Data/hora: 21/06/2024 05:51:22

Biblioteca(s): | Embrapa Agricultura Digital.
Data corrente: | 07/02/2019
Data da última atualização: | 07/01/2020
Autoria: | MORO, C. F.; GASPAR, M.; SILVA, F. R. da; PATTATHIL, S.; HAHN, M. G.; SALGADO, I.; BRAGA, M. R.
Afiliação: | CAMILA FERNANDES MORO, Unicamp; MARILIA GASPAR, Instituto de Botânica, São Paulo; FELIPE RODRIGUES DA SILVA, CNPTIA; SIVAKUMAR PATTATHIL, University of Georgia; MICHAEL G. HAHN, University of Georgia; IONE SALGADO, Instituto de Botânica, IB/Unicamp; MARCIA REGINA BRAGA, Instituto de Botânica.
Título: | S-nitrosoglutathione promotes cell wall remodelling, alters the transcriptional profile and induces root hair formation in the hairless root hair defective 6 (rhd6) mutant of Arabidopsis thaliana.
Ano de publicação: | 2017
Fonte/Imprenta: | New Phytologist, v. 213, n. 4, p. 1771-1786, Mar. 2017.
DOI: | 10.1111/nph.14309
Idioma: | Inglês
Conteúdo: | Nitric oxide (NO) exerts pleiotropic effects on plant development; however, its involvement in cell wall modification during root hair formation (RHF) has not yet been addressed. Here, mutants of Arabidopsis thaliana with altered root hair phenotypes were used to assess the involvement of S-nitrosoglutathione (GSNO), the primary NO source, in cell wall dynamics and gene expression in roots induced to form hairs. GSNO and auxin restored the root hair phenotype of the hairless root hair defective 6 (rhd6) mutant. A positive correlation was observed between increased NO production and RHF induced by auxin in rhd6 and transparent testa glabra (ttg) mutants. Deposition of an epitope within rhamnogalacturonan-I recognized by the CCRC-M2 antibody was delayed in root hair cells (trichoblasts) compared with nonhair cells (atrichoblasts). GSNO, but not auxin, restored the wild-type root glycome and transcriptome profiles in rhd6, modulating the expression of a large number of genes related to cell wall composition and metabolism, as well as those encoding ribosomal proteins, DNA and histone-modifying enzymes and proteins involved in post-translational modification. Our results demonstrate that NO plays a key role in cell wall remodelling in trichoblasts and suggest that it also participates in chromatin modification in root cells of A. thaliana.
Palavras-Chave: | Cell wall; Glycome profile; Óxido nítrico; Parede celular; Perfil de glicoma; Root hair; S-nitrosoglutathione; Transcriptoma.
Thesaurus Nal: | Arabidopsis thaliana; Nitric oxide; Transcriptome.
Categoria do assunto: | --
Registro original: | Embrapa Agricultura Digital (CNPTIA)
Exemplares: | CNPTIA - (20028-1) - UPC - AP - DD -  -  -  - DIS