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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
25/01/2016 |
Data da última atualização: |
03/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ROSSONI, R. D.; BARBOSA, J. O.; VILELA, S. F. G.; SANTOS, J. D. dos; BARROS, P. P. de; PRATA, M. C. de A.; ANBINDER, A. L.; FUCHS, B. B.; JORGE, A. O. C.; MYLONAKIS, E.; JUNQUEIRA, J. C. |
Afiliação: |
Rodnei Dennis Rossoni; Júnia Oliveira Barbosa; Simone Furgeri Godinho Vilela; Jéssica Diane dos Santos; Patrícia Pimentel de Barros; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; Ana Lia Anbinder; Beth Burgwyn Fuchs; Antonio Olavo Cardoso Jorge; Eleftherios Mylonakis; Juliana Campos Junqueira. |
Título: |
Competitive Interactions between C. albicans, C. glabrata and C. krusei during Biofilm Formation and Development of Experimental Candidiasis. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
PLoS One, v. 10, n. 7, 2015. |
Páginas: |
23 p. |
Idioma: |
Português |
Conteúdo: |
In this study, we evaluated the interactions between Candida albicans, Candida krusei and Candida glabrata in mixed infections. Initially, these interactions were studied in biofilms formed in vitro. CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively. After that, we expanded this study for in vivo host models of experimental candidiasis. G. mellonella larvae were inoculated with monotypic and heterotypic Candida suspensions for analysis of survival rate and quantification of fungal cells in the haemolymph. In the groups with single infections, 100% of the larvae died within 18 h after infection with C. albicans. However, interaction groups achieved 100% mortality after 72 h of infection by C. albicans-C. glabrata and 96 h of infection by C. albicans-C. krusei. C. albicans CFU/mL values from larvae hemolymph were lower in the interacting groups compared with the monoespecies group after 12 h of infection. In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis. C. albicans CFU/mL values recovered from oral cavity of mice were higher in the group with single infection by C. albicans than the groups with mixed infections by C. albicans-C. glabrata and C. albicans-C. krusei. Moreover, the group with single infection by C. albicans had a higher degree of hyphae and epithelial changes in the tongue dorsum than the groups with mixed infections. We concluded that single infections by C. albicans were more harmful for animal models than mixed infections with non-albicans species, suggesting that C. albicans establish competitive interactions with C. krusei and C. glabrata during biofilm formation and development of experimental candidiasis. MenosIn this study, we evaluated the interactions between Candida albicans, Candida krusei and Candida glabrata in mixed infections. Initially, these interactions were studied in biofilms formed in vitro. CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively. After that, we expanded this study for in vivo host models of experimental candidiasis. G. mellonella larvae were inoculated with monotypic and heterotypic Candida suspensions for analysis of survival rate and quantification of fungal cells in the haemolymph. In the groups with single infections, 100% of the larvae died within 18 h after infection with C. albicans. However, interaction groups achieved 100% mortality after 72 h of infection by C. albicans-C. glabrata and 96 h of infection by C. albicans-C. krusei. C. albicans CFU/mL values from larvae hemolymph were lower in the interacting groups compared with the monoespecies group after 12 h of infection. In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis. C. albicans CFU/mL values recovered from oral cavity of mice were higher in the group with single infection by C. albicans than the groups with mixed infections by C. albicans-C. glabrata and C. albicans-C. krusei. Moreover, the group with single infection by C. albicans ha... Mostrar Tudo |
Palavras-Chave: |
Animal model. |
Thesaurus Nal: |
Galleria mellonella; infection. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/137696/1/Cnpgl-2015-PlosOne-Competitive.pdf
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Marc: |
