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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
02/10/2008 |
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Data da última atualização: |
02/10/2008 |
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Autoria: |
SANTOS, E. M. R.; FRANKLIN, E.; LUIZÃO, F. J. |
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Título: |
Litter manipulation and associated invertebrate fauna in secondary forest, central Amazonia, Brazil. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM. |
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Idioma: |
Inglês |
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Conteúdo: |
Plant litter from selected tree species have been used for improving soil productivity in low-input
systems of secondary vegetation in central Amazon, leading to different conditions for the soil
organisms. Soil animal assemblages were monitored to test the effects of adding litter types of
contrasting nutritional quality, and periods of exposure on the development of the invertebrate
community. We established four second growth plots with 80 subplots of 3 m2 from which the
original litter was removed and replaced in 60 subplots. Twenty subplots received Hevea
brasiliensis leaves, 20 others Carapa guianensis leaves, and another 20 an equal mixture of H.
brasiliensis, C. guianensis and Vismia guianensis. Twenty subplots were left with the original
litter. Litter and mineral soil (5 cm deep) sub-horizons were collected after 45, 100, 160, 240
and 300 days of exposure. The invertebrates were extracted using Kempson apparatus. At the
day 210th, the litter was replenished to match the surrounding litter. Regression analyses showed
no significant effect of litter type, but the period of exposure did affect the community in both subhorizons.
Only after the litter replacement, the type of litter and periods of exposure affected the
community in the litter sub-horizon. Because we tried to isolate the effects of litter composition
from other large-scale phenomena, several factors interfered in the experiment and potential
problems were identified to optimize the investigation. The results suggest that the sampling
design must be improved by using a larger number of subsamples for each kind of litter within
each plot. As the scale of the experiments was too small, future manipulations should be done
on much larger scale so that their effects on ecosystem processes can be evaluated, and the
replicates need to be spread over larger areas to capture the natural variations within ecosystems.
Financial support: SHIFT/ENV 052, CNPq/PNOPG: 55 04 09/01-7, CAPES. MenosPlant litter from selected tree species have been used for improving soil productivity in low-input
systems of secondary vegetation in central Amazon, leading to different conditions for the soil
organisms. Soil animal assemblages were monitored to test the effects of adding litter types of
contrasting nutritional quality, and periods of exposure on the development of the invertebrate
community. We established four second growth plots with 80 subplots of 3 m2 from which the
original litter was removed and replaced in 60 subplots. Twenty subplots received Hevea
brasiliensis leaves, 20 others Carapa guianensis leaves, and another 20 an equal mixture of H.
brasiliensis, C. guianensis and Vismia guianensis. Twenty subplots were left with the original
litter. Litter and mineral soil (5 cm deep) sub-horizons were collected after 45, 100, 160, 240
and 300 days of exposure. The invertebrates were extracted using Kempson apparatus. At the
day 210th, the litter was replenished to match the surrounding litter. Regression analyses showed
no significant effect of litter type, but the period of exposure did affect the community in both subhorizons.
Only after the litter replacement, the type of litter and periods of exposure affected the
community in the litter sub-horizon. Because we tried to isolate the effects of litter composition
from other large-scale phenomena, several factors interfered in the experiment and potential
problems were identified to optimize the investigation. The res... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02680naa a2200145 a 4500
001 1315004
005 2008-10-02
008 2008 bl uuuu u00u1 u #d
100 1 $aSANTOS, E. M. R.
245 $aLitter manipulation and associated invertebrate fauna in secondary forest, central Amazonia, Brazil.
260 $c2008
520 $aPlant litter from selected tree species have been used for improving soil productivity in low-input systems of secondary vegetation in central Amazon, leading to different conditions for the soil organisms. Soil animal assemblages were monitored to test the effects of adding litter types of contrasting nutritional quality, and periods of exposure on the development of the invertebrate community. We established four second growth plots with 80 subplots of 3 m2 from which the original litter was removed and replaced in 60 subplots. Twenty subplots received Hevea brasiliensis leaves, 20 others Carapa guianensis leaves, and another 20 an equal mixture of H. brasiliensis, C. guianensis and Vismia guianensis. Twenty subplots were left with the original litter. Litter and mineral soil (5 cm deep) sub-horizons were collected after 45, 100, 160, 240 and 300 days of exposure. The invertebrates were extracted using Kempson apparatus. At the day 210th, the litter was replenished to match the surrounding litter. Regression analyses showed no significant effect of litter type, but the period of exposure did affect the community in both subhorizons. Only after the litter replacement, the type of litter and periods of exposure affected the community in the litter sub-horizon. Because we tried to isolate the effects of litter composition from other large-scale phenomena, several factors interfered in the experiment and potential problems were identified to optimize the investigation. The results suggest that the sampling design must be improved by using a larger number of subsamples for each kind of litter within each plot. As the scale of the experiments was too small, future manipulations should be done on much larger scale so that their effects on ecosystem processes can be evaluated, and the replicates need to be spread over larger areas to capture the natural variations within ecosystems. Financial support: SHIFT/ENV 052, CNPq/PNOPG: 55 04 09/01-7, CAPES.
700 1 $aFRANKLIN, E.
700 1 $aLUIZÃO, F. J.
773 $tIn: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM.
