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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
10/11/2017 |
Data da última atualização: |
17/04/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SALGADO, L. R.; LIMA, R.; SANTOS, B. F. dos; SHIRAKAWA, K. T.; VILELA, M. de M.; ALMEIDA, N. F.; PEREIRA, R. M.; NEPOMUCENO, A. L.; CHIARI, L. |
Afiliação: |
LEONARDO RIPPEL SALGADO, Embrapa Beef Cattle, Campo Grande, Mato Grosso do Sul, Brazil; RODOLPHO LIMA, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul, Brazil; BRUNO FERREIRA DOS SANTOS, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil; KARINA TAMIE SHIRAKAWA, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul, Brazil; MARIANE DE MENDONCA VILELA, CNPGC; NALVO FRANCO ALMEIDA, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil; RODRIGO MATHEUS PEREIRA, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul, Brazil; ALEXANDRE LIMA NEPOMUCENO, CNPSO; LUCIMARA CHIARI, CNPGC. |
Título: |
De novo RNA sequencing and analysis of the transcriptome of signalgrass (Urochloa decumbens) roots exposed to aluminum. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Plant Growth Regulation, v. 83, n. 1, p. 157-170, 2017. |
DOI: |
10.1007/s10725-017-0291-2 |
Idioma: |
Inglês |
Conteúdo: |
Acidic soils occupy a vast area in the world, and the aluminum (Al) in these soils can directly interact with plant cells and tissues to inhibit their growth and reduce yields. The signalgrass Urochloa decumbens cv. Basilisk (syn. Brachiaria decumbens cv. Basilisk), a widely sown tropical forage grass, is recognized for its high productivity under intensive use, vigorous growth, ease of establishment, and good forage value throughout the year, as well as its exceptional adaptation to infertile acid soils. We sequenced the transcriptome from roots of U. decumbens cv. Basilisk under two conditions, with and without Al, using Illumina paired-end sequencing technology and performed de novo assembly of those reads, which yielded 164,920 transcripts. Of these transcripts, 113,918 were assigned a putative protein function through comparisons with different gene set databases. Additionally, 13,375 simple sequence repeat (SSR) markers were identified. Digital gene expression analyses were conducted to identify 6698 differentially expressed genes between treatments, revealing a great differences in the root transcriptional landscape when exposed to aluminum. An extensive annotation of the differentially expressed genes (DEGs), made possible to identify several transcripts with putative functions correlated to aluminum exposure, most belonging to vesicle transportation, cell wall modifications and metal handling ontologies. In this work, abundant, high-quality transcripts were obtained, providing a reference platform for future biotechnological studies and breeding programs for this species and its close relatives. MenosAcidic soils occupy a vast area in the world, and the aluminum (Al) in these soils can directly interact with plant cells and tissues to inhibit their growth and reduce yields. The signalgrass Urochloa decumbens cv. Basilisk (syn. Brachiaria decumbens cv. Basilisk), a widely sown tropical forage grass, is recognized for its high productivity under intensive use, vigorous growth, ease of establishment, and good forage value throughout the year, as well as its exceptional adaptation to infertile acid soils. We sequenced the transcriptome from roots of U. decumbens cv. Basilisk under two conditions, with and without Al, using Illumina paired-end sequencing technology and performed de novo assembly of those reads, which yielded 164,920 transcripts. Of these transcripts, 113,918 were assigned a putative protein function through comparisons with different gene set databases. Additionally, 13,375 simple sequence repeat (SSR) markers were identified. Digital gene expression analyses were conducted to identify 6698 differentially expressed genes between treatments, revealing a great differences in the root transcriptional landscape when exposed to aluminum. An extensive annotation of the differentially expressed genes (DEGs), made possible to identify several transcripts with putative functions correlated to aluminum exposure, most belonging to vesicle transportation, cell wall modifications and metal handling ontologies. In this work, abundant, high-quality transcripts were obtained... Mostrar Tudo |
Palavras-Chave: |
Molecular markers; RNA sequencing; Urochloa genus. |
Thesagro: |
Brachiaria Decumbens. |
Thesaurus Nal: |
Aluminum. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166583/1/De-novo-RNA-sequencing-and-analysis.pdf
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Marc: |
LEADER 02506naa a2200289 a 4500 001 2079489 005 2018-04-17 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1007/s10725-017-0291-2$2DOI 100 1 $aSALGADO, L. R. 245 $aDe novo RNA sequencing and analysis of the transcriptome of signalgrass (Urochloa decumbens) roots exposed to aluminum.$h[electronic resource] 260 $c2017 520 $aAcidic soils occupy a vast area in the world, and the aluminum (Al) in these soils can directly interact with plant cells and tissues to inhibit their growth and reduce yields. The signalgrass Urochloa decumbens cv. Basilisk (syn. Brachiaria decumbens cv. Basilisk), a widely sown tropical forage grass, is recognized for its high productivity under intensive use, vigorous growth, ease of establishment, and good forage value throughout the year, as well as its exceptional adaptation to infertile acid soils. We sequenced the transcriptome from roots of U. decumbens cv. Basilisk under two conditions, with and without Al, using Illumina paired-end sequencing technology and performed de novo assembly of those reads, which yielded 164,920 transcripts. Of these transcripts, 113,918 were assigned a putative protein function through comparisons with different gene set databases. Additionally, 13,375 simple sequence repeat (SSR) markers were identified. Digital gene expression analyses were conducted to identify 6698 differentially expressed genes between treatments, revealing a great differences in the root transcriptional landscape when exposed to aluminum. An extensive annotation of the differentially expressed genes (DEGs), made possible to identify several transcripts with putative functions correlated to aluminum exposure, most belonging to vesicle transportation, cell wall modifications and metal handling ontologies. In this work, abundant, high-quality transcripts were obtained, providing a reference platform for future biotechnological studies and breeding programs for this species and its close relatives. 650 $aAluminum 650 $aBrachiaria Decumbens 653 $aMolecular markers 653 $aRNA sequencing 653 $aUrochloa genus 700 1 $aLIMA, R. 700 1 $aSANTOS, B. F. dos 700 1 $aSHIRAKAWA, K. T. 700 1 $aVILELA, M. de M. 700 1 $aALMEIDA, N. F. 700 1 $aPEREIRA, R. M. 700 1 $aNEPOMUCENO, A. L. 700 1 $aCHIARI, L. 773 $tPlant Growth Regulation$gv. 83, n. 1, p. 157-170, 2017.
