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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
14/10/2020 |
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Data da última atualização: |
12/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CÔNSOLO, N. R. B.; SAMUELSSON, L. M.; BARBOSA, L. C. G. S.; MONARETTO, T.; MORAES, T. B.; BUARQUE, V. L. M.; HIGUERA-PADILLA, A. R.; COLNAGO, L. A.; SILVA, S. L.; REIS, M. M.; FONSECA, A. C.; ARAÚJO, C. S. S.; LEITE, B. G. S.; ROQUE, F. A.; ARAÚJO, L. F. |
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Afiliação: |
LUIZ ALBERTO COLNAGO, CNPDIA. |
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Título: |
Characterization of chicken muscle disorders through metabolomics, pathway analysis, and water relaxometry: a pilot study. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Poultry Science, v. 99, n. 11, 2020. |
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Páginas: |
6247-6257 |
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DOI: |
https://doi.org/10.1016/j.psj.2020.06.066 |
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Idioma: |
Inglês |
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Conteúdo: |
Metabolite profiles of chicken breast extracts and water mobility in breasts were studied using proton nuclear magnetic resonance (1H-NMR) spectroscopy and time-domain NMR (TD-NMR) relaxometry, respectively, using normal breast (NB), and wooden breast (WB) and white striping (WS) myopathies in broilers. One thousand eight hundred sixty broilers were raised to commercial standards, receiving the same diets that were formulated as per the different growth stages. At 49 D of age, 200 animals were slaughtered following routine commercial procedures, and at 4 h postmortem, the whole breast (pectoralis major muscle) was removed and visually inspected by an experienced meat inspector who selected NB (without myopathies) and samples with the presence of WS and WB myopathies. Fifteen breasts (5 each of NB, WS, and WB) were analyzed through TD-NMR relaxometry, and samples of approximately 20 g were taken from each breast and frozen at −80°C for metabolite profiling through 1H-NMR spectroscopy. Multivariate statistical analysis was used to evaluate the effect on water relaxometry and metabolite profile in accordance with the presence and type of myopathy in the breast. 1H-NMR data showed that the metabolite profiles in WS and WB breasts were different from each other and from NB. This pilot study shows that myopathies appear to be related to hypoxia, connective tissue deposition, lower mitochondrial function, and greater oxidative stress compared with NB. The longitudinal and transverse relaxation time of the breasts determined by TD-NMR relaxometry was shorter for NB than that for WS and WB, indicating greater water mobility in breasts affected by myopathies. 1H-NMR spectroscopy can be used to differentiate the metabolism of WS, WB, and NB, and TD-NMR has the potential to be a fast, simple, and noninvasive method to distinguish NB from WB and WS. As a practical application, the metabolomic profile as per the occurrence of breast myopathies may be used for a better understanding of these issues, which opens a gap to mitigate the incidence and severity of WS and WB. In addition, the present study brings an opportunity for the development of a new and objective tool to classify the incidence of breast myopathies through TD-NMR relaxometry. MenosMetabolite profiles of chicken breast extracts and water mobility in breasts were studied using proton nuclear magnetic resonance (1H-NMR) spectroscopy and time-domain NMR (TD-NMR) relaxometry, respectively, using normal breast (NB), and wooden breast (WB) and white striping (WS) myopathies in broilers. One thousand eight hundred sixty broilers were raised to commercial standards, receiving the same diets that were formulated as per the different growth stages. At 49 D of age, 200 animals were slaughtered following routine commercial procedures, and at 4 h postmortem, the whole breast (pectoralis major muscle) was removed and visually inspected by an experienced meat inspector who selected NB (without myopathies) and samples with the presence of WS and WB myopathies. Fifteen breasts (5 each of NB, WS, and WB) were analyzed through TD-NMR relaxometry, and samples of approximately 20 g were taken from each breast and frozen at −80°C for metabolite profiling through 1H-NMR spectroscopy. Multivariate statistical analysis was used to evaluate the effect on water relaxometry and metabolite profile in accordance with the presence and type of myopathy in the breast. 1H-NMR data showed that the metabolite profiles in WS and WB breasts were different from each other and from NB. This pilot study shows that myopathies appear to be related to hypoxia, connective tissue deposition, lower mitochondrial function, and greater oxidative stress compared with NB. The longitudinal and transvers... Mostrar Tudo |
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Palavras-Chave: |
Chicken breast myopathy; NMR spectroscopy; TD-NMR relaxometry; Water mobilization. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03360naa a2200361 a 4500
001 2125497
005 2022-08-12
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.psj.2020.06.066$2DOI
100 1 $aCÔNSOLO, N. R. B.
