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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
11/02/2008 |
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Data da última atualização: |
12/02/2008 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BRASILEIRO-VIDAL, A. C.; SANTOS-SEREJO, J. A. dos; SOARES FILHO, W. dos S.; GUERRA, M. |
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Afiliação: |
A. C. Brasileiro-Vidal, UFPE; Janay Almeida dos Santos-Serejo, CNPMF; Walter dos Santos Soares Filho, CNPMF; M. Guerra, UFPE. |
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Título: |
A simple chromosomal marker can reliably distinguishes Poncirus from Citrus species. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Genetica, Netherlands, v. 129, n. 3, p. 273-279, mar. 2007. |
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Idioma: |
Português |
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Notas: |
DOI 10.1007/s10709-006-0007-4 |
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Conteúdo: |
Several chromosome types have been recognized in Citrus and related genera by chromomycin A3 (CMA) banding patterns and fluorescent in situ hybridization (FISH). They can be used to characterize cultivars and species or as markers in hybridization and backcrossing experiments. In the present work, characterization of six cultivars of P. trifoliata ("Barnes", "Fawcett", "Flying Dragon", "Pomeroy", "Rubidoux", "USDA") and one P. trifoliata × C. limonia hybrid was performed by sequential analyses of CMA banding and FISH using 5S and 45S rDNA as probes. All six cultivars showed a similar CMA+ banding pattern with the karyotype formula 4B + 8D + 6F. The capital letters indicate chromosomal types: B, a chromosome with one telomeric and one proximal band; D, with only one telomeric band; F, without bands. In situ hybridization labeling was also similar among cultivars. Three chromosome pairs displayed a closely linked set of 5S and 45S rDNA sites, two of them co-located with the proximal band of the B type chromosomes (B/5S-45S) and the third one co-located with the terminal band of a D pair (D/5S-45S). The B/5S-45S chromosome has never been found in any citrus accessions investigated so far. Therefore, this B chromosome can be used as a marker to recognize the intergeneric Poncirus × Citrus hybrids. The intergeneric hybrid analyzed here displayed the karyotype formula 4B + 8D + 6F, with two chromosome types B/5S-45S and two D/5S-45S. The karyotype formula and the presence of two B/5S-45S chromosomes clearly indicate that the plant investigated is a symmetric hybrid. It also demonstrates the suitability of karyotype analyses to differentiate zygotic embryos or somatic cell fusions involving trifoliate orange germplasm. MenosSeveral chromosome types have been recognized in Citrus and related genera by chromomycin A3 (CMA) banding patterns and fluorescent in situ hybridization (FISH). They can be used to characterize cultivars and species or as markers in hybridization and backcrossing experiments. In the present work, characterization of six cultivars of P. trifoliata ("Barnes", "Fawcett", "Flying Dragon", "Pomeroy", "Rubidoux", "USDA") and one P. trifoliata × C. limonia hybrid was performed by sequential analyses of CMA banding and FISH using 5S and 45S rDNA as probes. All six cultivars showed a similar CMA+ banding pattern with the karyotype formula 4B + 8D + 6F. The capital letters indicate chromosomal types: B, a chromosome with one telomeric and one proximal band; D, with only one telomeric band; F, without bands. In situ hybridization labeling was also similar among cultivars. Three chromosome pairs displayed a closely linked set of 5S and 45S rDNA sites, two of them co-located with the proximal band of the B type chromosomes (B/5S-45S) and the third one co-located with the terminal band of a D pair (D/5S-45S). The B/5S-45S chromosome has never been found in any citrus accessions investigated so far. Therefore, this B chromosome can be used as a marker to recognize the intergeneric Poncirus × Citrus hybrids. The intergeneric hybrid analyzed here displayed the karyotype formula 4B + 8D + 6F, with two chromosome types B/5S-45S and two D/5S-45S. The karyotype formula and the presence of two B... Mostrar Tudo |
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Palavras-Chave: |
Chromosome; CMA; rDNA. |
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Thesaurus Nal: |
Citrus; fish; Poncirus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02411naa a2200241 a 4500
001 1654619
005 2008-02-12
008 2007 bl --- 0-- u #d
100 1 $aBRASILEIRO-VIDAL, A. C.
