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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
16/11/2017 |
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Data da última atualização: |
23/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAMOS-GONZÁLEZ, P. L.; CHABI-JESUS, C.; GUERRA-PERAZA, O.; TASSI, A. D.; KITAJIMA, E. W.; HARAKAVA, R.; SALAROLI, R. B.; ASTUA, J. de F. |
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Afiliação: |
PEDRO LUIS RAMOS-GONZÁLEZ; CAMILA CHABI-JESUS; ORLENE GUERRA-PERAZA; ALINE DANIELE TASSI; ELLIOT WATANABE KITAJIMA; RICARDO HARAKAVA; RENATO BARBOSA SALAROLI; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
Citrus leprosis virus N: a new dichorhavirus causing citrus leprosis disease. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Phytopathology, v.107, n.8, August 2017. |
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ISSN: |
0031-949X |
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DOI: |
10.1094/PHYTO-02-17-0042-R |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus leprosis (CL) is a viral disease endemic to the Western Hemisphere that produces local necrotic and chlorotic lesions on leaves, branches, and fruit and causes serious yield reduction in citrus orchards. Samples of sweet orange (Citrus × sinensis) trees showing CL symptoms were collected during a survey in noncommercial citrus areas in the southeast region of Brazil in 2013 to 2016. Transmission electron microscopy analyses of foliar lesions confirmed the presence of rod-like viral particles commonly associated with CL in the nucleus and cytoplasm of infected cells. However, every attempt to identify these particles by reverse-transcription polymerase chain reaction tests failed, even though all described primers for the detection of known CL-causing cileviruses and dichorhaviruses were used. Next-generation sequencing of total RNA extracts from three symptomatic samples revealed the genome of distinct, although highly related (>92% nucleotide sequence identity), viruses whose genetic organization is similar to that of dichorhaviruses. The genome sequence of these viruses showed <62% nucleotide sequence identity with those of orchid fleck virus and coffee ringspot virus. Globally, the deduced amino acid sequences of the open reading frames they encode share 32.7 to 63.8% identity with the proteins of the dichorhavirids. Mites collected from both the naturally infected citrus trees and those used for the transmission of one of the characterized isolates to Arabidopsis plants were anatomically recognized as Brevipalpus phoenicis sensu stricto. Molecular and biological features indicate that the identified viruses belong to a new species of CL-associated dichorhavirus, which we propose to call Citrus leprosis N dichorhavirus. Our results, while emphasizing the increasing diversity of viruses causing CL disease, lead to a reevaluation of the nomenclature of those viruses assigned to the genus Dichorhavirus. In this regard, a comprehensive discussion is presented. MenosCitrus leprosis (CL) is a viral disease endemic to the Western Hemisphere that produces local necrotic and chlorotic lesions on leaves, branches, and fruit and causes serious yield reduction in citrus orchards. Samples of sweet orange (Citrus × sinensis) trees showing CL symptoms were collected during a survey in noncommercial citrus areas in the southeast region of Brazil in 2013 to 2016. Transmission electron microscopy analyses of foliar lesions confirmed the presence of rod-like viral particles commonly associated with CL in the nucleus and cytoplasm of infected cells. However, every attempt to identify these particles by reverse-transcription polymerase chain reaction tests failed, even though all described primers for the detection of known CL-causing cileviruses and dichorhaviruses were used. Next-generation sequencing of total RNA extracts from three symptomatic samples revealed the genome of distinct, although highly related (>92% nucleotide sequence identity), viruses whose genetic organization is similar to that of dichorhaviruses. The genome sequence of these viruses showed <62% nucleotide sequence identity with those of orchid fleck virus and coffee ringspot virus. Globally, the deduced amino acid sequences of the open reading frames they encode share 32.7 to 63.8% identity with the proteins of the dichorhavirids. Mites collected from both the naturally infected citrus trees and those used for the transmission of one of the characterized isolates to Arabidopsis ... Mostrar Tudo |
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Palavras-Chave: |
Plant disease. |
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Thesagro: |
Doença de planta; Fruta cítrica. |
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Thesaurus Nal: |
Citrus. |
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Categoria do assunto: |
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Marc: |
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001 2079883
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008 2017 bl --- 0-- u #d
022 $a0031-949X
024 7 $a10.1094/PHYTO-02-17-0042-R$2DOI
100 1 $aRAMOS-GONZÁLEZ, P. L.
245 $aCitrus leprosis virus N$ba new dichorhavirus causing citrus leprosis disease.$h[electronic resource]
260 $c2017
520 $aCitrus leprosis (CL) is a viral disease endemic to the Western Hemisphere that produces local necrotic and chlorotic lesions on leaves, branches, and fruit and causes serious yield reduction in citrus orchards. Samples of sweet orange (Citrus × sinensis) trees showing CL symptoms were collected during a survey in noncommercial citrus areas in the southeast region of Brazil in 2013 to 2016. Transmission electron microscopy analyses of foliar lesions confirmed the presence of rod-like viral particles commonly associated with CL in the nucleus and cytoplasm of infected cells. However, every attempt to identify these particles by reverse-transcription polymerase chain reaction tests failed, even though all described primers for the detection of known CL-causing cileviruses and dichorhaviruses were used. Next-generation sequencing of total RNA extracts from three symptomatic samples revealed the genome of distinct, although highly related (>92% nucleotide sequence identity), viruses whose genetic organization is similar to that of dichorhaviruses. The genome sequence of these viruses showed <62% nucleotide sequence identity with those of orchid fleck virus and coffee ringspot virus. Globally, the deduced amino acid sequences of the open reading frames they encode share 32.7 to 63.8% identity with the proteins of the dichorhavirids. Mites collected from both the naturally infected citrus trees and those used for the transmission of one of the characterized isolates to Arabidopsis plants were anatomically recognized as Brevipalpus phoenicis sensu stricto. Molecular and biological features indicate that the identified viruses belong to a new species of CL-associated dichorhavirus, which we propose to call Citrus leprosis N dichorhavirus. Our results, while emphasizing the increasing diversity of viruses causing CL disease, lead to a reevaluation of the nomenclature of those viruses assigned to the genus Dichorhavirus. In this regard, a comprehensive discussion is presented.
