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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
30/10/2015 |
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Data da última atualização: |
30/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VIEIRA, L. de J.; SANTANA, J. R. F. de; ALVES, A. A. C.; LEDO, C. A. da S.; SOUZA, F. V. D. |
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Afiliação: |
LÍVIA DE JESUS VIEIRA, UEFS; JOSÉ RANIERE FERREIRA DE SANTANA, UEFS; ALFREDO AUGUSTO CUNHA ALVES, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
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Título: |
Use of aniline blue stain to observing pollen tubes development in different Manihot Mill. species. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
African Journal of Agricultural Research, v. 10, n. 15, p. 1805-1809, 9 April, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
The lack of a reliable test to evaluate pollen viability in species of the genus Manihot is one of the primary limitations for cassava genetic improvement. Thus, the objective of the present study was to apply aniline blue stain to observing pollen tubes development in different Manihot Mill. species, in order to estimate the crossability, pollen germination time, pollen tube size and pollen longevity. Pollen grains from 2 accessions of the cultivated species M. esculenta spp. esculenta and 4 accessions of 3 wild species (M. esculenta ssp. flabellifolia (Pohl) Cif, M. anomala Pohl, and M. irwinii D. J. Roger and Appan) were used. To visualize the pollen tubes, pollinated stigmas was deposited on a glass slide with 3 drops of aniline blue stain and transferred to an optical fluorescence microscope, where they were visualized and photographed using the 10x ocular lens. The pollen crossability rate was estimated by the ercentage of germinated pollen, with well-developed pollen tubes in relation to the total number of pollen grains counted per stigma. The experimental design was entirely random with 3 repetitions, each repetition consisting of 100 pollen grains. In addition to the estimation of crossability, the germination time after pollination, pollen tube size, and pollen longevity were also determined to the accession FLA 029V-01. Significant difference was observed between the crossings. Pollen tubes developed after 45 min of contact between the pollen and the stigma. The average pollen tube size 24 h after pollination was 2.9 mm. The studies examining pollen longevity showed that germination did not occur when the pollen grains were evaluated 24 h after anthesis. Thus, the use of aniline blue stain can be considered an effective method for observing pollen tubes development and studying the pollen morphology, longevity, viability and Manihot crossability. MenosThe lack of a reliable test to evaluate pollen viability in species of the genus Manihot is one of the primary limitations for cassava genetic improvement. Thus, the objective of the present study was to apply aniline blue stain to observing pollen tubes development in different Manihot Mill. species, in order to estimate the crossability, pollen germination time, pollen tube size and pollen longevity. Pollen grains from 2 accessions of the cultivated species M. esculenta spp. esculenta and 4 accessions of 3 wild species (M. esculenta ssp. flabellifolia (Pohl) Cif, M. anomala Pohl, and M. irwinii D. J. Roger and Appan) were used. To visualize the pollen tubes, pollinated stigmas was deposited on a glass slide with 3 drops of aniline blue stain and transferred to an optical fluorescence microscope, where they were visualized and photographed using the 10x ocular lens. The pollen crossability rate was estimated by the ercentage of germinated pollen, with well-developed pollen tubes in relation to the total number of pollen grains counted per stigma. The experimental design was entirely random with 3 repetitions, each repetition consisting of 100 pollen grains. In addition to the estimation of crossability, the germination time after pollination, pollen tube size, and pollen longevity were also determined to the accession FLA 029V-01. Significant difference was observed between the crossings. Pollen tubes developed after 45 min of contact between the pollen and the stigma. The ... Mostrar Tudo |
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Thesagro: |
Mandioca; Melhoramento vegetal; Recurso genético. |
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Thesaurus Nal: |
Breeding methods; Cassava; Genetic resources. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02645naa a2200241 a 4500
001 2027633
005 2023-05-30
008 2015 bl uuuu u00u1 u #d
100 1 $aVIEIRA, L. de J.
245 $aUse of aniline blue stain to observing pollen tubes development in different Manihot Mill. species.$h[electronic resource]
260 $c2015
520 $aThe lack of a reliable test to evaluate pollen viability in species of the genus Manihot is one of the primary limitations for cassava genetic improvement. Thus, the objective of the present study was to apply aniline blue stain to observing pollen tubes development in different Manihot Mill. species, in order to estimate the crossability, pollen germination time, pollen tube size and pollen longevity. Pollen grains from 2 accessions of the cultivated species M. esculenta spp. esculenta and 4 accessions of 3 wild species (M. esculenta ssp. flabellifolia (Pohl) Cif, M. anomala Pohl, and M. irwinii D. J. Roger and Appan) were used. To visualize the pollen tubes, pollinated stigmas was deposited on a glass slide with 3 drops of aniline blue stain and transferred to an optical fluorescence microscope, where they were visualized and photographed using the 10x ocular lens. The pollen crossability rate was estimated by the ercentage of germinated pollen, with well-developed pollen tubes in relation to the total number of pollen grains counted per stigma. The experimental design was entirely random with 3 repetitions, each repetition consisting of 100 pollen grains. In addition to the estimation of crossability, the germination time after pollination, pollen tube size, and pollen longevity were also determined to the accession FLA 029V-01. Significant difference was observed between the crossings. Pollen tubes developed after 45 min of contact between the pollen and the stigma. The average pollen tube size 24 h after pollination was 2.9 mm. The studies examining pollen longevity showed that germination did not occur when the pollen grains were evaluated 24 h after anthesis. Thus, the use of aniline blue stain can be considered an effective method for observing pollen tubes development and studying the pollen morphology, longevity, viability and Manihot crossability.
