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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
14/03/2011 |
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Data da última atualização: |
14/03/2011 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
ALMEIDA, J. D. de; BARROS, L. M. G.; SANTOS, D. B. M.; COTTA, M. G.; BARBOSA, E. A.; CAÇÃO, S. B.; EIRA, M. T. S. da; ALVES, G. S. C.; VINECKY, F.; PEREIRA, L. F. P.; SILVA, F. R. da; ANDRADE, A. C.; MARRACCINI, P.; CARNEIRO, M. |
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Afiliação: |
JULIANA DANTAS DE ALMEIDA, CENARGEN; LEILA MARIA GOMES BARROS, CENARGEN; IAPAR; MIRIAN THEREZINHA SOUZA DA EIRA, SAPC; LUIZ FILIPE PROTASIO PEREIRA, SAPC; ALAN CARVALHO ANDRADE, CENARGEN; CIRAD UMR-DAP; MAURO CARNEIRO, CENARGEN. |
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Título: |
Prospection of tissue specific promoters in coffee. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22. 2008, Campinas, São Paulo, Brazil. |
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Idioma: |
Inglês |
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Conteúdo: |
The majority of transgenic organisms reported in the literature have been made using constitutive promoters. However, there are economic, environmental and biosecurity related restrictions involving indiscriminate (constitutive) expression of heterologous genes. The usage of tissue-specific and induced promoters can resolve those issues by limiting the expression of a transgene to the necessary tissues and conditions. The promoters currently used at Embrapa are transnational properties, burdening the research and causing technological dependence. Therefore, the objective of this work was to find and characterize tissue and organ specific promoter in Coffea spp. We have used the Coffee genome database in silico tools to find genes preferentially expressed in root, leaf and fruit. In this way we found 72 organ-specific candidates: 18 apparently preferentially expressed on leaves, 14 on roots and 40 on fruits. Some of those candidates were tested in vitro using RT-PCR, semiquantitative PCR, northern blotting and qPCR assays. All four leaf-specific candidates tested (GCFo1, GCFo2, GCFo3 and GCFo4) and at least one of the two fruit-specific candidates tested (GCFr1 e GCFr2) where confirmed to be preferentially expressed on their respective organs. Temporal and spatial expression assays showed that GCFr2 has its expression peak at the endosperm, 180 days after flowering. The highest expressed genes of leaf (GCFo3 and GCFo4) and fruit (GCFr2) were used as probes to isolate its respective promoter through a BAC libraries screening or using the Genome Walker Universal Kit (Clontech). Results concerning gene expression and the molecular characterization of these genes will be presented. MenosThe majority of transgenic organisms reported in the literature have been made using constitutive promoters. However, there are economic, environmental and biosecurity related restrictions involving indiscriminate (constitutive) expression of heterologous genes. The usage of tissue-specific and induced promoters can resolve those issues by limiting the expression of a transgene to the necessary tissues and conditions. The promoters currently used at Embrapa are transnational properties, burdening the research and causing technological dependence. Therefore, the objective of this work was to find and characterize tissue and organ specific promoter in Coffea spp. We have used the Coffee genome database in silico tools to find genes preferentially expressed in root, leaf and fruit. In this way we found 72 organ-specific candidates: 18 apparently preferentially expressed on leaves, 14 on roots and 40 on fruits. Some of those candidates were tested in vitro using RT-PCR, semiquantitative PCR, northern blotting and qPCR assays. All four leaf-specific candidates tested (GCFo1, GCFo2, GCFo3 and GCFo4) and at least one of the two fruit-specific candidates tested (GCFr1 e GCFr2) where confirmed to be preferentially expressed on their respective organs. Temporal and spatial expression assays showed that GCFr2 has its expression peak at the endosperm, 180 days after flowering. The highest expressed genes of leaf (GCFo3 and GCFo4) and fruit (GCFr2) were used as probes to isolate its resp... Mostrar Tudo |
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Palavras-Chave: |
Coffee. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02510nam a2200277 a 4500
001 1880640
005 2011-03-14
008 2008 bl uuuu u00u1 u #d
100 1 $aALMEIDA, J. D. de
245 $aProspection of tissue specific promoters in coffee.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22. 2008, Campinas, São Paulo, Brazil.$c2008
520 $aThe majority of transgenic organisms reported in the literature have been made using constitutive promoters. However, there are economic, environmental and biosecurity related restrictions involving indiscriminate (constitutive) expression of heterologous genes. The usage of tissue-specific and induced promoters can resolve those issues by limiting the expression of a transgene to the necessary tissues and conditions. The promoters currently used at Embrapa are transnational properties, burdening the research and causing technological dependence. Therefore, the objective of this work was to find and characterize tissue and organ specific promoter in Coffea spp. We have used the Coffee genome database in silico tools to find genes preferentially expressed in root, leaf and fruit. In this way we found 72 organ-specific candidates: 18 apparently preferentially expressed on leaves, 14 on roots and 40 on fruits. Some of those candidates were tested in vitro using RT-PCR, semiquantitative PCR, northern blotting and qPCR assays. All four leaf-specific candidates tested (GCFo1, GCFo2, GCFo3 and GCFo4) and at least one of the two fruit-specific candidates tested (GCFr1 e GCFr2) where confirmed to be preferentially expressed on their respective organs. Temporal and spatial expression assays showed that GCFr2 has its expression peak at the endosperm, 180 days after flowering. The highest expressed genes of leaf (GCFo3 and GCFo4) and fruit (GCFr2) were used as probes to isolate its respective promoter through a BAC libraries screening or using the Genome Walker Universal Kit (Clontech). Results concerning gene expression and the molecular characterization of these genes will be presented.
