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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
21/06/2019 |
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Data da última atualização: |
18/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, W. R. de; PACHECO, T. F.; DUARTE, K. E.; SAMPAIO, B. L.; OLIVEIRA, P. A. de; MARTINS, P. M.; SANTIAGO, T. R.; FORMIGHIERI, E. F.; VINECKY, F.; RIBEIRO, A. P.; DIAS, B. B. A.; KOBAYASHI, A. K.; MITCHELL, R. A. C.; RODRIGUES, D. de S.; MOLINARI, H. B. C. |
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Afiliação: |
Wagner Rodrigo de Souza, Bolsista da Embrapa Agroenergia; THALYTA FRAGA PACHECO, CNPAE; Karoline Estefani Duarte, Bolsista da Embrapa Agroenergia; Bruno Leite Sampaio, Bolsista da Embrapa Agroenergia; PATRICIA ABRAO DE OLIVEIRA MOLINARI, CNPAE; Polyana Kelly Martins, Bolsista da Embrapa Agroenergia; Thaís Ribeiro Santiago, Bolsista da Embrapa Agroenergia; EDUARDO FERNANDES FORMIGHIERI, CNPAE; Felipe Vinecky; Ana Paula Ribeiro; BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; ADILSON KENJI KOBAYASHI, CNPAE; Rowan Andrew Craig Mitchell; DASCIANA DE SOUSA RODRIGUES, CNPAE; HUGO BRUNO CORREA MOLINARI, CNPAE. |
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Título: |
Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Biotechnology for Biofuels, v. 12, n. 111, p. 1-14, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Sugarcane (Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal candidate for improving composition of its residues (mostly cell walls), making them more suitable for cellulosic ethanol production. In this paper, we report an approach to improving saccharification of sugarcane straw by RNAi silencing of the recently discovered BAHD01 gene responsible for feruloylation of grass cell walls. Results: We identified six BAHD genes in the sugarcane genome (SacBAHDs) and generated five lines with substantially decreased SacBAHD01 expression. To find optimal conditions for determining saccharification of sugarcane straw, we tried multiple combinations of solvent and temperature pretreatment conditions, devising a predictive model for finding their effects on glucose release. Under optimal conditions, demonstrated by Organosolv pretreatment using 30% ethanol for 240 min, transgenic lines showed increases in saccharification efficiency of up to 24%. The three lines with improved saccharification efficiency had lower cell-wall ferulate content but unchanged monosaccharide and lignin compositions. Conclusions: The silencing of SacBAHD01 gene and subsequent decrease of cell-wall ferulate contents indicate a promising novel biotechnological approach for improving the suitability of sugarcane residues for cellulosic ethanol production. In addition, the Organosolv pretreatment of the genetically modified biomass and the optimal conditions for the enzymatic hydrolysis presented here might be incorporated in the sugarcane industry for bioethanol production. Keywords: Sugarcane, MenosBackground: Sugarcane (Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal candidate for improving composition of its residues (mostly cell walls), making them more suitable for cellulosic ethanol production. In this paper, we report an approach to improving saccharification of sugarcane straw by RNAi silencing of the recently discovered BAHD01 gene responsible for feruloylation of grass cell walls. Results: We identified six BAHD genes in the sugarcane genome (SacBAHDs) and generated five lines with substantially decreased SacBAHD01 expression. To find optimal conditions for determining saccharification of sugarcane straw, we tried multiple combinations of solvent and temperature pretreatment conditions, devising a predictive model for finding their effects on glucose release. Under optimal conditions, demonstrated by Organosolv pretreatment using 30% ethanol for 240 min, transgenic lines showed increases in saccharification efficiency of up to 24%. The three lines with improved saccharification efficiency had lower cell-wall ferulate content but unchanged monosaccharide and lignin compositions. Conclusions: The silencing of SacBAHD01 gene and subsequent decrease of cell-wall ferulate contents indicate a promising novel biotechnological approach for improving the suitability of sugarcane residues for cellulosic ethanol productio... Mostrar Tudo |
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Palavras-Chave: |
Cell-wall acylation; Lignocellulosic feedstock. |
