Data/hora: 12/06/2024 11:06:17

Biblioteca(s): 	Embrapa Milho e Sorgo.	
Data corrente: 	06/10/2021	
Data da última atualização: 	10/02/2022	
Autoria: 	STINGUEL, P.; PAIVA, C. A. C.; ZUIM, V.; AZEVEDO, A. C. T.; VALICENTE, F. H.; SANTOS JÚNIOR, H. J. G. dos.	
Afiliação: 	PRISCILA STINGUEL, Universidade Federal do Espírito Santo; CARLOS EDUARDO COSTA PAIVA, Universidade Federal do Espírito Santo; VITOR ZUIM; ANA CLARA THEZOLIN AZEVEDO, Universidade Federal do Espírito Santo; FERNANDO HERCOS VALICENTE, CNPMS; HUGO JOSÉ GONÇALVES DOS SANTOS JÚNIOR, Universidade Federal do Espírito Santo.	
Título: 	Optimization of in vivo production of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV).	
Ano de publicação: 	2022	
Fonte/Imprenta: 	Neotropical Entomology, v. 51, p. 122-132, 2022.	
DOI: 	https://doi.org/10.1007/s13744-021-00917-8	
Idioma: 	Inglês	
Notas: 	Publicado online em 29 set. 2021.	
Conteúdo: 	Insect viruses have been used to protect crops and forests worldwide for decades. Among insect viruses, isolates of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) have proven potential for the control of Spodoptera frugiperda (J. E. Smith) (FAW) (Lepidoptera: Noctuidae), a pest of many economically essential crops across several continents. Mass production of SfMNPV depends on an in vivo system using host insect rearing. However, many factors can limit its production, including abiotic factors and host characteristics, such as the stage of development and an antagonist intraspecifc interaction. Thus, to improve in vivo production, we verifed the most suitable larval age to inoculate the virus and the infuence of incubation temperature on viral production. Subsequently, cannibal behavior was verifed in FAW larvae reared at diferent densities, while reproducing the conditions of the best treatments. The highest viral yield occurred when FAW larvae were inoculated at 10 and 8 days old and incubated at 22 °C and 25 °C, respectively. Nonetheless, survival (lethal period in days) and cannibal behavior were positively infuenced by larval development, which potentially increases the load of contamination and requires larval individualization for these production conditions. In contrast, 4-day-old larvae, which were inoculated and incubated at 31 °C, also demonstrated high viral production, with lower rates of cannibalism and death on the same day, thereby showing potential. The information presented in this study is useful for the optimization of the in vivo production systems of SfMNPV.	
Palavras-Chave: 	Entomopathogenic; Fall armyworm; Lagarta do cartucho.	
Thesagro: 	Controle Biológico; Vírus.	
Thesaurus Nal: 	Baculoviridae; Biological control; Viruses.	
Categoria do assunto: 	S Ciências Biológicas	
Registro original: 	Embrapa Milho e Sorgo (CNPMS)	
Exemplares: 	CNPMS - (29621-1) - UPC - AP - DD -  -  -  - DIS