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Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
09/04/2015 |
Data da última atualização: |
09/04/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ERBANO, M.; SCHUHLI, G. S. e; SANTOS, É. P. dos. |
Afiliação: |
Marianna Erbano, Pós-graduanda da UFPR; GUILHERME SCHNELL E SCHUHLI, CNPF; Élide Pereira dos Santos, UFPR. |
Título: |
Genetic variability and population structure of Salvia lachnostachys: implications for breeding and conservation programs. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
International Journal of Molecular Sciences, v. 16, p. 7839-7850, 2015. |
DOI: |
10.3390/ijms16047839 |
Idioma: |
Inglês |
Conteúdo: |
The genetic diversity and population structure of Salvia lachnostachys Benth were assessed. Inter Simple Sequence Repeat (ISSR) molecular markers were used to investigate the restricted distribution of S. lachnostachys in Parana State, Brazil. Leaves of 73 individuals representing three populations were collected. DNA was extracted and submitted to PCR-ISSR amplification with nine tested primers. Genetic diversity parameters were evaluated. Our analysis indicated 95.6% polymorphic loci (stress value 0.02) with a 0.79 average Simpson?s index. The Nei-Li distance dendrogram and principal component analysis largely recovered the geographical origin of each sample. Four major clusters were recognized representing each collected population. Nei?s gene diversity and Shannon?s information index were 0.25 and 0.40 respectively. As is typical for outcrossing herbs, the majority of genetic variation occurred at the population level (81.76%). A high gene flow (Nm = 2.48) was observed with a correspondingly low fixation index. These values were generally similar to previous studies on congeneric species. The results of principal coordinate analysis (PCA) and of arithmetic average (UPGMA) were consistent and all three populations appear distinct as in STRUCTURE analysis. In addition, this analysis indicated a majority intrapopulation genetic variation. Despite the human pressure on natural populations our study found high levels of genetic diversity for S. lachnostachys. This was the first molecular assessment for this endemic species with medicinal proprieties and the results can guide for subsequent bioprospection, breeding programs or conservation actions. MenosThe genetic diversity and population structure of Salvia lachnostachys Benth were assessed. Inter Simple Sequence Repeat (ISSR) molecular markers were used to investigate the restricted distribution of S. lachnostachys in Parana State, Brazil. Leaves of 73 individuals representing three populations were collected. DNA was extracted and submitted to PCR-ISSR amplification with nine tested primers. Genetic diversity parameters were evaluated. Our analysis indicated 95.6% polymorphic loci (stress value 0.02) with a 0.79 average Simpson?s index. The Nei-Li distance dendrogram and principal component analysis largely recovered the geographical origin of each sample. Four major clusters were recognized representing each collected population. Nei?s gene diversity and Shannon?s information index were 0.25 and 0.40 respectively. As is typical for outcrossing herbs, the majority of genetic variation occurred at the population level (81.76%). A high gene flow (Nm = 2.48) was observed with a correspondingly low fixation index. These values were generally similar to previous studies on congeneric species. The results of principal coordinate analysis (PCA) and of arithmetic average (UPGMA) were consistent and all three populations appear distinct as in STRUCTURE analysis. In addition, this analysis indicated a majority intrapopulation genetic variation. Despite the human pressure on natural populations our study found high levels of genetic diversity for S. lachnostachys. This was the fir... Mostrar Tudo |
Palavras-Chave: |
Estrutura populacional; Intraspecific diversity; ISSR; Labiatae; Melhoramento genético; Salvia lachnostachys; Variabilidade genética. |
Thesagro: |
Conservação. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/122031/1/2015-API-GuilhermeS-GeneticVariability.pdf
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Marc: |