653 $aCoffee
700 1 $aBARROS, L. M. G.
700 1 $aSANTOS, D. B. M.
700 1 $aCOTTA, M. G.
700 1 $aBARBOSA, E. A.
700 1 $aCAÇÃO, S. B.
700 1 $aEIRA, M. T. S. da
700 1 $aALVES, G. S. C.
700 1 $aVINECKY, F.
700 1 $aPEREIRA, L. F. P.
700 1 $aSILVA, F. R. da
700 1 $aANDRADE, A. C.
700 1 $aMARRACCINI, P.
700 1 $aCARNEIRO, M.
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Registro original: |
Embrapa Café (CNPCa) |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio-Norte; Embrapa Milho e Sorgo; Embrapa Rondônia. |
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Data corrente: |
10/09/2012 |
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Data da última atualização: |
13/09/2012 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
TARDIN, F. D.; ALMEIDA FILHO, J. E. de; DAHER, R. F.; MENEZES, C. B. de; SILVA, K. J. da; PAULA, C. M. de; XAVIER NETO, J. B.; ALBUQUERQUE, C. J. B.; BASTOS, E. A.; CARDOSO, M. J.; GODINHO, V. de P. C.; ANDRADE, F. F.; LOPES, V. da S.; SCHAFFERT, R. E. |
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Afiliação: |
FLAVIO DESSAUNE TARDIN, CNPMS; JANEO EUSTÁQUIO DE ALMEIDA FILHO, BOLSISTA; ROGÉRIO FIGUEIREDO DAHER, BOLSISTA; CICERO BESERRA DE MENEZES, CNPMS; KARLA JORGE DA SILVA, UFSJ; CÁSSIA MOABE DE PAULA, Agronoma; JOÃO BATISTA XAVIER NETO, Agronomo; CARLOS JULIANO BRANT ALBUQUERQUE, EPAMIG; EDSON ALVES BASTOS, CPAMN; MILTON JOSE CARDOSO, CPAMN; VICENTE DE PAULO CAMPOS GODINHO, CPAF-RO; FERNANDO FERNANDEZ ANDRADE, Agromen Sementes Agrícolas Ltda.; VINÍCIUS DA SILVA LOPES, Instituto Luterano de Ensino Superior de Itumbiara; ROBERT EUGENE SCHAFFERT, CNPMS. |
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Título: |
Desempenho agronômico de híbridos de sorgo granífero cultivados em vários ambientes brasileiros. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE MILHO E SORGO, 29., 2012, Águas de Lindóia. Diversidade e inovações na era dos transgênicos: resumos expandidos. Campinas: Instituto Agronômico; Sete Lagoas: Associação Brasileira de Milho e Sorgo, 2012. |
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Páginas: |
p. 3141-3147. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Palavras-Chave: |
Melhoramento genético; Rendimento de grão. |
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Thesagro: |
Safrinha; Sorghum Bicolor. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/66128/1/Desempenho-agronomico-2.pdf
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Marc: |
LEADER 01129nam a2200313 a 4500
001 1933247
005 2012-09-13
008 2012 bl uuuu u00u1 u #d
100 1 $aTARDIN, F. D.
245 $aDesempenho agronômico de híbridos de sorgo granífero cultivados em vários ambientes brasileiros.
260 $aIn: CONGRESSO NACIONAL DE MILHO E SORGO, 29., 2012, Águas de Lindóia. Diversidade e inovações na era dos transgênicos: resumos expandidos. Campinas: Instituto Agronômico; Sete Lagoas: Associação Brasileira de Milho e Sorgo$c2012
300 $ap. 3141-3147.$c1 CD-ROM.
650 $aSafrinha
650 $aSorghum Bicolor
653 $aMelhoramento genético
653 $aRendimento de grão
700 1 $aALMEIDA FILHO, J. E. de
700 1 $aDAHER, R. F.
700 1 $aMENEZES, C. B. de
700 1 $aSILVA, K. J. da
700 1 $aPAULA, C. M. de
700 1 $aXAVIER NETO, J. B.
700 1 $aALBUQUERQUE, C. J. B.
700 1 $aBASTOS, E. A.
700 1 $aCARDOSO, M. J.
700 1 $aGODINHO, V. de P. C.
700 1 $aANDRADE, F. F.
700 1 $aLOPES, V. da S.
700 1 $aSCHAFFERT, R. E.
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Embrapa Milho e Sorgo (CNPMS) |
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