| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Ocidental. Para informações adicionais entre em contato com cpaa.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Amazônia Ocidental; Embrapa Meio Ambiente; Embrapa Pesca e Aquicultura. |
|
Data corrente: |
13/09/2012 |
|
Data da última atualização: |
11/03/2013 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SAMPAIO, F. G.; BOIJINK, C. de L.; SANTOS, L. R. B. dos; OBA, E. T.; KALININ, A. L.; LUIZ, A. J. B.; RANTIN, F. T. |
|
Afiliação: |
FERNANDA GARCIA SAMPAIO, CNPASA; CHEILA DE LIMA BOIJINK, CPAA; Laila Romagueira Bichara dos Santos, USP; ELIANE TIE OBA YOSHIOKA, CPAF-AP; Ana Lúcia Kalinin, FAPESP; ALFREDO JOSE BARRETO LUIZ, CNPMA; Francisco Tadeu Rantin, FAPESP. |
|
Título: |
Antioxidant defenses and biochemical changes in the neotropical fish pacu, Piaractus mesopotamicus: responses to single and combined copper and hypercarbia exposure. |
|
Ano de publicação: |
2012 |
|
Fonte/Imprenta: |
Comparative Biochemistry and Physiology, Part C, New York, v. 156, n. 3-4, p. 178-186, 2012. |
|
DOI: |
10.1016/j.cbpc.2012.07.002 |
|
Idioma: |
Inglês |
|
Conteúdo: |
This study investigated the potentially detrimental effects of copper and elevated aquatic CO2 (hypercarbia), alone or in combination, on pacu, Piaractus mesopotamicus. Fish were exposed for 48 h to control (no copper 2+ ?1addition in normocarbia), to 400 ?g Cu L , to hypercarbic (1% CO2; PCO2 = 6.9 mm Hg) water and to 400 ?g Cu2+L?1 +hypercarbia. In liver the single factors caused an increase in lipid hydroperoxide concen- tration that was not observed when the factors were combined. Copper exposure elicited increased hepatic superoxide dismutase activity, irrespective of aquatic CO2 level. On the other hand, the effects of copper on hepatic glutathione peroxidase activity were dependent on water CO2 levels. The two stressors combined did not affect hepatic catalase activity. Hypercarbic water caused a decline in plasma glucose concentration, but this was not observed when hypercarbia was combined with copper exposure. Copper caused a decrease in branchial Na+/K+-ATPase activity that was independent of water CO2 level. Copper caused an increase in branchial metallothionein concentration that was independent of water CO2 level. Thus, branchial metallothionein and Na+/K+ -ATPase were effective biomarkers of copper exposure that were not af- fected by water CO2 level. |
|
Palavras-Chave: |
Antioxidantes. |
|
Thesagro: |
Cobre; Dióxido de carbono; Pacu; Toxidez. |
|
Thesaurus Nal: |
Antioxidant activity; Carbon dioxide; Copper; Ecotoxicology; Fish; Resistance mechanisms. |
|
Categoria do assunto: |
--
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02342naa a2200337 a 4500
001 1952678
005 2013-03-11
008 2012 bl uuuu u00u1 u #d
024 7 $a10.1016/j.cbpc.2012.07.002$2DOI
100 1 $aSAMPAIO, F. G.
245 $aAntioxidant defenses and biochemical changes in the neotropical fish pacu, Piaractus mesopotamicus$bresponses to single and combined copper and hypercarbia exposure.$h[electronic resource]
260 $c2012
520 $aThis study investigated the potentially detrimental effects of copper and elevated aquatic CO2 (hypercarbia), alone or in combination, on pacu, Piaractus mesopotamicus. Fish were exposed for 48 h to control (no copper 2+ ?1addition in normocarbia), to 400 ?g Cu L , to hypercarbic (1% CO2; PCO2 = 6.9 mm Hg) water and to 400 ?g Cu2+L?1 +hypercarbia. In liver the single factors caused an increase in lipid hydroperoxide concen- tration that was not observed when the factors were combined. Copper exposure elicited increased hepatic superoxide dismutase activity, irrespective of aquatic CO2 level. On the other hand, the effects of copper on hepatic glutathione peroxidase activity were dependent on water CO2 levels. The two stressors combined did not affect hepatic catalase activity. Hypercarbic water caused a decline in plasma glucose concentration, but this was not observed when hypercarbia was combined with copper exposure. Copper caused a decrease in branchial Na+/K+-ATPase activity that was independent of water CO2 level. Copper caused an increase in branchial metallothionein concentration that was independent of water CO2 level. Thus, branchial metallothionein and Na+/K+ -ATPase were effective biomarkers of copper exposure that were not af- fected by water CO2 level.
