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Registro Completo |
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
03/10/2016 |
Data da última atualização: |
29/12/2017 |
Autoria: |
SILVA, J.; MORAES, C. M. de; SILVA, C. L.; SALES, G. A.; KEID, L. B.; MATOS, P. C. M.; LARA, A. P. S. S.; MORAES, C. C. G. |
Afiliação: |
JACQUELINE SILVA, PPG-SAAM/UFPA; CARINA M. DE MORAES, PPG-SAAM/UFPA; CLEYZER L. SILVA, PPG-SAAM/UFPA; GUSTAVO A. SALES, PPG-SAAM/UFPA; LARA B. KEID, USP; PAULO C. M. MATOS, PPG-SAAM/UFPA; ANA P. S. S. LARA, UFPEL; CARLA C. G. MORAES, PPG-SAAM/UFPA. |
Título: |
Brucella abortus detected in cheese from the Amazon region: differentiation of a vaccine strain (B19) from the field strain in the states of Pará, Amapá and Rondônia, Brazil. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 8, p. 705-710, ago. 2016. |
Idioma: |
Inglês |
Conteúdo: |
Brucellosis is an infectious-contagious disease responsible for significant economic losses to the meat and milk supply chain, because it causes reproductive disorders in animals and is a chronic anthropozoonosis. This study was designed to detect the DNA of Brucella spp. in cheese and to differentiate between a vaccine strain (B19) and the field strain. Sixty-six samples of different cheeses which are produced and marketed in three states of the Brazilian Amazon region (Amapá [5 samples], Pará [55 samples] and Rondônia [6 samples]) were evaluated. Thirty-nine of these samples were from cheeses made from cow’s milk, and 27 were from cheeses made from buffalo milk. Four of the 66 samples were from cheeses produced in milk processing plants regulated by the Federal Inspection Service (Serviço de Inspeção Federal); nine of the samples were from cheeses produced in processing plants regulated by the State Inspection Service (Serviço de Inspeção Estadual); five of the samples were from artisanal cheeses; and the remaining 48 samples were from informally produced cheese. DNA was obtained from the samples following a DNA extraction protocol, and PCR was conducted using primers B4 and B5 to detect Brucella spp. Primers eri1 and eri2 were used to differentiate the field strain from the B19 vaccine strain. The results showed that 21.21% (14/66) of the samples were positive for Brucella spp., of which 21.43% (3/14) were positive for the B. abortus field strain, and 7.14% (1/14) were identified as harboring vaccine strain B19. These results demonstrate that it is possible to dentify Brucella spp. in cheese from the Amazon region using the PCR technique and to differentiate the B. abortus field strain from the B19 vaccine strain. MenosBrucellosis is an infectious-contagious disease responsible for significant economic losses to the meat and milk supply chain, because it causes reproductive disorders in animals and is a chronic anthropozoonosis. This study was designed to detect the DNA of Brucella spp. in cheese and to differentiate between a vaccine strain (B19) and the field strain. Sixty-six samples of different cheeses which are produced and marketed in three states of the Brazilian Amazon region (Amapá [5 samples], Pará [55 samples] and Rondônia [6 samples]) were evaluated. Thirty-nine of these samples were from cheeses made from cow’s milk, and 27 were from cheeses made from buffalo milk. Four of the 66 samples were from cheeses produced in milk processing plants regulated by the Federal Inspection Service (Serviço de Inspeção Federal); nine of the samples were from cheeses produced in processing plants regulated by the State Inspection Service (Serviço de Inspeção Estadual); five of the samples were from artisanal cheeses; and the remaining 48 samples were from informally produced cheese. DNA was obtained from the samples following a DNA extraction protocol, and PCR was conducted using primers B4 and B5 to detect Brucella spp. Primers eri1 and eri2 were used to differentiate the field strain from the B19 vaccine strain. The results showed that 21.21% (14/66) of the samples were positive for Brucella spp., of which 21.43% (3/14) were positive for the B. abortus field strain, and 7.14% (1/14) were id... Mostrar Tudo |
Palavras-Chave: |
PCR; Vaccines (B19); Vacina B19. |
Thesagro: |
Bovideo; Brucella abortus; Queijo. |
Thesaurus Nal: |
Brucella melitensis biovar Abortus; Cattle; Cheeses. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/148211/1/Brucella-abortus-detected.pdf
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Marc: |