LEADER 02492naa a2200253 a 4500 001 2013073 005 2015-04-09 008 2015 bl uuuu u00u1 u #d 024 7 $a10.3390/ijms16047839$2DOI 100 1 $aERBANO, M. 245 $aGenetic variability and population structure of Salvia lachnostachys$bimplications for breeding and conservation programs.$h[electronic resource] 260 $c2015 520 $aThe genetic diversity and population structure of Salvia lachnostachys Benth were assessed. Inter Simple Sequence Repeat (ISSR) molecular markers were used to investigate the restricted distribution of S. lachnostachys in Parana State, Brazil. Leaves of 73 individuals representing three populations were collected. DNA was extracted and submitted to PCR-ISSR amplification with nine tested primers. Genetic diversity parameters were evaluated. Our analysis indicated 95.6% polymorphic loci (stress value 0.02) with a 0.79 average Simpson?s index. The Nei-Li distance dendrogram and principal component analysis largely recovered the geographical origin of each sample. Four major clusters were recognized representing each collected population. Nei?s gene diversity and Shannon?s information index were 0.25 and 0.40 respectively. As is typical for outcrossing herbs, the majority of genetic variation occurred at the population level (81.76%). A high gene flow (Nm = 2.48) was observed with a correspondingly low fixation index. These values were generally similar to previous studies on congeneric species. The results of principal coordinate analysis (PCA) and of arithmetic average (UPGMA) were consistent and all three populations appear distinct as in STRUCTURE analysis. In addition, this analysis indicated a majority intrapopulation genetic variation. Despite the human pressure on natural populations our study found high levels of genetic diversity for S. lachnostachys. This was the first molecular assessment for this endemic species with medicinal proprieties and the results can guide for subsequent bioprospection, breeding programs or conservation actions. 650 $aConservação 653 $aEstrutura populacional 653 $aIntraspecific diversity 653 $aISSR 653 $aLabiatae 653 $aMelhoramento genético 653 $aSalvia lachnostachys 653 $aVariabilidade genética 700 1 $aSCHUHLI, G. S. e 700 1 $aSANTOS, É. P. dos 773 $tInternational Journal of Molecular Sciences$gv. 16, p. 7839-7850, 2015.
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Registro original: |
Embrapa Florestas (CNPF) |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
11/08/2011 |
Data da última atualização: |
11/08/2011 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
ANDRADE, H. M.; VIANA, J. H. M.; DONAGEMMA, G. K.; PAZ, L. F. da; MELO, B. M. de. |
Afiliação: |
HOSANA MARIA ANDRADE, CNPMS; JOAO HERBERT MOREIRA VIANA, CNPMS; GUILHERME KANGUSSU DONAGEMMA, CNPS; LUCIMARA FERNANDA DA PAZ, BOLSISTA; BIANCA MATOS DE MELO, BOLSISTA. |
Título: |
Avaliação da repetitividade e reprodutibilidade para cálculo de incerteza na análise granulométrica. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 33., 2011, Uberlândia. Solos nos biomas brasileiros: sustentabilidade e mudanças climáticas. Uberlândia: Sociedade Brasileira de Ciência do Solo, 2011. |
Descrição Física: |
1 CD-ROM. |
Idioma: |
Português |
Palavras-Chave: |
Análise de erro; Conteúdo de argila. |
Thesagro: |
Análise do solo; Controle de Qualidade; Solo argiloso. |
Thesaurus NAL: |
Clay soils; Soil analysis. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/39692/1/Avaliacao-repetitividade.pdf
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Marc: |
LEADER 00895nam a2200241 a 4500 001 1897814 005 2011-08-11 008 2011 bl uuuu u00u1 u #d 100 1 $aANDRADE, H. M. 245 $aAvaliação da repetitividade e reprodutibilidade para cálculo de incerteza na análise granulométrica.$h[electronic resource] 260 $aIn: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 33., 2011, Uberlândia. Solos nos biomas brasileiros: sustentabilidade e mudanças climáticas. Uberlândia: Sociedade Brasileira de Ciência do Solo$c2011 300 $c1 CD-ROM. 650 $aClay soils 650 $aSoil analysis 650 $aAnálise do solo 650 $aControle de Qualidade 650 $aSolo argiloso 653 $aAnálise de erro 653 $aConteúdo de argila 700 1 $aVIANA, J. H. M. 700 1 $aDONAGEMMA, G. K. 700 1 $aPAZ, L. F. da 700 1 $aMELO, B. M. de
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