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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
09/11/1999 |
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Data da última atualização: |
09/11/1999 |
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Autoria: |
FITCH, M. M. M.; MANSHARDT, R. M.; GONSALVES, D.; SLIGHTOM, J. L.; SANFORD, J. C. |
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Afiliação: |
Department of Horticulture, University of Hawaii, Honolulu; Department of Agriculture, ARS; Department of Plant Pathology Cornell, University, Geneva, New York; Department of Horticultural Sciences. |
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Título: |
Virus resistant papaya plants derived from tissues bombarded with the coat protein gene of papaya ringspot virus |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Bio Tecnology, v.10, n.11, p.14466-1472, 1992 |
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Idioma: |
Inglês |
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Conteúdo: |
Papaya ringspot virus (PRV) is a serious disease of papaya (Carica papaya L.) that has only been partially controlled by conventional methods. An alternative control method is coat protein-mediated protection (CPMP) through the trans fer and expression of the PRV coat protein (Cp) gene in papaya. We report an efficient gene transfer system utilizing microprojectile-mediated transformation of 2,4-D-treated immature zygotic embryos with a plasmid construction that contains the neomycin phosphotransferase II (NPTII) and B-glucuronidase (GUS) genes flanking a PRV cp gene expression cassette. Putative transgenic R papaya plants, regenerated on kanamycin-containing medium, were assayed for GUS and PRV coat protein expression, for the presence of NPTII and PRV cp genes [with the polymerase chain reaction (PCR) and genomic blot hybridization analysis], and for PRV cp gene transcripts by Northern analysis. |
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Categoria do assunto: |
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Marc: |
LEADER 01419naa a2200169 a 4500
001 1648380
005 1999-11-09
008 1992 bl --- 0-- u #d
100 1 $aFITCH, M. M. M.
245 $aVirus resistant papaya plants derived from tissues bombarded with the coat protein gene of papaya ringspot virus
260 $c1992
520 $aPapaya ringspot virus (PRV) is a serious disease of papaya (Carica papaya L.) that has only been partially controlled by conventional methods. An alternative control method is coat protein-mediated protection (CPMP) through the trans fer and expression of the PRV coat protein (Cp) gene in papaya. We report an efficient gene transfer system utilizing microprojectile-mediated transformation of 2,4-D-treated immature zygotic embryos with a plasmid construction that contains the neomycin phosphotransferase II (NPTII) and B-glucuronidase (GUS) genes flanking a PRV cp gene expression cassette. Putative transgenic R papaya plants, regenerated on kanamycin-containing medium, were assayed for GUS and PRV coat protein expression, for the presence of NPTII and PRV cp genes [with the polymerase chain reaction (PCR) and genomic blot hybridization analysis], and for PRV cp gene transcripts by Northern analysis.
700 1 $aMANSHARDT, R. M.
700 1 $aGONSALVES, D.
700 1 $aSLIGHTOM, J. L.
700 1 $aSANFORD, J. C.
773 $tBio Tecnology$gv.10, n.11, p.14466-1472, 1992
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| 1. |  | ARAGÃO, F. J. L.; BARROS, L. M. G.; BRASILEIRO, A. C. M.; RIBEIRO, S. G.; SMITH, F. D.; SANFORD, J. C.; FARIA, J. C.; RECH, E. L. Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) co-transformed via particle bombardment. Theoretical and Applied Genetics, Berlin, v.93, n.l/2, p.142-150, l996. p. 142-150| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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