245 $aA simple chromosomal marker can reliably distinguishes Poncirus from Citrus species.
260 $c2007
500 $aDOI 10.1007/s10709-006-0007-4
520 $aSeveral chromosome types have been recognized in Citrus and related genera by chromomycin A3 (CMA) banding patterns and fluorescent in situ hybridization (FISH). They can be used to characterize cultivars and species or as markers in hybridization and backcrossing experiments. In the present work, characterization of six cultivars of P. trifoliata ("Barnes", "Fawcett", "Flying Dragon", "Pomeroy", "Rubidoux", "USDA") and one P. trifoliata × C. limonia hybrid was performed by sequential analyses of CMA banding and FISH using 5S and 45S rDNA as probes. All six cultivars showed a similar CMA+ banding pattern with the karyotype formula 4B + 8D + 6F. The capital letters indicate chromosomal types: B, a chromosome with one telomeric and one proximal band; D, with only one telomeric band; F, without bands. In situ hybridization labeling was also similar among cultivars. Three chromosome pairs displayed a closely linked set of 5S and 45S rDNA sites, two of them co-located with the proximal band of the B type chromosomes (B/5S-45S) and the third one co-located with the terminal band of a D pair (D/5S-45S). The B/5S-45S chromosome has never been found in any citrus accessions investigated so far. Therefore, this B chromosome can be used as a marker to recognize the intergeneric Poncirus × Citrus hybrids. The intergeneric hybrid analyzed here displayed the karyotype formula 4B + 8D + 6F, with two chromosome types B/5S-45S and two D/5S-45S. The karyotype formula and the presence of two B/5S-45S chromosomes clearly indicate that the plant investigated is a symmetric hybrid. It also demonstrates the suitability of karyotype analyses to differentiate zygotic embryos or somatic cell fusions involving trifoliate orange germplasm.
650 $aCitrus
650 $afish
650 $aPoncirus
653 $aChromosome
653 $aCMA
653 $arDNA
700 1 $aSANTOS-SEREJO, J. A. dos
700 1 $aSOARES FILHO, W. dos S.
700 1 $aGUERRA, M.
773 $tGenetica, Netherlands$gv. 129, n. 3, p. 273-279, mar. 2007.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 120 | |
| 1. |  | SANTOS-SEREJO, J. A. dos. Como inovar em germoplasma de fruteiras. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. 1 CD-ROM (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 304).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 8. | | SILVA, S. de O. e; FERREIRA, C. F.; SANTOS-SEREJO, J. A. Banana. In: LOPES, M. A.; FÁVERO, A. P. FERREIRA, M. A. J. da; FALEIRO, F. G. (Org.). Curso internacional de pré-melhoramento de plantas. Brasília, DF : Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 165-166. (Embrapa Recursos Genético e Biotecnologia. Documentos, 185).| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 13. | | SANTOS-SEREJO, J. A. dos; BARBOSA, A. M. M.; LUNA, J. V. U. Tamarindo. In: SANTOS-SEREJO, J. A. dos; DANTAS, J. L. L.; SAMPAIO, C. V.; COELHO, Y. da S. (Ed.). Fruticultura tropical: espécies regionais e exóticas. Brasília, DF: Embrapa Informação Tecnológica, 2009. p. 437-455.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 19. | | SOUZA, F. V. D.; CABRAL, J. R. S.; SANTOS-SEREJO, J. A. dos; NEPOMUCENO, O. M. Híbridos de abacaxi com potencial ornamental. Revista Brasileira de Horticultura Ornamental, Campinas, v. 13, p.274-276, 2007. Suplemento. Edição dos Resumos do XVI Congresso Brasileiro de Floricultura e Plantas Ornamentais; III Congresso Brasileiro de Cultura de Tecidos e Plantas; I Simpósio de Plantas Ornamentais Nativas, Goiânia, set. 2007.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 120 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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