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Thesaurus Nal: |
Biofuels; Biomass; Sugarcane. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198722/1/Silencing-of-a-BAHD-acyltransferase.pdf
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Marc: |
LEADER 02739naa a2200349 a 4500
001 2109978
005 2019-11-18
008 2019 bl uuuu u00u1 u #d
100 1 $aSOUZA, W. R. de
245 $aSilencing of a BAHD acyltransferase in sugarcane increases biomass digestibility.$h[electronic resource]
260 $c2019
520 $aBackground: Sugarcane (Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal candidate for improving composition of its residues (mostly cell walls), making them more suitable for cellulosic ethanol production. In this paper, we report an approach to improving saccharification of sugarcane straw by RNAi silencing of the recently discovered BAHD01 gene responsible for feruloylation of grass cell walls. Results: We identified six BAHD genes in the sugarcane genome (SacBAHDs) and generated five lines with substantially decreased SacBAHD01 expression. To find optimal conditions for determining saccharification of sugarcane straw, we tried multiple combinations of solvent and temperature pretreatment conditions, devising a predictive model for finding their effects on glucose release. Under optimal conditions, demonstrated by Organosolv pretreatment using 30% ethanol for 240 min, transgenic lines showed increases in saccharification efficiency of up to 24%. The three lines with improved saccharification efficiency had lower cell-wall ferulate content but unchanged monosaccharide and lignin compositions. Conclusions: The silencing of SacBAHD01 gene and subsequent decrease of cell-wall ferulate contents indicate a promising novel biotechnological approach for improving the suitability of sugarcane residues for cellulosic ethanol production. In addition, the Organosolv pretreatment of the genetically modified biomass and the optimal conditions for the enzymatic hydrolysis presented here might be incorporated in the sugarcane industry for bioethanol production. Keywords: Sugarcane,
650 $aBiofuels
650 $aBiomass
650 $aSugarcane
653 $aCell-wall acylation
653 $aLignocellulosic feedstock
700 1 $aPACHECO, T. F.
700 1 $aDUARTE, K. E.
700 1 $aSAMPAIO, B. L.
700 1 $aOLIVEIRA, P. A. de
700 1 $aMARTINS, P. M.
700 1 $aSANTIAGO, T. R.
700 1 $aFORMIGHIERI, E. F.
700 1 $aVINECKY, F.
700 1 $aRIBEIRO, A. P.
700 1 $aDIAS, B. B. A.
700 1 $aKOBAYASHI, A. K.
700 1 $aMITCHELL, R. A. C.
700 1 $aRODRIGUES, D. de S.
700 1 $aMOLINARI, H. B. C.
773 $tBiotechnology for Biofuels$gv. 12, n. 111, p. 1-14, 2019.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Solos. Para informações adicionais entre em contato com cnps.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
31/01/2018 |
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Data da última atualização: |
11/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 5 |
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Autoria: |
ARROUAYS, D.; LEENAARS, J. G. B.; RICHER-DE-FORGES, A. C.; ADHIKARI, K.; BALLABIO, C.; GREVE, M.; GRUNDY, M.; GUERRERO, E.; HEMPEL, J.; HENGL, T.; HEUVELINK, G.; BATJES, N.; CARVALHO, E.; HARTEMINK, A.; HEWITT, A.; HONG, S.-Y.; KRASILNIKOV, P.; LAGACHERIE, P.; LELYK, G.; LIBOHOVA, Z.; LILLY, A.; MCBRATNEY, A.; MCKENZIE, N.; VASQUES, G. de M. |
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Afiliação: |
DOMINIQUE ARROUAYS, INRA; JOHAN G. B. LEENAARS, ISRIC; ANNE C. RICHER-DE-FORGES, INRA; KABINDRA ADHIKARI, AARHUS UNIVERSITY, TJELE, DENMARK; CRISTIANO BALLABIO, EUROPEAN COMMISSION-DG JRC; MOGENS GREVE, AARHUS UNIVERSITY, TJELE, DENMAR; MIKE GRUNDY, CSIRO; ELISEO GUERRERO, INEGI, MEXICO; JON HEMPEL, USDA-NATURAL RESOURCES CONSERVATION SERVICES; TOMISLAV HENGL, ISRIC; GERARD HEUVELINK, ISRIC-WORLD SOIL INFORMATION; NIELS BATJES, ISRIC-WORLD SOIL INFORMATION; ELOI CARVALHO, ISRIC-WORLD SOIL INFORMATION; ALFRED HARTEMINK, UNIVERSITY OF WISCONSIN; ALAN HEWITT, LANDCARE RESEARCH, NEW ZEALAND; SUK-YOUNG HONG, NATIONAL ACADEMY OF AGRICULTURAL SCIENCE, REPUBLIC OF KOREA; PAVEL KRASILNIKOV, LOMONOSOV MOSCOW STATE UNIVERSITY; PHILIPPE LAGACHERIE, INRA-IRD-Supagro; GLEN LELYK, AGRICULTURE AND AGRI-FOOD CANADA; ZAMIR LIBOHOVA, USDA-NATURAL RESOURCES CONSERVATION SERVICES; ALLAN LILLY, THE JAMES HUTTON INSTITUTE, SCOTLAND; ALEX MCBRATNEY, UNIVERSITY OF SYDNEY; NEIL MCKENZIE, CSIRO; GUSTAVO DE MATTOS VASQUES, CNPS. |
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Título: |
