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Registro Completo |
Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
30/03/2016 |
Data da última atualização: |
24/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SANTOS, L. F. dos; FREGAPANI, R. M.; FALCAO, L. L.; TOGAWA, R. C.; COSTA, M. M. do C.; LOPES, U. V.; GRAMACHO, K. P.; ALVES, R. M.; MICHELI, F.; MARCELLINO, L. H. |
Afiliação: |
Lucas Ferraz dos Santos, UESC/CENARGEN; Roberta Moreira Fregapani, CENARGEN; LOENI LUDKE FALCAO, CENARGEN; ROBERTO COITI TOGAWA, CENARGEN; MARCOS MOTA DO CARMO COSTA, CENARGEN; Uilson Vanderlei Lopes, CEPLAC/CEPEC; Karina Peres Gramacho, CEPLAC/CEPEC; RAFAEL MOYSES ALVES, CPATU; Fabienne Micheli, UESC/CIRAD; LUCILIA HELENA MARCELLINO, CENARGEN. |
Título: |
First microsatellite markers developed from cupuassu ESTs: application in diversity analysis and cross-species transferability to cacao. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
PLoS ONE, v. 11, n. 3, e0151074, 2016. |
DOI: |
10.1371/journal.pone.0151074 |
Idioma: |
Inglês |
Conteúdo: |
The cupuassu tree (Theobroma grandiflorum) (Willd. ex Spreng.) Schum. is a fruitful species from the Amazon with great economical potential, due to the multiple uses of its fruit´s pulp and seeds in the food and cosmetic industries, including the production of cupulate, an alternative to chocolate. In order to support the cupuassu breeding program and to select plants presenting both pulp/seed quality and fungal disease resistance, SSRs from Next Generation Sequencing ESTs were obtained and used in diversity analysis. From 8,330 ESTs, 1,517 contained one or more SSRs (1,899 SSRs identified). The most abundant motifs identified in the EST-SSRs were hepta- and trinucleotides, and they were found with a minimum and maximum of 2 and 19 repeats, respectively. From the 1,517 ESTs containing SSRs, 70 ESTs were selected based on their functional annotation, focusing on pulp and seed quality, as well as resistance to pathogens. The 70 ESTs selected contained 77 SSRs, and among which, 11 were polymorphic in cupuassu genotypes. These EST-SSRs were able to discriminate the cupuassu genotype in relation to resistance/susceptibility to witches? broom disease, as well as to pulp quality (SST/ATT values). Finally, we showed that these markers were transferable to cacao genotypes, and that genome availability might be used as a predictive tool for polymorphism detection and primer design useful for both Theobroma species. To our knowledge, this is the first report involving EST-SSRs from cupuassu and is also a pioneer in the analysis of marker transferability from cupuassu to cacao. Moreover, these markers might contribute to develop or saturate the cupuassu and cacao genetic maps, respectively. MenosThe cupuassu tree (Theobroma grandiflorum) (Willd. ex Spreng.) Schum. is a fruitful species from the Amazon with great economical potential, due to the multiple uses of its fruit´s pulp and seeds in the food and cosmetic industries, including the production of cupulate, an alternative to chocolate. In order to support the cupuassu breeding program and to select plants presenting both pulp/seed quality and fungal disease resistance, SSRs from Next Generation Sequencing ESTs were obtained and used in diversity analysis. From 8,330 ESTs, 1,517 contained one or more SSRs (1,899 SSRs identified). The most abundant motifs identified in the EST-SSRs were hepta- and trinucleotides, and they were found with a minimum and maximum of 2 and 19 repeats, respectively. From the 1,517 ESTs containing SSRs, 70 ESTs were selected based on their functional annotation, focusing on pulp and seed quality, as well as resistance to pathogens. The 70 ESTs selected contained 77 SSRs, and among which, 11 were polymorphic in cupuassu genotypes. These EST-SSRs were able to discriminate the cupuassu genotype in relation to resistance/susceptibility to witches? broom disease, as well as to pulp quality (SST/ATT values). Finally, we showed that these markers were transferable to cacao genotypes, and that genome availability might be used as a predictive tool for polymorphism detection and primer design useful for both Theobroma species. To our knowledge, this is the first report involving EST-SSRs from cup... Mostrar Tudo |
Palavras-Chave: |
Melhoramento genético. |
Thesagro: |
Cacau; Cupuaçu; Fruta Tropical. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141829/1/2016Santos.pdf
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Marc: |
