físico-química de dejetos de bovinos tratados em tanques aerados. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
In: CONGRESSO INTERNACIONAL DO LEITE, 10.; WORKSHOP SOBRE POLÍTICAS PÚBLICAS, 10.; SIMPÓSIO DE SUSTENTABILIDADE DA ATIVIDADE LEITEIRA, 11., 2011, Maceió. Anais... Juiz de Fora: Embrapa Gado de Leite, 2011. |
Páginas: |
3 p. |
Descrição Física: |
1 CD-ROM. |
Idioma: |
Português |
Palavras-Chave: |
Aerador; Bovinocultura de leite; Lodo ativado por batelada; Resíduos. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/57504/1/Resumo34-XCongresso-Int.Leite.PDF
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Marc: |
LEADER 00890nam a2200241 a 4500 001 1922197 005 2022-11-18 008 2011 bl uuuu u00u1 u #d 100 1 $aCECCHIN, D. 245 $aAvaliação físico-química de dejetos de bovinos tratados em tanques aerados.$h[electronic resource] 260 $aIn: CONGRESSO INTERNACIONAL DO LEITE, 10.; WORKSHOP SOBRE POLÍTICAS PÚBLICAS, 10.; SIMPÓSIO DE SUSTENTABILIDADE DA ATIVIDADE LEITEIRA, 11., 2011, Maceió. Anais... Juiz de Fora: Embrapa Gado de Leite$c2011 300 $a3 p.$c1 CD-ROM. 653 $aAerador 653 $aBovinocultura de leite 653 $aLodo ativado por batelada 653 $aResíduos 700 1 $aCAMPOS, A. T. 700 1 $aPIRES, M. de F. A. 700 1 $aASSENHEIMER, A. 700 1 $aSOUSA, F. A. 700 1 $aPONCIANO, P. F. 700 1 $aYANAGI JUNIOR, T. 700 1 $aGONÇALVES JUNIOR, A. C.
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Embrapa Gado de Leite (CNPGL) |
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