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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
04/10/2016 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
SOUSA, P. C.; SANTOS, E. A. A.; SILVA, A. M.; BEZERRA, J. A. B.; SOUZA, A. L. P.; LIMA, G. L.; OLIVEIRA, M. F.; SILVA, A. R. |
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Afiliação: |
PATRÍCIA C. SOUSA, UFERSA; ERIKA A. A. SANTOS, UFERSA; ANDRÉIA M. SILVA, UFERSA; JOSÉ A. B. BEZERRA, UFERSA; ANA L. P. SOUZA, UFERSA; GABRIELA L. LIMA, UFRR; MOACIR F. OLIVEIRA, UFERSA; ALEXANDRE R. SILVA, UFERSA. |
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Título: |
Identification of ultrastructural and functional damage in sperm from six-banded armadillos (Euphractus sexcinctus) due to cryopreservation. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 8, p. 767-774, ago. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of the study was to cryopreserve the semen of six-banded armadillos (Euphractus sexcinctus) in Tris-yolk and glycerol diluent, and to determine the damage caused by the freezing-thawing process, using fluorescent markers and ultrastructural analysis. Semen samples (n=11) collected from 4 adult six-banded armadillos by electroejaculation were cryopreserved in Tris diluent plus 20% egg yolk and 3% glycerol, in a fast freezing curve. Classical analysis of samples was performed after dilution, refrigeration and thawing, followed by fluorescence analysis, using a combination of fluorescent probes to assess membrane integrity (propidium iodide - PI and Hoechst - H342), and mitochondrial activity (CMXRos - Mito Tracker Red®). We also used the ultrastructural analysis to verify possible morphological alterations caused by cryoinjuries. When compared with fresh samples, we verified a significant decline in all the armadillos? semen parameters after thawing, in which only 6.1% motile sperm were found. However, the percentage of sperm which remained with viable (13%) and functional (24.7%) membranes after thawing suggests that some cells could be live but immotile. Analysis using fluorescent markers revealed that the mitochondria of armadillos? sperm is highly sensible to the freezing protocol and the findings through ultrastructure analysis proved this statement. Additionally, the images obtained by transmission electron microscopy revealed that frozenthawed sperm presented damaged plasma membrane, nuclear modifications as changes in chromatin and acrossomal changes relative to sperm capacitation. In conclusion, this study is the first attempt to cryopreserve the semen of an armadillo species, and to help us to identify critical points on the freezing-thawing procedure in order to improve the protocol. MenosThe aim of the study was to cryopreserve the semen of six-banded armadillos (Euphractus sexcinctus) in Tris-yolk and glycerol diluent, and to determine the damage caused by the freezing-thawing process, using fluorescent markers and ultrastructural analysis. Semen samples (n=11) collected from 4 adult six-banded armadillos by electroejaculation were cryopreserved in Tris diluent plus 20% egg yolk and 3% glycerol, in a fast freezing curve. Classical analysis of samples was performed after dilution, refrigeration and thawing, followed by fluorescence analysis, using a combination of fluorescent probes to assess membrane integrity (propidium iodide - PI and Hoechst - H342), and mitochondrial activity (CMXRos - Mito Tracker Red®). We also used the ultrastructural analysis to verify possible morphological alterations caused by cryoinjuries. When compared with fresh samples, we verified a significant decline in all the armadillos? semen parameters after thawing, in which only 6.1% motile sperm were found. However, the percentage of sperm which remained with viable (13%) and functional (24.7%) membranes after thawing suggests that some cells could be live but immotile. Analysis using fluorescent markers revealed that the mitochondria of armadillos? sperm is highly sensible to the freezing protocol and the findings through ultrastructure analysis proved this statement. Additionally, the images obtained by transmission electron microscopy revealed that frozenthawed sperm presented da... Mostrar Tudo |
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Palavras-Chave: |
Dano ultraestrutural e funcional; Euphractus sexcinctus; Membrane integrity; Mitochondrial activity; Six-banded armadillo; Sperm; Tatu-peba; Ultraestrutura; Ultrastructural and functional damages; Xenarthras. |
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Thesagro: |
Criopreservação; Espermatozoide; Membrana; Mitocondria; Sêmen. |
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Thesaurus Nal: |
Cryopreservation; Spermatozoa; Ultrastructure. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/148299/1/Identification-of-ultrastructural.pdf
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Marc: |
LEADER 03089naa a2200421 a 4500
001 2054060
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aSOUSA, P. C.