LEADER 02761naa a2200289 a 4500 001 2035034 005 2024-02-03 008 2015 bl uuuu u00u1 u #d 100 1 $aROSSONI, R. D. 245 $aCompetitive Interactions between C. albicans, C. glabrata and C. krusei during Biofilm Formation and Development of Experimental Candidiasis.$h[electronic resource] 260 $c2015 300 $a23 p. 520 $aIn this study, we evaluated the interactions between Candida albicans, Candida krusei and Candida glabrata in mixed infections. Initially, these interactions were studied in biofilms formed in vitro. CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively. After that, we expanded this study for in vivo host models of experimental candidiasis. G. mellonella larvae were inoculated with monotypic and heterotypic Candida suspensions for analysis of survival rate and quantification of fungal cells in the haemolymph. In the groups with single infections, 100% of the larvae died within 18 h after infection with C. albicans. However, interaction groups achieved 100% mortality after 72 h of infection by C. albicans-C. glabrata and 96 h of infection by C. albicans-C. krusei. C. albicans CFU/mL values from larvae hemolymph were lower in the interacting groups compared with the monoespecies group after 12 h of infection. In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis. C. albicans CFU/mL values recovered from oral cavity of mice were higher in the group with single infection by C. albicans than the groups with mixed infections by C. albicans-C. glabrata and C. albicans-C. krusei. Moreover, the group with single infection by C. albicans had a higher degree of hyphae and epithelial changes in the tongue dorsum than the groups with mixed infections. We concluded that single infections by C. albicans were more harmful for animal models than mixed infections with non-albicans species, suggesting that C. albicans establish competitive interactions with C. krusei and C. glabrata during biofilm formation and development of experimental candidiasis. 650 $aGalleria mellonella 650 $ainfection 653 $aAnimal model 700 1 $aBARBOSA, J. O. 700 1 $aVILELA, S. F. G. 700 1 $aSANTOS, J. D. dos 700 1 $aBARROS, P. P. de 700 1 $aPRATA, M. C. de A. 700 1 $aANBINDER, A. L. 700 1 $aFUCHS, B. B. 700 1 $aJORGE, A. O. C. 700 1 $aMYLONAKIS, E. 700 1 $aJUNQUEIRA, J. C. 773 $tPLoS One$gv. 10, n. 7, 2015.
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Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
18/04/2008 |
Data da última atualização: |
20/10/2009 |
Tipo da produção científica: |
Documentos |
Autoria: |
REIS JÚNIOR, F. B. dos; MENDES, I. de C. |
Afiliação: |
Fábio Bueno dos Reis Júnior, CPAC; Iêda de Carvalho Mendes, CPAC. |
Título: |
Biomassa microbiana do solo. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
Planaltina, DF: Embrapa Cerrados, 2007. |
Páginas: |
40 p. |
Série: |
(Embrapa Cerrados. Documentos, 205). |
Idioma: |
Português |
Conteúdo: |
ABSTRACT: The microbial biomass, respiration and their derivative indexes are proposed as indicators of the state and changes of the soil organic matter. This document presents basic information about these indicators and the two methodologies most used for C-microbial biomass determinations, the fumigation-incubation and the fumigation-extraction methods. The basic principles behind these methods, advantages, disadvantages and limitations are discussed. As then main objective of this review, recent results from research in this field developed at the Embrapa Cerrados are presented. |
Palavras-Chave: |
Incubation; Microbial flora. |
Thesagro: |
Biomassa; Carbono; Cerrado; Fumigação; Incubação; Matéria Orgânica; Microbiologia; Microbiologia do Solo; População Microbiana; Solo. |
Thesaurus NAL: |
biomass; fumigation; microbiology; soil. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPAC-2009/28922/1/doc_205.pdf
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Marc: |
LEADER 01428nam a2200337 a 4500 001 1572256 005 2009-10-20 008 2007 bl uuuu u0uu1 u #d 100 1 $aREIS JÚNIOR, F. B. dos 245 $aBiomassa microbiana do solo. 260 $aPlanaltina, DF: Embrapa Cerrados$c2007 300 $a40 p. 490 $a(Embrapa Cerrados. Documentos, 205). 520 $aABSTRACT: The microbial biomass, respiration and their derivative indexes are proposed as indicators of the state and changes of the soil organic matter. This document presents basic information about these indicators and the two methodologies most used for C-microbial biomass determinations, the fumigation-incubation and the fumigation-extraction methods. The basic principles behind these methods, advantages, disadvantages and limitations are discussed. As then main objective of this review, recent results from research in this field developed at the Embrapa Cerrados are presented. 650 $abiomass 650 $afumigation 650 $amicrobiology 650 $asoil 650 $aBiomassa 650 $aCarbono 650 $aCerrado 650 $aFumigação 650 $aIncubação 650 $aMatéria Orgânica 650 $aMicrobiologia 650 $aMicrobiologia do Solo 650 $aPopulação Microbiana 650 $aSolo 653 $aIncubation 653 $aMicrobial flora 700 1 $aMENDES, I. de C.
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