245 $aCharacterization of chicken muscle disorders through metabolomics, pathway analysis, and water relaxometry$ba pilot study.$h[electronic resource]
260 $c2020
300 $a6247-6257
520 $aMetabolite profiles of chicken breast extracts and water mobility in breasts were studied using proton nuclear magnetic resonance (1H-NMR) spectroscopy and time-domain NMR (TD-NMR) relaxometry, respectively, using normal breast (NB), and wooden breast (WB) and white striping (WS) myopathies in broilers. One thousand eight hundred sixty broilers were raised to commercial standards, receiving the same diets that were formulated as per the different growth stages. At 49 D of age, 200 animals were slaughtered following routine commercial procedures, and at 4 h postmortem, the whole breast (pectoralis major muscle) was removed and visually inspected by an experienced meat inspector who selected NB (without myopathies) and samples with the presence of WS and WB myopathies. Fifteen breasts (5 each of NB, WS, and WB) were analyzed through TD-NMR relaxometry, and samples of approximately 20 g were taken from each breast and frozen at −80°C for metabolite profiling through 1H-NMR spectroscopy. Multivariate statistical analysis was used to evaluate the effect on water relaxometry and metabolite profile in accordance with the presence and type of myopathy in the breast. 1H-NMR data showed that the metabolite profiles in WS and WB breasts were different from each other and from NB. This pilot study shows that myopathies appear to be related to hypoxia, connective tissue deposition, lower mitochondrial function, and greater oxidative stress compared with NB. The longitudinal and transverse relaxation time of the breasts determined by TD-NMR relaxometry was shorter for NB than that for WS and WB, indicating greater water mobility in breasts affected by myopathies. 1H-NMR spectroscopy can be used to differentiate the metabolism of WS, WB, and NB, and TD-NMR has the potential to be a fast, simple, and noninvasive method to distinguish NB from WB and WS. As a practical application, the metabolomic profile as per the occurrence of breast myopathies may be used for a better understanding of these issues, which opens a gap to mitigate the incidence and severity of WS and WB. In addition, the present study brings an opportunity for the development of a new and objective tool to classify the incidence of breast myopathies through TD-NMR relaxometry.
653 $aChicken breast myopathy
653 $aNMR spectroscopy
653 $aTD-NMR relaxometry
653 $aWater mobilization
700 1 $aSAMUELSSON, L. M.
700 1 $aBARBOSA, L. C. G. S.
700 1 $aMONARETTO, T.
700 1 $aMORAES, T. B.
700 1 $aBUARQUE, V. L. M.
700 1 $aHIGUERA-PADILLA, A. R.
700 1 $aCOLNAGO, L. A.
700 1 $aSILVA, S. L.
700 1 $aREIS, M. M.
700 1 $aFONSECA, A. C.
700 1 $aARAÚJO, C. S. S.
700 1 $aLEITE, B. G. S.
700 1 $aROQUE, F. A.
700 1 $aARAÚJO, L. F.