650 $aCitrus
650 $aDoença de planta
650 $aFruta cítrica
653 $aPlant disease
700 1 $aCHABI-JESUS, C.
700 1 $aGUERRA-PERAZA, O.
700 1 $aTASSI, A. D.
700 1 $aKITAJIMA, E. W.
700 1 $aHARAKAVA, R.
700 1 $aSALAROLI, R. B.
700 1 $aASTUA, J. de F.
773 $tPhytopathology$gv.107, n.8, August 2017.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 11 | |
| 3. |  | KITAJIMA, E. W.; TASSI, A. D.; SAITO, M. S. G.; SALAROLI, R. B.; CHABI-JESUS, C.; ASTUA, J. de F. Novas espécies hospedeiras do vírus da leprose do ligustro (Ligustrum leprosis virus-LigLV). In: CONGRESSO PAULISTA DE FITOPATOLOGIA, 40., 2017, Campinas. SP: Instituro Agronômico, 2017| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|    |
| 4. | | NOVELLI, V. M.; KUBO, K. S.; FREITAS-ASTÚA, J.; MACHADO, M. A.; SALAROLI, R. B.; KITAJIMA, E. W. Natural infection of peace lily (Spatiphyllum wallisi) by a nuclear type of Brevipalpus-transmitted virus. In: INTERNATIONAL SCIENTIFIC SEMINAR ON PLANT HEALTH, 6.; LATIN AMERICAN AND CARIBBEAN SYMPOSIUM, 2., 2008, La Habana, Cuba. Acarina Biodiversity: their use, protection and conservation. Havana, [s.n.], 2008. 1 CD-ROM. (E.3.1.42)| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|  |
| 6. | | RAMOS-GONZÁLEZ, P. L.; CHABI-JESUS, C.; GUERRA-PERAZA, O.; TASSI, A. D.; KITAJIMA, E. W.; HARAKAVA, R.; SALAROLI, R. B.; ASTUA, J. de F. Citrus leprosis virus N: a new dichorhavirus causing citrus leprosis disease. Phytopathology, v.107, n.8, August 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 7. | | CALEGARIO, R. F.; LOCALI, E. C.; STACH-MACHADO, D. R.; PERON, L. A.; CASERTA, R.; SALAROLI, R. B.; ASTUA, J. de F.; MACHADO, M. A.; KITAJIMA, E. W. Polyclonal antibodies to the putative coat protein of Citrus leprosis virus C expressed in Escherichia coli: production and use in immunodiagnosis. Tropical Plant Pathology, v.38 n.3 Brasília May/June 2013. Epub May 14, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 8. | | GARITA, L. C.; TASSI, A. D.; CALEGARIO, R. F.; ASTUA, J. de F.; SALAROLI, R. B.; ROMÃO, G. O.; KITAJIMA, E. W. Experimental host range of Citrus leprosis virus C (CiLV-C). Tropical Plant Pathology, v. 39, n.1, p.043-055, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 9. | | CALEGARIO, R. F.; KITAJIMA, E. W.; LOCALI-FABRIS, E. C.; SALAROLI, R. B.; FREITAS-ASTÚA, J.; STACH- MACHADO, D. R.; CASERTA, R.; MACHADO, M. A. In situ immunocytochemistry of citrus leaves infected with Citrus leprosis virus cytoplasmic type (CiLV-C). Tropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 10. | | KITAJIMA, E. W.; KUBO, K. S.; FERREIRA, P. de T. O.; ALCÂNTARA, B. K. de; BOARI, A. J.; GOMES, R. T.; FREITAS-ÁSTUA, J.; REZENDE, J. A. M.; MORAIS, G. J. de; SALAROLI, R. B. Chlorotic spots on Clerodendrum, a disease caused by a nuclear type of Brevipalpus (Acari: Tenuipalpidae) transmitted virus. Scientia Agricola, Piracicaba, v. 6, n. 1, p. 36-49, jan/fev. 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 11. | | CÁCERES, S.; AGUIRRE, A.; COSTA, N.; DE COLL, O.; SEGNANA, L. G.; FARIÑA, N.; TASSI, A. D.; CALEGARIO, R. F.; MORAES, G. J. de; ASTUA, J. de F.; PEREIRA, J. A.; SALAROLI, R. B.; KITAJIMA, E. W. Present status of citrus leprosis in Argentina and Paraguay. Tropical Plant Pathology, v. 38, n.4, p:282-294, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 11 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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