LEADER 02580naa a2200289 a 4500 001 2052633 005 2023-03-24 008 2016 bl uuuu u00u1 u #d 024 7 $a10.1371/journal.pone.0151074$2DOI 100 1 $aSANTOS, L. F. dos 245 $aFirst microsatellite markers developed from cupuassu ESTs$bapplication in diversity analysis and cross-species transferability to cacao.$h[electronic resource] 260 $c2016 520 $aThe cupuassu tree (Theobroma grandiflorum) (Willd. ex Spreng.) Schum. is a fruitful species from the Amazon with great economical potential, due to the multiple uses of its fruit´s pulp and seeds in the food and cosmetic industries, including the production of cupulate, an alternative to chocolate. In order to support the cupuassu breeding program and to select plants presenting both pulp/seed quality and fungal disease resistance, SSRs from Next Generation Sequencing ESTs were obtained and used in diversity analysis. From 8,330 ESTs, 1,517 contained one or more SSRs (1,899 SSRs identified). The most abundant motifs identified in the EST-SSRs were hepta- and trinucleotides, and they were found with a minimum and maximum of 2 and 19 repeats, respectively. From the 1,517 ESTs containing SSRs, 70 ESTs were selected based on their functional annotation, focusing on pulp and seed quality, as well as resistance to pathogens. The 70 ESTs selected contained 77 SSRs, and among which, 11 were polymorphic in cupuassu genotypes. These EST-SSRs were able to discriminate the cupuassu genotype in relation to resistance/susceptibility to witches? broom disease, as well as to pulp quality (SST/ATT values). Finally, we showed that these markers were transferable to cacao genotypes, and that genome availability might be used as a predictive tool for polymorphism detection and primer design useful for both Theobroma species. To our knowledge, this is the first report involving EST-SSRs from cupuassu and is also a pioneer in the analysis of marker transferability from cupuassu to cacao. Moreover, these markers might contribute to develop or saturate the cupuassu and cacao genetic maps, respectively. 650 $aCacau 650 $aCupuaçu 650 $aFruta Tropical 653 $aMelhoramento genético 700 1 $aFREGAPANI, R. M. 700 1 $aFALCAO, L. L. 700 1 $aTOGAWA, R. C. 700 1 $aCOSTA, M. M. do C. 700 1 $aLOPES, U. V. 700 1 $aGRAMACHO, K. P. 700 1 $aALVES, R. M. 700 1 $aMICHELI, F. 700 1 $aMARCELLINO, L. H. 773 $tPLoS ONE$gv. 11, n. 3, e0151074, 2016.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
Data corrente: |
13/09/2022 |
Data da última atualização: |
04/11/2022 |
Tipo da produção científica: |
Documentos |
Autoria: |
PINHEIRO, J. O. C.; BERNI, R. F.; CYSNE, A. Q. |
Afiliação: |
JOSE OLENILSON COSTA PINHEIRO, CPAA; RODRIGO FASCIN BERNI, CPAA; ALEX QUEIROZ CYSNE, CNPAT. |
Título: |
Análise econômica e de custos para sistema de cultivo de tomateiro do tipo italiano, sob cultivo protegido e em substrato, na região de Manaus, AM. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Manaus: Embrapa Amazônia Ocidental, 2022. |
Páginas: |
24 p. |
Série: |
(Embrapa Amazônia Ocidental. Documentos, 159). |
ISSN: |
1517-3135 |
Idioma: |
Português |
Notas: |
ODS 2 |
Conteúdo: |
Este estudo teve como objetivo analisar os custos de implantação, manejo e produção de um cultivo protegido de tomate de mesa para a cidade de Manaus, avaliando a rentabilidade econômica na condução dessa atividade. |
Palavras-Chave: |
Combate a Fome; Lycopersicum esculentum; Objetivo de desenvolvimento sustentável; ODS 2. |
Thesagro: |
Análise Econômica; Cultivo Protegido; Tomate. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1146384/1/Doc159.pdf
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Marc: |
LEADER 01079nam a2200265 a 4500 001 2146384 005 2022-11-04 008 2022 bl uuuu u0uu1 u #d 022 $a1517-3135 100 1 $aPINHEIRO, J. O. C. 245 $aAnálise econômica e de custos para sistema de cultivo de tomateiro do tipo italiano, sob cultivo protegido e em substrato, na região de Manaus, AM.$h[electronic resource] 260 $aManaus: Embrapa Amazônia Ocidental$c2022 300 $a24 p. 490 $a(Embrapa Amazônia Ocidental. Documentos, 159). 500 $aODS 2 520 $aEste estudo teve como objetivo analisar os custos de implantação, manejo e produção de um cultivo protegido de tomate de mesa para a cidade de Manaus, avaliando a rentabilidade econômica na condução dessa atividade. 650 $aAnálise Econômica 650 $aCultivo Protegido 650 $aTomate 653 $aCombate a Fome 653 $aLycopersicum esculentum 653 $aObjetivo de desenvolvimento sustentável 653 $aODS 2 700 1 $aBERNI, R. F. 700 1 $aCYSNE, A. Q.
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