Data/hora: 04/06/2024 07:58:29

Biblioteca(s): | Embrapa Pecuária Sudeste.
Data corrente: | 30/11/2023
Data da última atualização: | 30/11/2023
Autoria: | SANTOS, H. M.; NICIURA, S. C. M.; NOGUEIRA, A. R. de A.
Afiliação: | HERICK MACEDO SANTOS, Federal University of São Carlos; SIMONE CRISTINA MEO NICIURA, CPPSE; ANA RITA DE ARAUJO NOGUEIRA, CPPSE.
Título: | Analytical strategies and method validation to access the bioavailability of essential elements and arsenic from raw and cooked fish and shrimp.
Ano de publicação: | 2023
Fonte/Imprenta: | Food Science & Technology, v. 3, n. 7, 2023.
Páginas: | p. 1229-1237.
DOI: | 10.1021/acsfoodscitech.3c00132
Idioma: | Inglês
Conteúdo: | The bioavailability of essential and toxic elements in raw/cooked tilapia and shrimp was estimated using in vitro gastrointestinal digestion followed by the Caco-2 cell model to simulate the human intestinal barrier. Analytical strategies such as aerosol dilution, collision cell, and matrix-matching calibration were successfully explored for multielement quantification using ICP OES and ICP-MS. From Caco-2 cell bioassays, the estimated bioavailability values ranged from 1.4% (Fe) to 15.2% (Cu) in tilapia and from 0.2% (Fe) to 10.7% (P) in shrimp. Air-frying and oven-baking methods decreased tilapia’s Mg, P, S, Cu, and Fe bioavailability. Contrarily, the bioavailability of essential elements (except Mn) in shrimp seemed to increase after air-frying. Arsenic bioavailability in cooked shrimp was significantly higher than in the raw sample (1.9 vs 5.7%) but fortunately below the limit allowed by legislation. In spite of the complexity of basal solutions from the cell-based bioassays, the applied analytical strategies allowed the accurate quantification of the bioavailable fractions by ICP-based methods.
Palavras-Chave: | Analytical strategies; Caco-2 cells; In vitro uptake; Thermal treatments.
Categoria do assunto: | W Química e Física
Registro original: | Embrapa Pecuária Sudeste (CPPSE)
Exemplares: | CPPSE - (26250-1) - UPC - AP - DD - PROCI-2023.00108 - SAN - 2023.00281 - DIS