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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
04/10/2011 |
Data da última atualização: |
23/09/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SALLES, H. O.; VASCONCELOS, I. M.; SANTOS, L. F. L.; OLIVEIRA, H. D.; COSTA, P. P. C.; NASCIMENTO, N. R. F.; SANTOS, C. F.; SOUSA, D. F.; JORGE, A. R. C.; MENEZES, D. B.; MONTEIRO, H. S. A.; GONDIM, D. M. F.; OLIVEIRA, J. T. A. |
Afiliação: |
HEVILA OLIVEIRA SALLES FIGUEIREDO, CNPC; Universidade Federal do Ceará (UFC), Fortaleza, CE.; Universidade Estadual do Ceará (UECe) - Fortaleza, CE.; UECe; UECe; UECe; UECe; UFC, Fortaleza, CE.; UFC; UFC; UFC; UFC; UFC. |
Título: |
Towards a better understanding of Ipomoea asarifolia toxicity: evidence of the involvement of a leaf lectin. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Toxicon, v. 58, p. 502-508, 2011. |
DOI: |
10.1016/j.toxicon.2011.08.011 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Natural intoxication of livestock by ingestion of Ipomoea asarifolia leaves has been reported to occur widely in Brazil. Previous studies carried out by our research group provided strong evidence that a lectin could be involved with the toxic properties of I. asarifolia.To reinforce this hypothesis, a lectin-enriched fraction (LEF) was isolated from I. asarifolia leaves and its toxic effects were assessed. Leaves of I. asarifolia were excised from plants growing widely in the ?eld, mechanically wounded and maintained in a chamber at 25 3 C for 72 h in the dark, under near 100% relative humidity. The leaf proteins were extracted, ammonium sulfate precipitated, chromatographed on DEAE-cellulose and Phenyl-Sepharose to produce LEF that under SDS?PAGE showed a molecular mass of 44.0 kDa and after N-terminal amino acid analysis a primary sequence composed of AGYTPVLDIGAEVLAAGEPY. The in vivo toxicity of LEF assessed by intraorbital injection in mice showed induced severe uncoordinated movements without death. LEF reduced the muscular contraction in a dose depend way and at 29.8 mg/mL (CE50) it produces 50% inhibition of contraction, suggesting that LEF blunts autonomic neurotransmission. Isolated rat kidneys were perfused with LEF and no effects on the perfusion pressure or renal vascular resistance were observed, but urinary ?ow and glomerular ?ltration rate increased. Moreover, the percentage of tubular transport of Naþ,Kþ and Cl decreased. Histological examination of the kidneys perfused with LEF exhibited little alterations. These toxic effects observed above were concomitant with the increase of LEF hemag-glutination activity, which strongly suggest that one of the toxic principles of I. asarifolia is a lectin present in its leaves. MenosAbstract: Natural intoxication of livestock by ingestion of Ipomoea asarifolia leaves has been reported to occur widely in Brazil. Previous studies carried out by our research group provided strong evidence that a lectin could be involved with the toxic properties of I. asarifolia.To reinforce this hypothesis, a lectin-enriched fraction (LEF) was isolated from I. asarifolia leaves and its toxic effects were assessed. Leaves of I. asarifolia were excised from plants growing widely in the ?eld, mechanically wounded and maintained in a chamber at 25 3 C for 72 h in the dark, under near 100% relative humidity. The leaf proteins were extracted, ammonium sulfate precipitated, chromatographed on DEAE-cellulose and Phenyl-Sepharose to produce LEF that under SDS?PAGE showed a molecular mass of 44.0 kDa and after N-terminal amino acid analysis a primary sequence composed of AGYTPVLDIGAEVLAAGEPY. The in vivo toxicity of LEF assessed by intraorbital injection in mice showed induced severe uncoordinated movements without death. LEF reduced the muscular contraction in a dose depend way and at 29.8 mg/mL (CE50) it produces 50% inhibition of contraction, suggesting that LEF blunts autonomic neurotransmission. Isolated rat kidneys were perfused with LEF and no effects on the perfusion pressure or renal vascular resistance were observed, but urinary ?ow and glomerular ?ltration rate increased. Moreover, the percentage of tubular transport of Naþ,Kþ and Cl decreased. Histological examination o... Mostrar Tudo |
Palavras-Chave: |
Ipomoea asarifolia; Lectin; Sulato de amônia. |
Thesagro: |
Erva daninha; Lectina; Planta tóxica; Salsa; Toxidez. |
Thesaurus Nal: |