LEADER 02713naa a2200313 a 4500 001 2053998 005 2017-12-29 008 2016 bl uuuu u00u1 u #d 100 1 $aSILVA, J. 245 $aBrucella abortus detected in cheese from the Amazon region$bdifferentiation of a vaccine strain (B19) from the field strain in the states of Pará, Amapá and Rondônia, Brazil. 260 $c2016 520 $aBrucellosis is an infectious-contagious disease responsible for significant economic losses to the meat and milk supply chain, because it causes reproductive disorders in animals and is a chronic anthropozoonosis. This study was designed to detect the DNA of Brucella spp. in cheese and to differentiate between a vaccine strain (B19) and the field strain. Sixty-six samples of different cheeses which are produced and marketed in three states of the Brazilian Amazon region (Amapá [5 samples], Pará [55 samples] and Rondônia [6 samples]) were evaluated. Thirty-nine of these samples were from cheeses made from cow’s milk, and 27 were from cheeses made from buffalo milk. Four of the 66 samples were from cheeses produced in milk processing plants regulated by the Federal Inspection Service (Serviço de Inspeção Federal); nine of the samples were from cheeses produced in processing plants regulated by the State Inspection Service (Serviço de Inspeção Estadual); five of the samples were from artisanal cheeses; and the remaining 48 samples were from informally produced cheese. DNA was obtained from the samples following a DNA extraction protocol, and PCR was conducted using primers B4 and B5 to detect Brucella spp. Primers eri1 and eri2 were used to differentiate the field strain from the B19 vaccine strain. The results showed that 21.21% (14/66) of the samples were positive for Brucella spp., of which 21.43% (3/14) were positive for the B. abortus field strain, and 7.14% (1/14) were identified as harboring vaccine strain B19. These results demonstrate that it is possible to dentify Brucella spp. in cheese from the Amazon region using the PCR technique and to differentiate the B. abortus field strain from the B19 vaccine strain. 650 $aBrucella melitensis biovar Abortus 650 $aCattle 650 $aCheeses 650 $aBovideo 650 $aBrucella abortus 650 $aQueijo 653 $aPCR 653 $aVaccines (B19) 653 $aVacina B19 700 1 $aMORAES, C. M. de 700 1 $aSILVA, C. L. 700 1 $aSALES, G. A. 700 1 $aKEID, L. B. 700 1 $aMATOS, P. C. M. 700 1 $aLARA, A. P. S. S. 700 1 $aMORAES, C. C. G. 773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 8, p. 705-710, ago. 2016.
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Embrapa Unidades Centrais (AI-SEDE) |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
24/11/2016 |
Data da última atualização: |
23/05/2022 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
AMARAL JÚNIOR, J. M. do; MORAIS, E. de; CARMO, E. S. N. do; SOUSA, M. A. P. de; SILVA, B. A. da; MARTORANO, L. G.; BERNDT, A.; SILVA, A. G. M. e. |
Afiliação: |
João Maria do AMARAL JÚNIOR, DOUTORANDO UFPA/UFRA/EMBRAPA; Eziquiel de MORAIS, DOUTORANDO UFPA/UFRA/EMBRAPA; Elder Santana Natividade do CARMO, PÓS-GRADUANDO UFRA; Marco Antônio Paula de SOUSA, DOUTORANDO UFPA/UFRA/EMBRAPA; Bruna Almeida da SILVA, DOUTORANDA UFPA/UFRA/EMBRAPA; LUCIETA GUERREIRO MARTORANO, CPATU; ALEXANDRE BERNDT, CPPSE; André Guimarães Maciel e SILVA, UFPA. |
Título: |
Enteric methane emission of female buffaloes supplemented with palm kernel cake in the Amazon biome. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON GREENHOUSE GASES IN AGRICULTURE, 2., 2016, Campo Grande, MS. Proceedings... Brasília, DF: Embrapa, 2016. |
Páginas: |
p. 53-57. |
Série: |
(Embrapa Gado de Corte. Documentos, 216). |
Idioma: |
Inglês |
Notas: |
Coordenador Roberto Giolo de Almeida. II SIGEE. |
Conteúdo: |
Thus, in face of the concern about global warming and the efficiency of diets, this study aimed to assess the levels of inclusion of palm kernel cake on enteric methane production among female buffaloes in the Amazon. |
Palavras-Chave: |
Bubalino; Emissão de gases. |
Thesagro: |
Metano. |
Thesaurus NAL: |
Amazonia. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/150581/1/p57-61.pdf
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Marc: |
LEADER 01171nam a2200277 a 4500 001 2057093 005 2022-05-23 008 2016 bl uuuu u00u1 u #d 100 1 $aAMARAL JÚNIOR, J. M. do 245 $aEnteric methane emission of female buffaloes supplemented with palm kernel cake in the Amazon biome.$h[electronic resource] 260 $aIn: INTERNATIONAL SYMPOSIUM ON GREENHOUSE GASES IN AGRICULTURE, 2., 2016, Campo Grande, MS. Proceedings... Brasília, DF: Embrapa$c2016 300 $ap. 53-57. 490 $a(Embrapa Gado de Corte. Documentos, 216). 500 $aCoordenador Roberto Giolo de Almeida. II SIGEE. 520 $aThus, in face of the concern about global warming and the efficiency of diets, this study aimed to assess the levels of inclusion of palm kernel cake on enteric methane production among female buffaloes in the Amazon. 650 $aAmazonia 650 $aMetano 653 $aBubalino 653 $aEmissão de gases 700 1 $aMORAIS, E. de 700 1 $aCARMO, E. S. N. do 700 1 $aSOUSA, M. A. P. de 700 1 $aSILVA, B. A. da 700 1 $aMARTORANO, L. G. 700 1 $aBERNDT, A. 700 1 $aSILVA, A. G. M. e.
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