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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
17/02/2009 |
Data da última atualização: |
07/03/2017 |
Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
Autoria: |
ABREU, A. de F. B.; DEL PELOSO, M. J.; RAMALHO, M. A. P.; CARNEIRO, J. E. de S.; PAULA JÚNIOR, T. J. de; PEREIRA FILHO, I. A.; MARTINS, M.; FARIA, L. C. de; MELO, L. C.; COSTA, J. G. C. da; RAVA, C. A.; SARTORATO, A.; FARIA, J. C. de; SILVA H. T. da; BARROS, E. G. de; SANTOS, J. B. dos; MOREIRA, M. A. |
Afiliação: |
ANGELA DE FATIMA BARBOSA ABREU, CNPAF; MARIA JOSE DEL PELOSO, CNPAF; Magno Antonio Patto Ramalho, UFLA; José Eustáquio de Souza Carneiro, UFV; Trazilbo José de Paula Júnior, EPAMIG; ISRAEL ALEXANDRE PEREIRA FILHO, CNPMS; Maurício Martins, UFU; LUIS CLAUDIO DE FARIA, CNPAF; LEONARDO CUNHA MELO, CNPAF; JOAQUIM GERALDO CAPRIO DA COSTA, CNPAF; CARLOS AGUSTIN RAVA, CNPAF; ALOISIO SARTORATO, CNPAF; JOSIAS CORREA DE FARIA, CNPAF; HELOISA TORRES DA SILVA, CNPAF; Everaldo Gonçalves de Barros, UFV; João Bosco dos Santos, UFLA; Maurílio Alves Moreira, UFV. |
Título: |
BRS 7762 Supremo: cultivar de feijão comum de grãos preto e porte ereto para Minas Gerais. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2008. |
Páginas: |
2 p. |
Série: |
(Embrapa Arroz e Feijão. Comunicado técnico, 160). |
Idioma: |
Português |
Conteúdo: |
A cultivar BRS 7762 Supremo é resultante do cruzamento biparental entre as linhagens W22-34 e VAN163, realizado na Embrapa Arroz e Feijão, em 1988. Apresenta hábito de crescimento indeterminado, com porte da planta ereto e boa resistência ao acamamento, em qualquer sistema de plantio. A arquitetura da planta possibilita a colheita mecânica; também é interessante os produtores que plantam feijão intercalado nos cafezais, prática muito comum no Estado de Minas Gerais, uma vez que a ausência de "guias" longas, facilita o manejo da cultura do feijoeiro e do café, ao qual está associada. |
Palavras-Chave: |
BRS 7762 Supremo; Grão preto. |
Thesagro: |
Feijão; Melhoramento Genético Vegetal; Phaseolus Vulgaris. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPAF-2009-09/27960/1/comt_160.pdf
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Marc: |
LEADER 01761nam a2200385 a 4500 001 1216264 005 2017-03-07 008 2008 bl uuuu u0uu1 u #d 100 1 $aABREU, A. de F. B. 245 $aBRS 7762 Supremo$bcultivar de feijão comum de grãos preto e porte ereto para Minas Gerais.$h[electronic resource] 260 $aSanto Antônio de Goiás: Embrapa Arroz e Feijão$c2008 300 $a2 p. 490 $a(Embrapa Arroz e Feijão. Comunicado técnico, 160). 520 $aA cultivar BRS 7762 Supremo é resultante do cruzamento biparental entre as linhagens W22-34 e VAN163, realizado na Embrapa Arroz e Feijão, em 1988. Apresenta hábito de crescimento indeterminado, com porte da planta ereto e boa resistência ao acamamento, em qualquer sistema de plantio. A arquitetura da planta possibilita a colheita mecânica; também é interessante os produtores que plantam feijão intercalado nos cafezais, prática muito comum no Estado de Minas Gerais, uma vez que a ausência de "guias" longas, facilita o manejo da cultura do feijoeiro e do café, ao qual está associada. 650 $aFeijão 650 $aMelhoramento Genético Vegetal 650 $aPhaseolus Vulgaris 653 $aBRS 7762 Supremo 653 $aGrão preto 700 1 $aDEL PELOSO, M. J. 700 1 $aRAMALHO, M. A. P. 700 1 $aCARNEIRO, J. E. de S. 700 1 $aPAULA JÚNIOR, T. J. de 700 1 $aPEREIRA FILHO, I. A. 700 1 $aMARTINS, M. 700 1 $aFARIA, L. C. de 700 1 $aMELO, L. C. 700 1 $aCOSTA, J. G. C. da 700 1 $aRAVA, C. A. 700 1 $aSARTORATO, A. 700 1 $aFARIA, J. C. de 700 1 $aSILVA H. T. da 700 1 $aBARROS, E. G. de 700 1 $aSANTOS, J. B. dos 700 1 $aMOREIRA, M. A.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Tabuleiros Costeiros. Para informações adicionais entre em contato com cpatc.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
19/12/2023 |
Data da última atualização: |
29/12/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
C - 0 |
Autoria: |
SANTOS, K. S.; COSTA, C.; BESSA, M. J.; TEIXEIRA, J. P.; SILVA, A. V. C. da; PADILHA, F. F.; DARIVA, C.; OLIVEIRA, M. B. P. P. |
Afiliação: |
KLEBSON SILVA SANTOS, ITP; CARLA COSTA, Portuguese National Institute of Health; MARIA JOÃO BESSA, Universidade do Porto; JOAO PAULO TEIXEIRA, UNIVERSIDADE DO PORTO; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC; FRANCINE FERREIRA PADILHA, ITP; CLÁUDIO DARIVA, ITP; MARIA BEATRIZ PINTO PRIOR OLIVEIRA, UNIVERSIDADE DO PORTO. |
Título: |
Azadirachta indica A. Juss (neem) phenolic extract inhibits human B-lymphoblastoid cells growth via cell cycle arrest, apoptosis induction, and DNA damage. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Exploration of Foods and Foodomics, v. 1, p. 130-142, 2023. |
DOI: |
https://doi.org/10.37349/eff.2023.00011 |
Idioma: |
Inglês |
Conteúdo: |
Aim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed
from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negative control (NC) to 83% after treatment with neem extracts (15 μg/mL). Neem extracts were also able to cause DNA strand breaks in TK6 cells. MenosAim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed
from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negati... Mostrar Tudo |
Thesagro: |
Conservação; Nim; Planta Medicinal. |
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
Marc: |
LEADER 02468naa a2200253 a 4500 001 2160010 005 2023-12-29 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.37349/eff.2023.00011$2DOI 100 1 $aSANTOS, K. S. 245 $aAzadirachta indica A. Juss (neem) phenolic extract inhibits human B-lymphoblastoid cells growth via cell cycle arrest, apoptosis induction, and DNA damage.$h[electronic resource] 260 $c2023 520 $aAim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negative control (NC) to 83% after treatment with neem extracts (15 μg/mL). Neem extracts were also able to cause DNA strand breaks in TK6 cells. 650 $aConservação 650 $aNim 650 $aPlanta Medicinal 700 1 $aCOSTA, C. 700 1 $aBESSA, M. J. 700 1 $aTEIXEIRA, J. P. 700 1 $aSILVA, A. V. C. da 700 1 $aPADILHA, F. F. 700 1 $aDARIVA, C. 700 1 $aOLIVEIRA, M. B. P. P. 773 $tExploration of Foods and Foodomics$gv. 1, p. 130-142, 2023.
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