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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
05/10/1993 |
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Data da última atualização: |
05/10/1993 |
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Autoria: |
GARCIA, M. L.; ARRESE, E. L.; GRAU, O.; SARACHU, A. N. |
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Afiliação: |
Catedra de Quimica Biologica II, Facultad de Ciencias Exactas, U. N.,La Plata 47 y 115, 1900 La Plata, Argentina. |
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Título: |
Citrus Psorosic Disease Agent Behaves as a two Componet ss RNA Virus. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Riverside, California: International Organization of Citrus Virologists, 1991. |
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Páginas: |
337-344 |
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Idioma: |
Inglês |
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Notas: |
Conference of the International Organization of Citrus Virologists, 11., 1991, Riverside, California, Proceedings... |
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Conteúdo: |
Citrus seedings were graft-inoculated with psorosis (isolate 90-1-1;Concordia, Argentina).When symptomatic young leaves were ground, homogeniized and centrifuged at low speed, infectivity (local lesions on Chenopodium quinoa) remained in the supernatant.Polyethylene glycol precipitation and high-speed centrifugation yielded infective pellets. Infectivity was abolished by RNase treatment, even in 0.3 M NaCI,indicating that ss RNA (s) is essential for biological activity. Protein(s) is also required for infectivity, since it was sensitivy to phenol extraction and proteinase K treatment. When high speed pellts were subjected to sucrose density gradient centrifugation, none of the single fractios accounted for loaded infectivity, but some of the two-fold combinations were much more highy infections than expected from the contribution of single fraction. Therefore two components activate each other for infectivity.Protein analysis of slow- and fast- sedimenting components sugeested that a protein of about 50 KD MW is associated with infectivity. We interprete these and other results as indicating that a bipartit virus is associated with psorosis disease. The virus has ss RNA as its genetic material and a 50 Kd polypeptide as one of its structural protein(s). |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01887naa a2200181 a 4500
001 1644269
005 1993-10-05
008 1991 bl uuuu u00u1 u #d
100 1 $aGARCIA, M. L.
245 $aCitrus Psorosic Disease Agent Behaves as a two Componet ss RNA Virus.
260 $c1991
300 $a337-344
500 $aConference of the International Organization of Citrus Virologists, 11., 1991, Riverside, California, Proceedings...
520 $aCitrus seedings were graft-inoculated with psorosis (isolate 90-1-1;Concordia, Argentina).When symptomatic young leaves were ground, homogeniized and centrifuged at low speed, infectivity (local lesions on Chenopodium quinoa) remained in the supernatant.Polyethylene glycol precipitation and high-speed centrifugation yielded infective pellets. Infectivity was abolished by RNase treatment, even in 0.3 M NaCI,indicating that ss RNA (s) is essential for biological activity. Protein(s) is also required for infectivity, since it was sensitivy to phenol extraction and proteinase K treatment. When high speed pellts were subjected to sucrose density gradient centrifugation, none of the single fractios accounted for loaded infectivity, but some of the two-fold combinations were much more highy infections than expected from the contribution of single fraction. Therefore two components activate each other for infectivity.Protein analysis of slow- and fast- sedimenting components sugeested that a protein of about 50 KD MW is associated with infectivity. We interprete these and other results as indicating that a bipartit virus is associated with psorosis disease. The virus has ss RNA as its genetic material and a 50 Kd polypeptide as one of its structural protein(s).
700 1 $aARRESE, E. L.
700 1 $aGRAU, O.
700 1 $aSARACHU, A. N.
773 $tRiverside, California: International Organization of Citrus Virologists, 1991.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
26/01/2016 |
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Data da última atualização: |
05/04/2016 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
PINTO, L. F. B.; MACHADO, A. L.; MEIRA, A. N.; COUTINHO, L. L.; MUNIZ, E. N.; AZEVEDO, H. C.; BITTENCOURT, T. C. B. dos S. C. de. |
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Afiliação: |
LUIS FERNANDO BATISTA PINTO; ALESSANDRO LIMA MACHADO; ARIANA NASCIEMTNO MEIRA; LUIZ LEHMANN COUTINHO; EVANDRO NEVES MUNIZ, CPATC; HYMERSON COSTA AZEVEDO, CPATC; THEREZA CRISTINA BORIO DOS SANTOS CALMON DE BITTENCOURT. |
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Título: |
Polimorfismos no gene CAST em ovinos Santa Inês. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE MELHORAMENTO ANIMAL, 11., 2015, Santa Maria, RS. Melhoramento animal da academia ao campo: uma parceria em construção: anais. Santa Maria, RS: SBMA: UFSM, 2015. |
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Idioma: |
Português |
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Palavras-Chave: |
Santa Inês. |
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Thesagro: |
Ovino. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/137853/1/sbma2015-39907-1438884469.pdf
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Marc: |
LEADER 00702nam a2200193 a 4500
001 2035281
005 2016-04-05
008 2015 bl uuuu u00u1 u #d
100 1 $aPINTO, L. F. B.
245 $aPolimorfismos no gene CAST em ovinos Santa Inês.$h[electronic resource]
260 $aIn: SIMPÓSIO BRASILEIRO DE MELHORAMENTO ANIMAL, 11., 2015, Santa Maria, RS. Melhoramento animal da academia ao campo: uma parceria em construção: anais. Santa Maria, RS: SBMA: UFSM$c2015
650 $aOvino
653 $aSanta Inês
700 1 $aMACHADO, A. L.
700 1 $aMEIRA, A. N.
700 1 $aCOUTINHO, L. L.
700 1 $aMUNIZ, E. N.
700 1 $aAZEVEDO, H. C.
700 1 $aBITTENCOURT, T. C. B. dos S. C. de
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Embrapa Tabuleiros Costeiros (CPATC) |
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