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Registro Completo |
Biblioteca(s): |
Embrapa Agropecuária Oeste. |
Data corrente: |
27/10/2021 |
Data da última atualização: |
27/10/2021 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
BIGATÃO, P. R.; TOMAZI, M.; SALTON, J. C. |
Afiliação: |
PEDRO REVEILLEAU BIGATÃO, Graduando do curso de Agronomia, bolsista PIBIC, Centro Universitário da Grande Dourados, Dourados, MS; MICHELY TOMAZI, CPAO; JULIO CESAR SALTON, CPAO. |
Título: |
Matéria orgânica do solo em função de manejos de lavoura e pecuária. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
In: JORNADA DE INICIAÇÃO À PESQUISA DA EMBRAPA, 2021, Dourados. Resumos. Brasília, DF: Embrapa, 2021. JIPE 2021 |
ISBN: |
978-65-87380-50-6 |
Idioma: |
Português |
Palavras-Chave: |
Integração lavoura pecuária; MOS. |
Thesagro: |
Matéria Orgânica; Plantio Direto; Solo. |
Categoria do assunto: |
A Sistemas de Cultivo |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227266/1/Pedro-.pdf
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Marc: |
LEADER 00650nam a2200193 a 4500 001 2135633 005 2021-10-27 008 2021 bl uuuu u00u1 u #d 020 $a978-65-87380-50-6 100 1 $aBIGATÃO, P. R. 245 $aMatéria orgânica do solo em função de manejos de lavoura e pecuária.$h[electronic resource] 260 $aIn: JORNADA DE INICIAÇÃO À PESQUISA DA EMBRAPA, 2021, Dourados. Resumos. Brasília, DF: Embrapa, 2021. JIPE 2021$c2021 650 $aMatéria Orgânica 650 $aPlantio Direto 650 $aSolo 653 $aIntegração lavoura pecuária 653 $aMOS 700 1 $aTOMAZI, M. 700 1 $aSALTON, J. C.
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Embrapa Agropecuária Oeste (CPAO) |
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Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
14/05/2004 |
Data da última atualização: |
14/05/2004 |
Autoria: |
SALLES, J. F.; SOUZA, F. A. de; VAN ELSAS, J. D. |
Título: |
Molecular method to assess the diversity of Burkholderia species in environmental. |
Ano de publicação: |
2002 |
Fonte/Imprenta: |
Applied and Environmental Microbiology, Washington, v. 68, n.4, p. 1595-1603, 2002 |
Idioma: |
Inglês |
Conteúdo: |
In spite of the importance of many members of the genus Burkholderia in the soil microbial community, no direct method to assess the diversity of this genus has been developed so far. The aim of this work was the development of soil DNA-based PCR-denaturing gradient gel electrophoresis (DGGE), a powerful tool for studying the diversity of microbial communities, for detection and analysis of the Burkholderia diversity in soil samples. Primers specific for the genus Burkholderia were developed based on the 16S rDNA gene sequence and were evaluated in PCRs performed with genomic DNAs from Burkholderia and non-Burkholderia species as the templates. The primer system used exhibited good specificity and sensitivity for the majority of established species of the genus Burkholderia. DGGE analysis of the PCR products obtained showed that there were sufficient differences in migration behavior to distinguish the majority of the 14 Burkholderia species tested. Sequence analysis of amplicons generated with soil DNA exclusively revealed sequences affiliated with sequence of Burkholderia species, demonstrating that the PCR-DGGE method is suitable for studying the diversity of this genus in natural settings. A PCR-DGGE analysis of the Burkholderia communities en two grassland plots revealed differences in diversity mainly between bulk and rhizosphere soil samples; the communities in the latter samples produced more complex patterns. |
Thesaurus NAL: |
Burkholderia. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01923naa a2200157 a 4500 001 1625774 005 2004-05-14 008 2002 bl --- 0-- u #d 100 1 $aSALLES, J. F. 245 $aMolecular method to assess the diversity of Burkholderia species in environmental. 260 $c2002 520 $aIn spite of the importance of many members of the genus Burkholderia in the soil microbial community, no direct method to assess the diversity of this genus has been developed so far. The aim of this work was the development of soil DNA-based PCR-denaturing gradient gel electrophoresis (DGGE), a powerful tool for studying the diversity of microbial communities, for detection and analysis of the Burkholderia diversity in soil samples. Primers specific for the genus Burkholderia were developed based on the 16S rDNA gene sequence and were evaluated in PCRs performed with genomic DNAs from Burkholderia and non-Burkholderia species as the templates. The primer system used exhibited good specificity and sensitivity for the majority of established species of the genus Burkholderia. DGGE analysis of the PCR products obtained showed that there were sufficient differences in migration behavior to distinguish the majority of the 14 Burkholderia species tested. Sequence analysis of amplicons generated with soil DNA exclusively revealed sequences affiliated with sequence of Burkholderia species, demonstrating that the PCR-DGGE method is suitable for studying the diversity of this genus in natural settings. A PCR-DGGE analysis of the Burkholderia communities en two grassland plots revealed differences in diversity mainly between bulk and rhizosphere soil samples; the communities in the latter samples produced more complex patterns. 650 $aBurkholderia 700 1 $aSOUZA, F. A. de 700 1 $aVAN ELSAS, J. D. 773 $tApplied and Environmental Microbiology, Washington$gv. 68, n.4, p. 1595-1603, 2002
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