650 $aAntioxidant activity
650 $aCarbon dioxide
650 $aCopper
650 $aEcotoxicology
650 $aFish
650 $aResistance mechanisms
650 $aCobre
650 $aDióxido de carbono
650 $aPacu
650 $aToxidez
653 $aAntioxidantes
700 1 $aBOIJINK, C. de L.
700 1 $aSANTOS, L. R. B. dos
700 1 $aOBA, E. T.
700 1 $aKALININ, A. L.
700 1 $aLUIZ, A. J. B.
700 1 $aRANTIN, F. T.
773 $tComparative Biochemistry and Physiology, Part C, New York$gv. 156, n. 3-4, p. 178-186, 2012.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pesca e Aquicultura (CNPASA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Agroenergia; Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
21/01/2010 |
|
Data da última atualização: |
04/02/2010 |
|
Tipo da produção científica: |
Orientação de Tese de Pós-Graduação |
|
Autoria: |
GARCIA, S. A. L. |
|
Afiliação: |
Suzana Antunes Lourenço Garcia, UFLA. |
|
Título: |
Identificação, validação e uso de marcadores moleculares a partir de sequências de DNA dos genomas de Mycosphaerella fijiensis e de Musa spp. |
|
Ano de publicação: |
2009 |
|
Fonte/Imprenta: |
2009. |
|
Páginas: |
74 f. |
|
Idioma: |
Inglês
Português |
|
Notas: |
Dissertação (Mestre em Biotecnologia Vegetal) - Universidade Federal de Lavras, Lavras. Manoel Teixeira Souza Júnior, Orientador. Cláudia Fortes Ferreira, CNPMF, Co-orientador. |
|
Conteúdo: |
Mycosphaerella fijiensis is the causal agent of black leaf streak of banana, the most important threat to banana production in many countries and particularly in Costa Rica where the climate is very conducive for the disease. Currently, the main control measure is the frequent application of fungicides. However, apart from environmental concerns, this approach is not sustainable due to the abrupt or gradual development of fungicide resistance. To analyze the population dynamics of fungicide resistance, we developed a molecular diagnostics for strobilurin resistance, using the cytochrome b gene (cytb), in M. fijiensis. We also developed molecular markers for the mating type idiomorphs (mat1-1 and mat1-2) and primers for five VNTR loci to estimate population genetic parameters. Monospore isolates were collected at three plantations that are 20-30 km apart (Cartagena, San Pablo and Zent) in the Limón province that represents the heart of the Costa Rican banana production area. Ninety-five isolates were obtained from a distant wild-type population that was never sprayed with fungicides in the Herédia province. In total, 665 isolates were assayed for mat1-1, mat1-2, VNTR and cytb. The mating type genes segregated in a 1:1 ratio indicating that the sampled populations most likely are randomly mating. The VNTR primers identified 32 alleles, among them, 21 were identified in wild type population, 20 in San Pablo population, 23 in Zent population and 24 in Cartegena population. The strobilurin diagnostic indicated that the wildtype population is entirely sensitive and that two of the three commercial populations, San Pablo and Cartagena are entirely resistant. The Zent population contained 8% of sensitive strains even though strobilurins were still used in that plantation. The next steps will be analysis of gene diversity within each population and the levels of gene flow. These analyses will provide an excellent basis for future research into fungicide resistance in Costa Rica populations of M. fijiensis as well as comparative analyses with other banana-producing areas. MenosMycosphaerella fijiensis is the causal agent of black leaf streak of banana, the most important threat to banana production in many countries and particularly in Costa Rica where the climate is very conducive for the disease. Currently, the main control measure is the frequent application of fungicides. However, apart from environmental concerns, this approach is not sustainable due to the abrupt or gradual development of fungicide resistance. To analyze the population dynamics of fungicide resistance, we developed a