Data/hora: 25/06/2024 10:42:16

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 09/11/1999
Data da última atualização: | 09/11/1999
Autoria: | FITCH, M. M. M.; MANSHARDT, R. M.; GONSALVES, D.; SLIGHTOM, J. L.; SANFORD, J. C.
Afiliação: | Department of Horticulture, University of Hawaii, Honolulu; Department of Agriculture, ARS; Department of Plant Pathology Cornell, University, Geneva, New York; Department of Horticultural Sciences.
Título: | Virus resistant papaya plants derived from tissues bombarded with the coat protein gene of papaya ringspot virus
Ano de publicação: | 1992
Fonte/Imprenta: | Bio Tecnology, v.10, n.11, p.14466-1472, 1992
Idioma: | Inglês
Conteúdo: | Papaya ringspot virus (PRV) is a serious disease of papaya (Carica papaya L.) that has only been partially controlled by conventional methods. An alternative control method is coat protein-mediated protection (CPMP) through the trans fer and expression of the PRV coat protein (Cp) gene in papaya. We report an efficient gene transfer system utilizing microprojectile-mediated transformation of 2,4-D-treated immature zygotic embryos with a plasmid construction that contains the neomycin phosphotransferase II (NPTII) and B-glucuronidase (GUS) genes flanking a PRV cp gene expression cassette. Putative transgenic R papaya plants, regenerated on kanamycin-containing medium, were assayed for GUS and PRV coat protein expression, for the presence of NPTII and PRV cp genes [with the polymerase chain reaction (PCR) and genomic blot hybridization analysis], and for PRV cp gene transcripts by Northern analysis.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (17412-1) - ADD - SP - -- - S11580 -  - S11580 - DIS