650 $aBreeding methods
650 $aCassava
650 $aGenetic resources
650 $aMandioca
650 $aMelhoramento vegetal
650 $aRecurso genético
700 1 $aSANTANA, J. R. F. de
700 1 $aALVES, A. A. C.
700 1 $aLEDO, C. A. da S.
700 1 $aSOUZA, F. V. D.
773 $tAfrican Journal of Agricultural Research$gv. 10, n. 15, p. 1805-1809, 9 April, 2015.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 49 | |
| 41. |  | SILVEIRA, D. G.; AMORIM, E. P.; JESUS, O. N. de; SOUZA, F. V. D.; PESTANA, K. N.; SANTOS, V. J. dos; SANTANA, J. R. F. de. Variabilidade genética de populações naturais de caroá por meio de marcadores RAPD. Pesquisa Agropecuária Brasileira, Brasília, v.44, n. 3, p. 283-290, mar. 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Unidades Centrais. |
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| 42. | | MORAIS-LINO, L. S.; SEREJO, J. A. dos S.; AMORIM, E. P.; SANTANA, J. R. F. de; PASQUAL, M.; SILVA, S. de O. e. Somatic embryogenesis, cell suspension, and genetic stability of banana cultivars. In Vitro Cellular & Developmental Biology - Plant, November, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 43. | | MORAIS-LINO, L. S.; AMORIM, V. B. de O.; AMORIM, E. P.; SILVA, S. de O. e; SANTOS-SEREJO, J. A. dA; SANTANA, J. R. F. de. Uso de microssatelites na avaliação da estabilidade genética de mudas regeneradas de suspensão celular de bananeira 'Grande Naine'. In:CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010. pdf 2017| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 44. | | VIEIRA, L. de J.; SANTANA, J. R. F. de; ALVES, A. A. C.; LEDO, C. A. da S.; SOUZA, F. V. D. Use of aniline blue stain to observing pollen tubes development in different Manihot Mill. species. African Journal of Agricultural Research, v. 10, n. 15, p. 1805-1809, 9 April, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 45. | | VIEIRA, L. de J.; PASSOS, R. R.; OLIVIERA, T. da S.; SANTANA, J. R. F. de; LEDO, C. A. da S.; ALVES, A. A. C.; SOUZA, F. V. D. Avaliação da longevidade de grãos de pólen de de Manihot esculenta spp. flabellifolia por teste colorimétrico e fluorescência. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 46. | | VIEIRA, L. de J.; OLIVEIRA, T. da S.; PASSOS, R. R.; SANTANA, J. R. F. de; LEDO, C. A. da S.; ALVES, A. A. C.; SOUZA, F. V. D. Avaliação de métodos para desidratação de grãos de pólen de M. esculenta. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 47. | | OLIVEIRA, T. da S.; VIEIRA, L. de J.; PASSOS, R. R.; SANTANA, J. R. F. de; MARTINS, M. L. L.; LEDO, C. A. da S.; ALVES, A. A. C.; SOUZA, F. V. D. Avaliação da receptividade de estigmas de Manihot esculenta spp. flabellifolia. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 48. | | MORAIS-LINO, L. S.; AMORIM, V. B. de O.; AMORIM, E. P.; SILVA, S. de O. e; SANTOS-SEREJO, J. A. dos; CRUZ, V. C. da; SANTANA, J. R. F. de. Uso de microssatelites na avaliação da estabilidade genética de mudas regeneradas de suspensão celular de bananeira 'Maçã'. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 5., 2009, Guarapari. O melhoramento e os novos cenários da agricultura: anais. Vitória: Incaper, 2009. 1 CD-ROM. pdf 2174| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 49. | | VIEIRA, L. de J.; SANTANA, J. R. F. de; VIDAL, A. M.; ALVES, A. A. C.; SOUZA, A. da S.; LEDO, C. A. da S.; SOUZA, F. V. D. Use of multivariate analysis to evaluate the effect of sucrose on in vitro cassava conservation. African Journal of Biotechnology, February, v.14, n.5, p. 419-424, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 49 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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