773 $tPoultry Science$gv. 99, n. 11, 2020.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
20/04/2011 |
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Data da última atualização: |
10/11/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
INOUE, L. A. K. A.; BOIJINK, C. de L.; RIBEIRO, P. T.; SILVA, A. M. D. DA; AFFONSO, E. G. |
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Afiliação: |
LUIS ANTONIO KIOSHI AOKI INOUE, CPAA; CHEILA DE LIMA BOIJINK, CPAA; Patrizia Teixeira Ribeiro, Centro Universitario Nilton Lins; Ana Maria Dias da Silva, INPA; Elisabeth Gusmão Affonso, INPA. |
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Título: |
Avaliação de respostas metabólicas do tambaqui exposto ao eugenol em banhos anestésicos. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Acta Amazônica, Manaus, v. 41, n. 2, p. 327-332, jun. 2011. |
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DOI: |
http://dx.doi.org/10.1590/S0044-59672011000200020 |
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Idioma: |
Português |
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Conteúdo: |
O tambaqui é a principal espécie de peixe cultivado na Amazônia Ocidental. Porém durante o processo produtivo, práticas de manejo são necessárias para o monitoramento do crescimento e estado geral da sanidade dos animais. Para isso os animais devem ser anestesiados para maior segurança no trabalho. O eugenol, componente majoritário do óleo de cravo, tem sido bastante utilizado como anestésico alternativo para peixes por ser um produto natural e de baixo custo. Entretanto, estudos tratando de respostas metabólicas em peixes tropicais expostos a diferentes anestésicos são ainda necessários. Dentro desse intuito, o presente trabalho avaliou respostas metabólicas, detectadas por meio de alterações de parâmetros sanguíneos e plasmáticos do tambaqui, exposto ao eugenol em banhos anestésicos simulados. Respostas típicas ao estresse foram detectadas devido ao manuseio imposto aos peixes durante a realização dos banhos anestésicos. O eugenol não reduziu totalmente essas reações ao estresse. Por outro lado, esse anestésico não provocou estresse adicional em exposições curtas de 15 min em concentrações próximas a 20 mg L-1. |
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Palavras-Chave: |
Physiological responses; Respostas fisiológicas. |
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Thesagro: |
Colossoma Macropomum; Hematologia; Peixe de Água Doce; Tambaqui. |
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Thesaurus NAL: |
hematology. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32989/1/v41n2a20.pdf
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Marc: |
LEADER 01982naa a2200265 a 4500
001 1886575
005 2016-11-10
008 2011 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1590/S0044-59672011000200020$2DOI
100 1 $aINOUE, L. A. K. A.
245 $aAvaliação de respostas metabólicas do tambaqui exposto ao eugenol em banhos anestésicos.
260 $c2011
520 $aO tambaqui é a principal espécie de peixe cultivado na Amazônia Ocidental. Porém durante o processo produtivo, práticas de manejo são necessárias para o monitoramento do crescimento e estado geral da sanidade dos animais. Para isso os animais devem ser anestesiados para maior segurança no trabalho. O eugenol, componente majoritário do óleo de cravo, tem sido bastante utilizado como anestésico alternativo para peixes por ser um produto natural e de baixo custo. Entretanto, estudos tratando de respostas metabólicas em peixes tropicais expostos a diferentes anestésicos são ainda necessários. Dentro desse intuito, o presente trabalho avaliou respostas metabólicas, detectadas por meio de alterações de parâmetros sanguíneos e plasmáticos do tambaqui, exposto ao eugenol em banhos anestésicos simulados. Respostas típicas ao estresse foram detectadas devido ao manuseio imposto aos peixes durante a realização dos banhos anestésicos. O eugenol não reduziu totalmente essas reações ao estresse. Por outro lado, esse anestésico não provocou estresse adicional em exposições curtas de 15 min em concentrações próximas a 20 mg L-1.
650 $ahematology
650 $aColossoma Macropomum
650 $aHematologia
650 $aPeixe de Água Doce
650 $aTambaqui
653 $aPhysiological responses
653 $aRespostas fisiológicas
700 1 $aBOIJINK, C. de L.
700 1 $aRIBEIRO, P. T.
700 1 $aSILVA, A. M. D. DA
700 1 $aAFFONSO, E. G.
773 $tActa Amazônica, Manaus$gv. 41, n. 2, p. 327-332, jun. 2011.
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Embrapa Amazônia Ocidental (CPAA) |
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