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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
20/07/1993 |
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Data da última atualização: |
20/07/1993 |
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Autoria: |
MIYAZAKI, K.; TANAKA, Y.; SAITO, M. |
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Título: |
Effect of temperature difference between pump and column in molecular weight determination by gel permeation chromatography. |
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Ano de publicação: |
1988 |
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Fonte/Imprenta: |
Journal of Chromatography, n.454, p.357-361, 1988. |
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Idioma: |
Inglês |
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Conteúdo: |
Siome years ago, the effect of temperature on retention times in liquid chromatography was studied by several workers, and the importance of column thermostating in order to obtain accurate and reproducible retention times (tR) was emphasized. In gel permeation chromatography (GPC), however, the effect of temperature on retention time is less significant than that in any other mode of chromatography, because it is based on steric exclusion and there is little contribution to retention of adsorption or partition, which is very temperature dependent. Therefore, it was considered to be more important to measure the flow-rate accurately in order to reduce errors in molecular wiehgt calculation. The effect of column temperature on molecular weight determinations has been reported, including the influence of solvent expansion and contaction. However, there has been no systematixc study of the errors caused by the temperature difference between the solvent discharged from a pump and the solvent in a thermostated column. This paper demonstrates that the independent changes in temperature of the solvent in the pump head and in the column results in significant errors in molecular weight determinations, and these errors can be compensated for simply by taking thermal expansion into account. |
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Palavras-Chave: |
Cromatografia liquida; Peso molecular - determinacao; Temperatura - efeito; Tempo - retencao. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01905naa a2200193 a 4500
001 1417654
005 1993-07-20
008 1988 bl --- 0-- u #d
100 1 $aMIYAZAKI, K.
245 $aEffect of temperature difference between pump and column in molecular weight determination by gel permeation chromatography.
260 $c1988
520 $aSiome years ago, the effect of temperature on retention times in liquid chromatography was studied by several workers, and the importance of column thermostating in order to obtain accurate and reproducible retention times (tR) was emphasized. In gel permeation chromatography (GPC), however, the effect of temperature on retention time is less significant than that in any other mode of chromatography, because it is based on steric exclusion and there is little contribution to retention of adsorption or partition, which is very temperature dependent. Therefore, it was considered to be more important to measure the flow-rate accurately in order to reduce errors in molecular wiehgt calculation. The effect of column temperature on molecular weight determinations has been reported, including the influence of solvent expansion and contaction. However, there has been no systematixc study of the errors caused by the temperature difference between the solvent discharged from a pump and the solvent in a thermostated column. This paper demonstrates that the independent changes in temperature of the solvent in the pump head and in the column results in significant errors in molecular weight determinations, and these errors can be compensated for simply by taking thermal expansion into account.
653 $aCromatografia liquida
653 $aPeso molecular - determinacao
653 $aTemperatura - efeito
653 $aTempo - retencao
700 1 $aTANAKA, Y.
700 1 $aSAITO, M.
773 $tJournal of Chromatography$gn.454, p.357-361, 1988.
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Embrapa Agroindústria Tropical (CNPAT) |
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| 38. |  | SAITO, M. L.; FERRAZ, J. M. G.; SCRAMIN, S.; SANTOS, C. V.; ROMEIRO, P. R. S. Compostos secundários de plantas para o controle de insetos herbívoros: ensaio com Spodoptera frugiperda. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 16.; ENCONTRO NACIONAL DE FITOSSANITARISTAS, 7., 1997, Salvador. Resumos. Salvador: SEB / EMBRAPA-CNPMF, 1997. p.370| Biblioteca(s): Embrapa Meio Ambiente. |
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