toxicity. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02855naa a2200385 a 4500 001 1902176 005 2019-09-23 008 2011 bl uuuu u00u1 u #d 024 7 $a10.1016/j.toxicon.2011.08.011$2DOI 100 1 $aSALLES, H. O. 245 $aTowards a better understanding of Ipomoea asarifolia toxicity$bevidence of the involvement of a leaf lectin.$h[electronic resource] 260 $c2011 520 $aAbstract: Natural intoxication of livestock by ingestion of Ipomoea asarifolia leaves has been reported to occur widely in Brazil. Previous studies carried out by our research group provided strong evidence that a lectin could be involved with the toxic properties of I. asarifolia.To reinforce this hypothesis, a lectin-enriched fraction (LEF) was isolated from I. asarifolia leaves and its toxic effects were assessed. Leaves of I. asarifolia were excised from plants growing widely in the ?eld, mechanically wounded and maintained in a chamber at 25 3 C for 72 h in the dark, under near 100% relative humidity. The leaf proteins were extracted, ammonium sulfate precipitated, chromatographed on DEAE-cellulose and Phenyl-Sepharose to produce LEF that under SDS?PAGE showed a molecular mass of 44.0 kDa and after N-terminal amino acid analysis a primary sequence composed of AGYTPVLDIGAEVLAAGEPY. The in vivo toxicity of LEF assessed by intraorbital injection in mice showed induced severe uncoordinated movements without death. LEF reduced the muscular contraction in a dose depend way and at 29.8 mg/mL (CE50) it produces 50% inhibition of contraction, suggesting that LEF blunts autonomic neurotransmission. Isolated rat kidneys were perfused with LEF and no effects on the perfusion pressure or renal vascular resistance were observed, but urinary ?ow and glomerular ?ltration rate increased. Moreover, the percentage of tubular transport of Naþ,Kþ and Cl decreased. Histological examination of the kidneys perfused with LEF exhibited little alterations. These toxic effects observed above were concomitant with the increase of LEF hemag-glutination activity, which strongly suggest that one of the toxic principles of I. asarifolia is a lectin present in its leaves. 650 $atoxicity 650 $aErva daninha 650 $aLectina 650 $aPlanta tóxica 650 $aSalsa 650 $aToxidez 653 $aIpomoea asarifolia 653 $aLectin 653 $aSulato de amônia 700 1 $aVASCONCELOS, I. M. 700 1 $aSANTOS, L. F. L. 700 1 $aOLIVEIRA, H. D. 700 1 $aCOSTA, P. P. C. 700 1 $aNASCIMENTO, N. R. F. 700 1 $aSANTOS, C. F. 700 1 $aSOUSA, D. F. 700 1 $aJORGE, A. R. C. 700 1 $aMENEZES, D. B. 700 1 $aMONTEIRO, H. S. A. 700 1 $aGONDIM, D. M. F. 700 1 $aOLIVEIRA, J. T. A. 773 $tToxicon$gv. 58, p. 502-508, 2011.
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Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
11/09/2002 |
Data da última atualização: |
12/11/2018 |
Autoria: |
SILVA, P. S. L. e; SILVA, P. I. B. e. |
Afiliação: |
Paulo Sérgio Lima e Silva, Escola Superior de Agricultura de Mossoró - ESAM; Paulo Igor Barbosa e Silva, Escola Superior de Agricultura de Mossoró - ESAM. |
Título: |
Efeitos de épocas de aplicação de nitrogênio no rendimento de grãos do milho. |
Ano de publicação: |
2002 |
Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 37, n. 8, p. 1057-1064, ago. 2002 |
Idioma: |
Português |
Notas: |
Título em inglês: Effects of timing of nitrogen application on grain yield of maize. |
Conteúdo: |
Um estudo foi realizado em dois anos, com irrigação por aspersão, para avaliar os efeitos da época de aplicação de 120 kg/ha de N (sulfato de amônio) no rendimento de grãos do milho. Utilizou-se o delineamento de blocos ao acaso, com cinco repetições e os seguintes tratamentos: aplicação de todo o N por ocasião do plantio (1-0-0), em cobertura, aos 25 (0-1-0) e aos 45 (0-0-1) dias após o plantio, e aplicação do N de forma parcelada (0-1/3-2/3, 1/3-0-2/3, 1/3-2/3-0, 0-1/2-1/2, 1/2-0-1/2, 1/2-1/2-0, 0-2/3-1/3, 2/3-0-1/3, 2/3-1/3-0 e 1/3-1/3-1/3). O efeito de tratamentos foi independente do efeito de anos. O maior rendimento de grãos (4.960 kg/ha) foi obtido com o tratamento 0-1/3-2/3, mas todos os demais tratamentos propiciaram rendimentos comparáveis, exceto os tratamentos 1-0-0, 1/3-2/3-0 e 2/3-1/3-0, que apresentaram os menores rendimentos. |
Palavras-Chave: |
adubos. |
Thesagro: |
Adubo; Zea Mays. |
Thesaurus NAL: |
fertilizers. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/23436/1/pab1131.pdf
|
Marc: |
LEADER 01502naa a2200193 a 4500 001 1108236 005 2018-11-12 008 2002 bl uuuu u00u1 u #d 100 1 $aSILVA, P. S. L. e 245 $aEfeitos de épocas de aplicação de nitrogênio no rendimento de grãos do milho. 260 $c2002 500 $aTítulo em inglês: Effects of timing of nitrogen application on grain yield of maize. 520 $aUm estudo foi realizado em dois anos, com irrigação por aspersão, para avaliar os efeitos da época de aplicação de 120 kg/ha de N (sulfato de amônio) no rendimento de grãos do milho. Utilizou-se o delineamento de blocos ao acaso, com cinco repetições e os seguintes tratamentos: aplicação de todo o N por ocasião do plantio (1-0-0), em cobertura, aos 25 (0-1-0) e aos 45 (0-0-1) dias após o plantio, e aplicação do N de forma parcelada (0-1/3-2/3, 1/3-0-2/3, 1/3-2/3-0, 0-1/2-1/2, 1/2-0-1/2, 1/2-1/2-0, 0-2/3-1/3, 2/3-0-1/3, 2/3-1/3-0 e 1/3-1/3-1/3). O efeito de tratamentos foi independente do efeito de anos. O maior rendimento de grãos (4.960 kg/ha) foi obtido com o tratamento 0-1/3-2/3, mas todos os demais tratamentos propiciaram rendimentos comparáveis, exceto os tratamentos 1-0-0, 1/3-2/3-0 e 2/3-1/3-0, que apresentaram os menores rendimentos. 650 $afertilizers 650 $aAdubo 650 $aZea Mays 653 $aadubos 700 1 $aSILVA, P. I. B. e 773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 37, n. 8, p. 1057-1064, ago. 2002
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