Data/hora: 23/09/2024 16:09:07

Biblioteca(s): 	Embrapa Amazônia Ocidental.	
Data corrente: 	30/05/2016	
Data da última atualização: 	30/05/2016	
Autoria: 	SANTOS, A. V. P. dos; DAVEY, M. R.; COCKING, E. C.	
Título: 	Cultural studies of Protoplasts and leaf Callus of Trigonella corniculata and T. foenum-graecum.	
Ano de publicação: 	1983	
Fonte/Imprenta: 	Zeitschrift für Pflanzenphysiologie, v. 109, n. 3, p. 227-234, Mar. 1983.	
DOI: 	doi:10.1016/S0044-328X(83)80224-4	
Idioma: 	Inglês	
Conteúdo: 	Mesophyll protoplasts were isolated using a Biogel-purified enzyme mixture from leaflets of vegetative and flowering stems of Trigonella corniculata and T. foenum-graecum grown in a controlled environment cabinet. Protoplasts of both species divided in liquid medium with a plating efficiency of 70%. Cell suspensions initiated from leaf callus of T. corniculata yielded protoplasts which formed callus. In contrast, protoplasts were not obtained from cell suspensions of T. foenum-graecum. Callus derived from leaf explants, mesophyll protoplasts and cell suspension protoplasts of T. corniculata formed embryoids and shoots, but tissues of T. foenum-graecum were recalcitrant to regeneration. These results, including the difficulties associated with T. foenum-graecum, are discussed in relation to recent progress on regeneration in forage legumes.	
Palavras-Chave: 	Embryoids; Genetic manipulation; Mesophyll protoplasts; Plating efficiency; Suspension cell protoplasts.	
Thesaurus Nal: 	forage legumes; Trigonella corniculata.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Amazônia Ocidental (CPAA)	
Exemplares: 	CPAA - (33580-1) - ADD - SP - PP - S6238 -  - S6238 - DIS