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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
13/11/2019 |
Data da última atualização: |
13/11/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
TOMASI, J. de C.; DEGENHARDT-GOLDBACH, J.; GRUNENNVALDT, R. L.; SANTOS, G. D. dos; VICENTE, V. A.; FRANCISCON, L.; BONA, C.; QUOIRIN, M. |
Afiliação: |
Jéssica de Cássia Tomasi, UFPR; JULIANA DEGENHARDT GOLDBACH, CNPF; Renata Lucia Grunennvaldt, UFPR; Germana Dávila dos Santos, UFPR; Vania Aparecida Vicente, UFPR; LUZIANE FRANCISCON, CNPF; Cleusa Bona, UFPR; Marguerite Quoirin, UFPR. |
Título: |
In vitro establishment of shoot meristems of Ilex paraguariensis and identification of endophytic bacteria. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Journal of Forestry Research, v. 30, n. 5, p. 1765-1777, Oct. 2019. |
DOI: |
10.1007/s11676-018-0763-x |
Idioma: |
Inglês |
Conteúdo: |
Yerba mate (Ilex paraguariensis A. St.-Hil.) is a species of great economic, social and environmental importance for the southern regions of Brazil, Uruguay and Argentina. Currently the most diverse products are obtained from mate leaves, including mate tea. The objective of this study was to establish shoot meristem cultures (meristematic dome and a few primordia) of elite clones and identify the endophytic bacteria present in the explants. We tested the effect of clones (F1, F2, A03 and A07), culture media (MS, 1/2MS, 1/4MS and WPM), cytokinins (kinetin, BA and 2iP), activated charcoal (1, 2 and 3 g L-1), and disinfecting agent (sodium hypochlorite and mercuric chloride) on in vitro establishment. F1 and F2 clones were the most responsive for shoot meristem in vitro culture. WPM medium supplemented with 8.8 µM 2iP, 0.2 µM NAA and 3 g L-1 activated charcoal was the most suitable for the in vitro establishment of the F1 clone. No phytotoxic effect of the disinfectant was observed and some meristems sprouted. The isolated endophytic bacterium was identified for the first time in yerba mate as Agrobacterium larrymoorei. To conclude, we were able to establish in vitro culture of yerba mate using meristems as explants but the tissues were not free of endophytic microorganisms which could interfere with explant response. |
Palavras-Chave: |
2iP; Activated charcoal; Endophytic bacteria; Erva mate. |
Thesagro: |
Cultura de Tecido; Ilex Paraguariensis; Oxidação. |
Thesaurus Nal: |
Agrobacterium larrymoorei; Oxidation; Tissue culture; Yerba mate. |
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
Marc: |
LEADER 02375naa a2200349 a 4500 001 2114253 005 2019-11-13 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1007/s11676-018-0763-x$2DOI 100 1 $aTOMASI, J. de C. 245 $aIn vitro establishment of shoot meristems of Ilex paraguariensis and identification of endophytic bacteria.$h[electronic resource] 260 $c2019 520 $aYerba mate (Ilex paraguariensis A. St.-Hil.) is a species of great economic, social and environmental importance for the southern regions of Brazil, Uruguay and Argentina. Currently the most diverse products are obtained from mate leaves, including mate tea. The objective of this study was to establish shoot meristem cultures (meristematic dome and a few primordia) of elite clones and identify the endophytic bacteria present in the explants. We tested the effect of clones (F1, F2, A03 and A07), culture media (MS, 1/2MS, 1/4MS and WPM), cytokinins (kinetin, BA and 2iP), activated charcoal (1, 2 and 3 g L-1), and disinfecting agent (sodium hypochlorite and mercuric chloride) on in vitro establishment. F1 and F2 clones were the most responsive for shoot meristem in vitro culture. WPM medium supplemented with 8.8 µM 2iP, 0.2 µM NAA and 3 g L-1 activated charcoal was the most suitable for the in vitro establishment of the F1 clone. No phytotoxic effect of the disinfectant was observed and some meristems sprouted. The isolated endophytic bacterium was identified for the first time in yerba mate as Agrobacterium larrymoorei. To conclude, we were able to establish in vitro culture of yerba mate using meristems as explants but the tissues were not free of endophytic microorganisms which could interfere with explant response. 650 $aAgrobacterium larrymoorei 650 $aOxidation 650 $aTissue culture 650 $aYerba mate 650 $aCultura de Tecido 650 $aIlex Paraguariensis 650 $aOxidação 653 $a2iP 653 $aActivated charcoal 653 $aEndophytic bacteria 653 $aErva mate 700 1 $aDEGENHARDT-GOLDBACH, J. 700 1 $aGRUNENNVALDT, R. L. 700 1 $aSANTOS, G. D. dos 700 1 $aVICENTE, V. A. 700 1 $aFRANCISCON, L. 700 1 $aBONA, C. 700 1 $aQUOIRIN, M. 773 $tJournal of Forestry Research$gv. 30, n. 5, p. 1765-1777, Oct. 2019.
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3. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | DEDECEK, R. A. Manejo de solos em ervais. In: CONGRESSO SUL-AMERICANO DA ERVA-MATE, 1.; REUNIAO TECNICA DO CONE SUL SOBRE A CULTURA DA ERVA-MATE, 2., 1997, Curitiba. Anais. Colombo: EMBRAPA-CNPF, 1997. p. 317-336. (EMBRAPA-CNPF. Documentos, 33).Biblioteca(s): Embrapa Florestas. |
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5. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | DEDECEK, R. A. A dinamica dos solos em areas degradadas. In: SIMPOSIO NACIONAL RECUPERACAO DE AREAS DEGRADADAS, 1992, Curitiba. [Anais...]. Curitiba: Universidade Federal do Parana. Curso de Engenharia Florestal. Departamento de Silvicultura e Manejo / FUPEF, 1992. p.44-57.Biblioteca(s): Embrapa Florestas. |
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10. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | DEDECEK, R. A.; FIER, I. S. N. Influência do sítio no desenvolvimento de Pinus taeda. In: CONGRESSO FLORESTAL BRASILEIRO, 8., 2003, São Paulo. Benefícios, Produtos e Serviços da Floresta: Oportunidades e Desafios do Século XX. São Paulo: Sociedade Brasileira de Silvicultura: Sociedade Brasileira de Engenheiros Florestais, 2003. CD-ROM. Resumo.Tipo: Resumo em Anais de Congresso |
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20. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | PHILIPOVSKY, J. F.; MEDRADO, M. J. S.; DEDECEK, R. A. Avaliacao de coberturas verdes de inverno em solos cultivados com erva-mate, nos municipios de Aurea-RS, Ivai-PR e Ponta Grossa-PR. In: CONGRESSO SUL-AMERICANO DA ERVA-MATE, 1.; REUNIAO TECNICA DO CONE SUL SOBRE A CULTURA DA ERVA-MATE, 2., 1997, Curitiba. Anais. Colombo: EMBRAPA-CNPF, 1997. p.448.Biblioteca(s): Embrapa Florestas. |
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