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Embrapa Gado de Corte (CNPGC) |
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Registro Completo
Biblioteca(s): |
Embrapa Agroindústria Tropical. |
Data corrente: |
11/03/1994 |
Data da última atualização: |
23/06/2017 |
Autoria: |
MELO, Q. M. S.; CAVALCANTE, M. L. S.; MELO, F. I. O.; CAVALCANTE, R. D. |
Título: |
Incidência de pragas nos cajueirais do Ceara. |
Ano de publicação: |
1979 |
Fonte/Imprenta: |
Fortaleza: EPACE, 1979. |
Páginas: |
13 p. |
Série: |
(EPACE. Comunicado Técnico, 4). |
Idioma: |
Português |
Conteúdo: |
O cajueiro (Anacardium occidentale L.) tem se revelado bastante susceptivel ao ataque de diversos insetos e acaros. As informacoes existentes sobre a incidencia e severidade das diferentes pragas encontradas sao escassas. Esse tipo de conhecimento e entretanto de fundamental importancia na programacao de metodos de controle eficientes. A finalidade deste trabalho foi determinar a ocorrencia ou flutuacoes anual de diferentes pragas do cajueiro nas principais areas produtoras, como tambem, observar danos aparentes causados a cultura. |
Palavras-Chave: |
Acaro amarelo; Acaros; Aleurodiens cocois; Anthistarcha binocularis; Cerodirphia rubripes; Cicinnus callipius; Eacles imperiales magnifica; Lagarta das folhas; Lagarta saia justa; Lagarta verde; Tetraniquideo. |
Thesagro: |
Anacardium Occidentale; Aphis Gossypii; Besouro Vermelho; Broca das Pontas; Caju; Cochonilha; Crimissa Cruralis; Hospedeiro; Mosca Branca; Praga; Pulgão; Tripes. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01700nam a2200445 a 4500 001 1417955 005 2017-06-23 008 1979 bl uuuu u0uu1 u #d 100 1 $aMELO, Q. M. S. 245 $aIncidência de pragas nos cajueirais do Ceara. 260 $aFortaleza: EPACE$c1979 300 $a13 p. 490 $a(EPACE. Comunicado Técnico, 4). 520 $aO cajueiro (Anacardium occidentale L.) tem se revelado bastante susceptivel ao ataque de diversos insetos e acaros. As informacoes existentes sobre a incidencia e severidade das diferentes pragas encontradas sao escassas. Esse tipo de conhecimento e entretanto de fundamental importancia na programacao de metodos de controle eficientes. A finalidade deste trabalho foi determinar a ocorrencia ou flutuacoes anual de diferentes pragas do cajueiro nas principais areas produtoras, como tambem, observar danos aparentes causados a cultura. 650 $aAnacardium Occidentale 650 $aAphis Gossypii 650 $aBesouro Vermelho 650 $aBroca das Pontas 650 $aCaju 650 $aCochonilha 650 $aCrimissa Cruralis 650 $aHospedeiro 650 $aMosca Branca 650 $aPraga 650 $aPulgão 650 $aTripes 653 $aAcaro amarelo 653 $aAcaros 653 $aAleurodiens cocois 653 $aAnthistarcha binocularis 653 $aCerodirphia rubripes 653 $aCicinnus callipius 653 $aEacles imperiales magnifica 653 $aLagarta das folhas 653 $aLagarta saia justa 653 $aLagarta verde 653 $aTetraniquideo 700 1 $aCAVALCANTE, M. L. S. 700 1 $aMELO, F. I. O. 700 1 $aCAVALCANTE, R. D.
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