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Registro original: |
Embrapa Florestas (CNPF) |
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Biblioteca |
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Origem |
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Classificação |
Cutter |
Registro |
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URL |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
31/03/2008 |
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Data da última atualização: |
26/09/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
PACHECO, L. G. C.; PENA, R. R.; CASTRO, T. L. P.; DORELLA, F. A.; BAHIA, R. C.; CARMINATI, R.; FROTA, M. N. L.; OLIVEIRA, S. C.; MEYER, R.; ALVES, F. S. F.; MIYOSHI, A.; AZEVEDO, V. |
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Afiliação: |
Luis G. C. Pacheco, Instituto de Ciências Biológicas UFMG; Roberta R. Pena, Instituto de Ciências Biológicas UFMG; Thiago L. P. Castro Instituto de Ciências Biológicas UFMG; Fernanda A. Dorella Instituto de Ciências Biológicas UFMG; Robson C. Bahia, UFBA-Departamento de Bio-Interação; Renato Carminati, UFBA-Departamento de Bio-Interação; Maurício N. L. Frota, UVA - Universidade Estadual Vale do Acaraú; Sérgio C. Oliveira, Instituto de Ciências Biológicas UFMG; Roberto Meyer, UFBA-Departamento de Bio-Interação; FRANCISCO SELMO FERNANDES ALVES, CNPC; Anderson Miyoshi, Instituto de Ciências Biológicas UFMG; Vasco Azevedo, Instituto de Ciências Biológicas UFMG. |
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Título: |
Multiplex PCR assay for identification of Corynebacterium pseudotuberculosis from pure cultures and for rapid detection of this pathogen in clinical samples. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Journal of Medical Microbiology, v. 56, n. 4, p. 480-486, Apr. 2007. |
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DOI: |
10.1099/jmm.0.46997-0 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Corynebacterium pseudotuberculosis is the aetiological agent of caseous lymphadenitis (CLA), a debilitating disease of sheep and goats. Accurate diagnosis of CLA primarily relies on microbiological examination, followed by biochemical identification of isolates. In an effort to facilitate C. pseudotuberculosis detection, a multiplex PCR (mPCR) assay was developed targeting three genes of this bacterium: the 16S rRNA gene, rpoB and pld. This method allowed efficient identification of 40 isolates of this bacterium that had been identified previously by biochemical testing. Analysis of taxonomically related species did not generate the C. pseudotuberculosis mPCR amplification profile, thereby demonstrating the assay's specificity. As little as 1 pg of C. pseudotuberculosis genomic DNA was detected by this mPCR assay, demonstrating the sensitivity of the method. The detection limit in clinical samples was estimated to be 103 c.f.u. C. pseudotuberculosis could be detected directly in pus samples from infected sheep and goats (n=56) with a high diagnostic sensitivity (94.6 %). The developed assay significantly improves rapid C. pseudotuberculosis detection and could supersede bacteriological culture for microbiological and epidemiological diagnosis of CLA. |
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Palavras-Chave: |
CLA; PCR. |
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Thesagro: |
Caprino; Corynebacterium Pseudotuberculosis; Linfadenite Caseosa; Ovino. |
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Thesaurus NAL: |
caseous lymphadenitis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/85860/1/API-Multiplex-PCR-assay.pdf
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Marc: |
LEADER 02339naa a2200349 a 4500
001 1533624
005 2019-09-26
008 2007 bl uuuu u00u1 u #d
024 7 $a10.1099/jmm.0.46997-0$2DOI
100 1 $aPACHECO, L. G. C.
245 $aMultiplex PCR assay for identification of Corynebacterium pseudotuberculosis from pure cultures and for rapid detection of this pathogen in clinical samples.$h[electronic resource]
260 $c2007
520 $aAbstract: Corynebacterium pseudotuberculosis is the aetiological agent of caseous lymphadenitis (CLA), a debilitating disease of sheep and goats. Accurate diagnosis of CLA primarily relies on microbiological examination, followed by biochemical identification of isolates. In an effort to facilitate C. pseudotuberculosis detection, a multiplex PCR (mPCR) assay was developed targeting three genes of this bacterium: the 16S rRNA gene, rpoB and pld. This method allowed efficient identification of 40 isolates of this bacterium that had been identified previously by biochemical testing. Analysis of taxonomically related species did not generate the C. pseudotuberculosis mPCR amplification profile, thereby demonstrating the assay's specificity. As little as 1 pg of C. pseudotuberculosis genomic DNA was detected by this mPCR assay, demonstrating the sensitivity of the method. The detection limit in clinical samples was estimated to be 103 c.f.u. C. pseudotuberculosis could be detected directly in pus samples from infected sheep and goats (n=56) with a high diagnostic sensitivity (94.6 %). The developed assay significantly improves rapid C. pseudotuberculosis detection and could supersede bacteriological culture for microbiological and epidemiological diagnosis of CLA.
650 $acaseous lymphadenitis
650 $aCaprino
650 $aCorynebacterium Pseudotuberculosis
650 $aLinfadenite Caseosa
650 $aOvino
653 $aCLA
653 $aPCR
700 1 $aPENA, R. R.
700 1 $aCASTRO, T. L. P.
700 1 $aDORELLA, F. A.
700 1 $aBAHIA, R. C.
700 1 $aCARMINATI, R.
700 1 $aFROTA, M. N. L.
700 1 $aOLIVEIRA, S. C.
700 1 $aMEYER, R.
700 1 $aALVES, F. S. F.
700 1 $aMIYOSHI, A.
700 1 $aAZEVEDO, V.
773 $tJournal of Medical Microbiology$gv. 56, n. 4, p. 480-486, Apr. 2007.
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Embrapa Caprinos e Ovinos (CNPC) |
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