Data/hora: 22/06/2024 14:00:42

Biblioteca(s): 	Embrapa Unidades Centrais.	
Data corrente: 	19/03/2018	
Data da última atualização: 	19/03/2018	
Autoria: 	NOQUEIRA, R. de M. S.; SILVA, A. B.; SATO, T. P.; SÁ, J. C. de; SANTOS, A. C. G. dos; AMORIM FILHO, E. F.; VALE, T. L. do; GAZÊTA, G. S.	
Afiliação: 	Rita de Maria Seabra Nogueira, Departamento de Patologia/Universidade Estadual do Maranhão - UEMA; Arannadia Barbosa Silva, Laboratório de Referência Nacional em Vetores das Riquetsioses - LIRN/IOC/Fiocruz; Tayra Pereira Sato, Laboratório de Referência Nacional em Vetores das Riquetsioses - LIRN/IOC/Fiocruz; Joicy Cortez de Sá, Departamento de Patologia/Universidade Estadual do Maranhão - UEMA; Ana Clara Gomes dos Santos, Departamento de Patologia/Universidade Estadual do Maranhão - UEMA; Edvaldo Franco Amorim Filho, Departamento de Patologia/Universidade Estadual do Maranhão - UEMA; Tássia Lopes do Vale, Departamento de Patologia/Universidade Estadual do Maranhão - UEMA; Gilberto Salles Gazêta, Laboratório de Referência Nacional em Vetores das Riquetsioses - LIRN/IOC/Fiocruz.	
Título: 	Molecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil.	
Ano de publicação: 	2017	
Fonte/Imprenta: 	Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 12, p. 1416-1422, dezembro 2017.	
Idioma: 	Inglês	
Notas: 	Título em português: Detecção molecular e serológica de Theileria equi, Babesia caballi e Anaplasma phagocytophilum em equinos e carrapatos no Maranhão.	
Conteúdo: 	Equine piroplasmosis is a tick-borne disease caused by the intraeytrhocytic protozoans Babesia caballi and Theileria equi. It has been reported as a main equine parasitic disease. In addition, Anaplasma phagocytophilum, the causative agent of granulocytic ehrlichiosis, causes a seasonal disease in horses. Both diseases, can be detrimental to animal health. In this sense, blood samples and ticks were collected from 97 horses raised in the microregion of Baixada Maranhense, Maranhão State, Brazil. Serum samples were subjected to Indirect Fluorescence Antibody Test (IFAT) and blood samples and ticks to Polymerase Chain Reaction (PCR) to evaluate the infection by Theileria equi, Babesia caballi and Anaplasma phagocytophilum. The overall seroprevalence was 38.14%, 18.55% and 11.34% for T. equi, B. caballi and A. phagocytophilum, respectively. The results of PCR from blood samples showed 13.40% and 3.09% positive samples to T. equi and B. caballi, respectively. A total of 170 tick specimens were collected and identified as Dermacentor nitens, Amblyomma cajennense sensu lato and Rhipicephalus (Boophilus) microplus. It was detected 2.35% (4/170) and 0.59% (1/170) positive tick samples by PCR for T. equi and B. caballi, respectively. All samples were negative to A. phagocytophilum. No statically difference (p>0.05) was observed when gender, age, use of ectoparasiticide and tick presence were analyzed. A BLASTn analysis of the sequenced samples indicated 97 to 100% similarity with T. equi 18S rRNA gene sequences in GenBank and 98 to 100% with B. caballi. Genetic analysis classified the obtained sequences as T. equi and B. caballi cluster, respectively. It can be concluded that these pathogens occur and are circulating in the studied area.	
Palavras-Chave: 	Doença transmitida por vetores; Vector-borne disease.	
Thesagro: 	Carrapato; Cavalo.	
Thesaurus Nal: 	Anaplasma phagocytophilum; Babesia caballi; Brazil; Horses; Theileria equi; Ticks.	
Categoria do assunto: 	--	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/174112/1/Molecular-and-serological-detection-of-Theileria.pdf	
Registro original: 	Embrapa Unidades Centrais (AI-SEDE)	
Exemplares: 	AI-SEDE - (62305-1) - UPE - AP - DD -  -  -  - DIS