Data/hora: 16/06/2024 02:58:57

Biblioteca(s): 	Embrapa Pecuária Sul.	
Data corrente: 	27/11/2020	
Data da última atualização: 	27/11/2020	
Autoria: 	COMIN, H. B.; DOMINGUES, R.; GASPAR, E. B.; SANTOS, J. R. G. de los; CARDOSO, F. F.	
Afiliação: 	Helena Brocardo Comin, UFPEL; ROBERT DOMINGUES, CPPSUL; EMANUELLE BALDO GASPAR, CPPSUL; João Rodrigo Gil De Los Santos, UFPEL; FERNANDO FLORES CARDOSO, CPPSUL.	
Título: 	Genetic differences among Moraxella bovis and Moraxella bovoculi isolates from infectious bovine keratoconjunctivitis (IBK) outbreaks in southern Brazil.	
Ano de publicação: 	2020	
Fonte/Imprenta: 	Genetics and Molecular Biology, v. 43, n. 2, e20180380, p. 1-7, 2020.	
Idioma: 	Inglês	
Conteúdo: 	The objective of this study was to evaluate the genetic diversity ofMoraxella bovisandMoraxella bovoculibacteriaisolated from infectious bovine keratoconjunctivitis (IBK) outbreaks in the state of Rio Grande do Sul, Brazil. The ge-netic diversity amongMoraxellaspp. was evaluated by RAPD-PCR, JWP1-JWOPA07-PCR, ERIC-PCR and by se-quencing the 16S-23S intergenic regions. Based on the dendrogram, two genetically differentiated clades wereobserved; 14 isolates were classified asM. bovisand 17 asM. bovoculi. Genetic distances between theM. bovissamples ranged from 0.0379 to 0.4285, while forM. bovoculithe dissimilarities ranged from zero to 0.7297. Alterna-tively, based on sequencing analyses of the 16S-23S intergenic region,M. bovisandM. bovoculiisolates weregrouped into the same two different clades, but it was not possible to differentiate between isolates within clades.PCR techniques were demonstrated to be a satisfactory tool to unravel the genetic variability amongMoraxellaspp.,while sequencing of the 16S-23S intergenic region was only able to differentiate two species of theMoraxellagenus.Despite sampling geographically close regions, we demonstrate considerable genetic diversity inM. bovisandM.bovoculistrains and genetically distinctM. bovisstrains co-infecting the same animal.	
Thesagro: 	Bovino; Doença Animal; Genética Animal; Olho.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Pecuária Sul (CPPSUL)	
Exemplares: 	CPPSUL - (14711-1) - UPC - AP - DD -  -  -  - DIS