245 $aIdentification of ultrastructural and functional damage in sperm from six-banded armadillos (Euphractus sexcinctus) due to cryopreservation.
260 $c2016
520 $aThe aim of the study was to cryopreserve the semen of six-banded armadillos (Euphractus sexcinctus) in Tris-yolk and glycerol diluent, and to determine the damage caused by the freezing-thawing process, using fluorescent markers and ultrastructural analysis. Semen samples (n=11) collected from 4 adult six-banded armadillos by electroejaculation were cryopreserved in Tris diluent plus 20% egg yolk and 3% glycerol, in a fast freezing curve. Classical analysis of samples was performed after dilution, refrigeration and thawing, followed by fluorescence analysis, using a combination of fluorescent probes to assess membrane integrity (propidium iodide - PI and Hoechst - H342), and mitochondrial activity (CMXRos - Mito Tracker Red®). We also used the ultrastructural analysis to verify possible morphological alterations caused by cryoinjuries. When compared with fresh samples, we verified a significant decline in all the armadillos? semen parameters after thawing, in which only 6.1% motile sperm were found. However, the percentage of sperm which remained with viable (13%) and functional (24.7%) membranes after thawing suggests that some cells could be live but immotile. Analysis using fluorescent markers revealed that the mitochondria of armadillos? sperm is highly sensible to the freezing protocol and the findings through ultrastructure analysis proved this statement. Additionally, the images obtained by transmission electron microscopy revealed that frozenthawed sperm presented damaged plasma membrane, nuclear modifications as changes in chromatin and acrossomal changes relative to sperm capacitation. In conclusion, this study is the first attempt to cryopreserve the semen of an armadillo species, and to help us to identify critical points on the freezing-thawing procedure in order to improve the protocol.
650 $aCryopreservation
650 $aSpermatozoa
650 $aUltrastructure
650 $aCriopreservação
650 $aEspermatozoide
650 $aMembrana
650 $aMitocondria
650 $aSêmen
653 $aDano ultraestrutural e funcional
653 $aEuphractus sexcinctus
653 $aMembrane integrity
653 $aMitochondrial activity
653 $aSix-banded armadillo
653 $aSperm
653 $aTatu-peba
653 $aUltraestrutura
653 $aUltrastructural and functional damages
653 $aXenarthras
700 1 $aSANTOS, E. A. A.
700 1 $aSILVA, A. M.
700 1 $aBEZERRA, J. A. B.
700 1 $aSOUZA, A. L. P.
700 1 $aLIMA, G. L.
700 1 $aOLIVEIRA, M. F.
700 1 $aSILVA, A. R.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 8, p. 767-774, ago. 2016.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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| Registros recuperados : 2 | |
| 1. |  | SOUSA, P. C.; SANTOS, E. A. A.; SILVA, A. M.; BEZERRA, J. A. B.; SOUZA, A. L. P.; LIMA, G. L.; OLIVEIRA, M. F.; SILVA, A. R. Identification of ultrastructural and functional damage in sperm from six-banded armadillos (Euphractus sexcinctus) due to cryopreservation. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 8, p. 767-774, ago. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 2. | | SANTOS, E. A. A.; LIMA, G. L.; PRAXEDES, E. C. G.; SILVA, A. M.; MAIA, K. M.; OLIVEIRA, M. F.; RODRIGUES, A. P. R.; SILVA, A. R. Estimation, morphometry and ultrastructure of ovarian preantral follicle population in agouti (Dasyprocta leporina). Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 1, p. 175-182, janeiro 2018. Título em português: Morfometria, estimativa e ultraestrutura da população folicular ovariana preantral de cutia (Dasyprocta leporina).| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 2 | |
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