Soil legacy data rescue via GlobalSoilMap and other international and national initiatives. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
GeoResJ, v. 14, p. 1-19, Dec. 2017. |
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DOI: |
https://doi.org/10.1016/j.grj.2017.06.001 |
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Idioma: |
Inglês |
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Conteúdo: |
Legacy soil data have been produced over 70 years in nearly all countries of the world. Unfortunately, data, information and knowledge are still currently fragmented and at risk of getting lost if they remain in a paper format. To process this legacy data into consistent, spatially explicit and continuous global soil information, data are being rescued and compiled into databases. Thousands of soil survey reports and maps have been scanned and made available online. The soil profile data reported by these data sources have been captured and compiled into databases. The total number of soil profiles rescued in the selected countries is about 800,000. Currently, data for 117, 000 profiles are compiled and harmonized according to GlobalSoilMap specifications in a world level database (WoSIS). The results presented at the country level are likely to be an underestimate. The majority of soil data is still not rescued and this effort should be pursued. The data have been used to produce soil property maps. We discuss the pro and cons of top-down and bottom-up approaches to produce such maps and we stress their complementarity. We give examples of success stories. The first global soil property maps using rescued data were produced by a top-down approach and were released at a limited resolution of 1 km in 2014, followed by an update at a resolution of 250 m in 2017. By the end of 2020, we aim to deliver the first worldwide product that fully meets the GlobalSoilMap specifications. |
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Palavras-Chave: |
GlobalSoilMap; Resgate de dados de solo. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 02681naa a2200433 a 4500
001 2086773
005 2021-11-11
008 2017 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.grj.2017.06.001$2DOI
100 1 $aARROUAYS, D.
245 $aSoil legacy data rescue via GlobalSoilMap and other international and national initiatives.$h[electronic resource]
260 $c2017
520 $aLegacy soil data have been produced over 70 years in nearly all countries of the world. Unfortunately, data, information and knowledge are still currently fragmented and at risk of getting lost if they remain in a paper format. To process this legacy data into consistent, spatially explicit and continuous global soil information, data are being rescued and compiled into databases. Thousands of soil survey reports and maps have been scanned and made available online. The soil profile data reported by these data sources have been captured and compiled into databases. The total number of soil profiles rescued in the selected countries is about 800,000. Currently, data for 117, 000 profiles are compiled and harmonized according to GlobalSoilMap specifications in a world level database (WoSIS). The results presented at the country level are likely to be an underestimate. The majority of soil data is still not rescued and this effort should be pursued. The data have been used to produce soil property maps. We discuss the pro and cons of top-down and bottom-up approaches to produce such maps and we stress their complementarity. We give examples of success stories. The first global soil property maps using rescued data were produced by a top-down approach and were released at a limited resolution of 1 km in 2014, followed by an update at a resolution of 250 m in 2017. By the end of 2020, we aim to deliver the first worldwide product that fully meets the GlobalSoilMap specifications.
653 $aGlobalSoilMap
653 $aResgate de dados de solo
700 1 $aLEENAARS, J. G. B.
700 1 $aRICHER-DE-FORGES, A. C.
700 1 $aADHIKARI, K.
700 1 $aBALLABIO, C.
700 1 $aGREVE, M.
700 1 $aGRUNDY, M.
700 1 $aGUERRERO, E.
700 1 $aHEMPEL, J.
700 1 $aHENGL, T.
700 1 $aHEUVELINK, G.
700 1 $aBATJES, N.
700 1 $aCARVALHO, E.
700 1 $aHARTEMINK, A.
700 1 $aHEWITT, A.
700 1 $aHONG, S.-Y.
700 1 $aKRASILNIKOV, P.
700 1 $aLAGACHERIE, P.
700 1 $aLELYK, G.
700 1 $aLIBOHOVA, Z.
700 1 $aLILLY, A.
700 1 $aMCBRATNEY, A.
700 1 $aMCKENZIE, N.
700 1 $aVASQUES, G. de M.
773 $tGeoResJ$gv. 14, p. 1-19, Dec. 2017.
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