molecular diagnostics for strobilurin resistance, using the cytochrome b gene (cytb), in M. fijiensis. We also developed molecular markers for the mating type idiomorphs (mat1-1 and mat1-2) and primers for five VNTR loci to estimate population genetic parameters. Monospore isolates were collected at three plantations that are 20-30 km apart (Cartagena, San Pablo and Zent) in the Limón province that represents the heart of the Costa Rican banana production area. Ninety-five isolates were obtained from a distant wild-type population that was never sprayed with fungicides in the Herédia province. In total, 665 isolates were assayed for mat1-1, mat1-2, VNTR and cytb. The mating type genes segregated in a 1:1 ratio indicating that the sampled populations most likely are randomly mating. The VNTR primers identified 32 alleles, among them, 21 were identified in wild type population, 20 in San Pablo population, 23 in Zent population and 24 in Cartegena population. The s... Mostrar Tudo |
|
Palavras-Chave: |
Fungos; Transcriptoma; Trap; VNTR. |
|
Thesagro: |
Banana; DNA; Genoma; Marcador Molecular; Sigatoka Negra. |
|
Categoria do assunto: |
--
G Melhoramento Genético |
|
Marc: |
LEADER 02909nam a2200241 a 4500
001 1656314
005 2010-02-04
008 2009 bl uuuu m 00u1 u #d
100 1 $aGARCIA, S. A. L.
245 $aIdentificação, validação e uso de marcadores moleculares a partir de sequências de DNA dos genomas de Mycosphaerella fijiensis e de Musa spp.
260 $a2009.$c2009
300 $a74 f.
500 $aDissertação (Mestre em Biotecnologia Vegetal) - Universidade Federal de Lavras, Lavras. Manoel Teixeira Souza Júnior, Orientador. Cláudia Fortes Ferreira, CNPMF, Co-orientador.
520 $aMycosphaerella fijiensis is the causal agent of black leaf streak of banana, the most important threat to banana production in many countries and particularly in Costa Rica where the climate is very conducive for the disease. Currently, the main control measure is the frequent application of fungicides. However, apart from environmental concerns, this approach is not sustainable due to the abrupt or gradual development of fungicide resistance. To analyze the population dynamics of fungicide resistance, we developed a molecular diagnostics for strobilurin resistance, using the cytochrome b gene (cytb), in M. fijiensis. We also developed molecular markers for the mating type idiomorphs (mat1-1 and mat1-2) and primers for five VNTR loci to estimate population genetic parameters. Monospore isolates were collected at three plantations that are 20-30 km apart (Cartagena, San Pablo and Zent) in the Limón province that represents the heart of the Costa Rican banana production area. Ninety-five isolates were obtained from a distant wild-type population that was never sprayed with fungicides in the Herédia province. In total, 665 isolates were assayed for mat1-1, mat1-2, VNTR and cytb. The mating type genes segregated in a 1:1 ratio indicating that the sampled populations most likely are randomly mating. The VNTR primers identified 32 alleles, among them, 21 were identified in wild type population, 20 in San Pablo population, 23 in Zent population and 24 in Cartegena population. The strobilurin diagnostic indicated that the wildtype population is entirely sensitive and that two of the three commercial populations, San Pablo and Cartagena are entirely resistant. The Zent population contained 8% of sensitive strains even though strobilurins were still used in that plantation. The next steps will be analysis of gene diversity within each population and the levels of gene flow. These analyses will provide an excellent basis for future research into fungicide resistance in Costa Rica populations of M. fijiensis as well as comparative analyses with other banana-producing areas.
650 $aBanana
650 $aDNA
650 $aGenoma
650 $aMarcador Molecular
650 $aSigatoka Negra
653 $aFungos
653 $aTranscriptoma
653 $aTrap
653 $aVNTR
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
