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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
12/09/2016 |
Data da última atualização: |
13/04/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SA, F. P. de; LEDO, A. da S.; AMORIM, J. A. E.; MUNIZ, A. V. C. da S.; PASQUAL, M. |
Afiliação: |
FRANCIELEN PAOLA DE SA; ANA DA SILVA LEDO, CPATC; JULIE ANNE ESPINDOLA AMORIM; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC; MOACIR PASCOAL. |
Título: |
In vitro propagation and acclimatization of genipapo accessions. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Ciência e Agrotecnologia, Lavras, MG, v. 40, n. 2, p. 155-163, mar./abr. 2016. |
Idioma: |
Inglês |
Conteúdo: |
Genipapo is a species with an intermediate seed storage behavior, and its germination is slow and asynchronous; therefore the tissue culture is an alternative strategy for the propagation in large-scale. This study aimed to evaluate three genipapo accessions at different micropropagation stages (adventitious shoot induction, in vitro rooting and acclimatization). Genipapo nodal segments from the Sabinópolis (AS), Cruz das Almas (CZA) and Núcleo Bandeirante (NB) accessions were used and inoculated in culture media with 0 and 1.0 mg L-1 6-benzylaminopurine (BAP). Different indole-3-butyric acid (IBA) concentrations were tested for in vitro rooting. Subsequently, the effect of two substrates on the seedling development of three genipapo accessions was assessed. The molecular characterization of accessions was performed using 15 inter-simple sequence repeat (ISSR) primers, and genotype clustering was performed based on genetic dissimilarity using the Unweighted Pair-Group Method with Arithmetic Mean (UPGMA). Culture media supplementation with 1.0 mg L-1 BAP favors the development of adventitious shoots and promotes a higher callogenesis percentage, leaf number and feasibility at 90 days of in vitro culture. The immersion of the basal portion of shoots for 20 seconds in all IBA solutions induces the rhizogenesis in the genipapo accessions at 90 days of in vitro culture. The substrates consisting of washed sand + dried coconut shell powder, at a 1:1 ratio by volume, and Topstrato HT® may be recommended for genipapo acclimatization. The NB accession is genetically different from the CZA and AS populations and shows superiority for most of the variables analyzed. MenosGenipapo is a species with an intermediate seed storage behavior, and its germination is slow and asynchronous; therefore the tissue culture is an alternative strategy for the propagation in large-scale. This study aimed to evaluate three genipapo accessions at different micropropagation stages (adventitious shoot induction, in vitro rooting and acclimatization). Genipapo nodal segments from the Sabinópolis (AS), Cruz das Almas (CZA) and Núcleo Bandeirante (NB) accessions were used and inoculated in culture media with 0 and 1.0 mg L-1 6-benzylaminopurine (BAP). Different indole-3-butyric acid (IBA) concentrations were tested for in vitro rooting. Subsequently, the effect of two substrates on the seedling development of three genipapo accessions was assessed. The molecular characterization of accessions was performed using 15 inter-simple sequence repeat (ISSR) primers, and genotype clustering was performed based on genetic dissimilarity using the Unweighted Pair-Group Method with Arithmetic Mean (UPGMA). Culture media supplementation with 1.0 mg L-1 BAP favors the development of adventitious shoots and promotes a higher callogenesis percentage, leaf number and feasibility at 90 days of in vitro culture. The immersion of the basal portion of shoots for 20 seconds in all IBA solutions induces the rhizogenesis in the genipapo accessions at 90 days of in vitro culture. The substrates consisting of washed sand + dried coconut shell powder, at a 1:1 ratio by volume, and Topstrato ... Mostrar Tudo |
Palavras-Chave: |
Genética de planta; Genipapo. |
Thesagro: |
Fruta tropical; Jenipapo. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02322naa a2200217 a 4500 001 2052710 005 2017-04-13 008 2016 bl uuuu u00u1 u #d 100 1 $aSA, F. P. de 245 $aIn vitro propagation and acclimatization of genipapo accessions.$h[electronic resource] 260 $c2016 520 $aGenipapo is a species with an intermediate seed storage behavior, and its germination is slow and asynchronous; therefore the tissue culture is an alternative strategy for the propagation in large-scale. This study aimed to evaluate three genipapo accessions at different micropropagation stages (adventitious shoot induction, in vitro rooting and acclimatization). Genipapo nodal segments from the Sabinópolis (AS), Cruz das Almas (CZA) and Núcleo Bandeirante (NB) accessions were used and inoculated in culture media with 0 and 1.0 mg L-1 6-benzylaminopurine (BAP). Different indole-3-butyric acid (IBA) concentrations were tested for in vitro rooting. Subsequently, the effect of two substrates on the seedling development of three genipapo accessions was assessed. The molecular characterization of accessions was performed using 15 inter-simple sequence repeat (ISSR) primers, and genotype clustering was performed based on genetic dissimilarity using the Unweighted Pair-Group Method with Arithmetic Mean (UPGMA). Culture media supplementation with 1.0 mg L-1 BAP favors the development of adventitious shoots and promotes a higher callogenesis percentage, leaf number and feasibility at 90 days of in vitro culture. The immersion of the basal portion of shoots for 20 seconds in all IBA solutions induces the rhizogenesis in the genipapo accessions at 90 days of in vitro culture. The substrates consisting of washed sand + dried coconut shell powder, at a 1:1 ratio by volume, and Topstrato HT® may be recommended for genipapo acclimatization. The NB accession is genetically different from the CZA and AS populations and shows superiority for most of the variables analyzed. 650 $aFruta tropical 650 $aJenipapo 653 $aGenética de planta 653 $aGenipapo 700 1 $aLEDO, A. da S. 700 1 $aAMORIM, J. A. E. 700 1 $aMUNIZ, A. V. C. da S. 700 1 $aPASQUAL, M. 773 $tCiência e Agrotecnologia, Lavras, MG$gv. 40, n. 2, p. 155-163, mar./abr. 2016.
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Embrapa Tabuleiros Costeiros (CPATC) |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
03/08/2015 |
Data da última atualização: |
17/07/2019 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
TORMENTE, F. V.; ANTONIOLLI, L. R.; FERREIRA, W. A.; NACHTIGALL, G. R. |
Afiliação: |
LUCIMARA ROGERIA ANTONIOLLI, CNPUV; WANDERSON ARAUJO FERREIRA, CNPUV; GILMAR RIBEIRO NACHTIGALL, CNPUV. |
Título: |
Adubação nitrogenada na qualidade pós-colheita de peras "Packham's Triumph". |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
In.:ENCONTRO DE INICIAÇÃO CIENTÍFICA, 13., 2015. Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2015. |
Páginas: |
p. 35. |
Idioma: |
Português |
Palavras-Chave: |
Adubação nitrogenada; Anais; CNPUV; IC; Iniciação cientifica; Packham’s Triumph; Pêra Packham’s Triumph. |
Thesagro: |
Pêra. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/127375/1/Tormente-13IC-p35-2015.pdf
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Marc: |
LEADER 00778nam a2200241 a 4500 001 2021225 005 2019-07-17 008 2015 bl uuuu u00u1 u #d 100 1 $aTORMENTE, F. V. 245 $aAdubação nitrogenada na qualidade pós-colheita de peras "Packham's Triumph".$h[electronic resource] 260 $aIn.:ENCONTRO DE INICIAÇÃO CIENTÍFICA, 13., 2015. Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho$c2015 300 $ap. 35. 650 $aPêra 653 $aAdubação nitrogenada 653 $aAnais 653 $aCNPUV 653 $aIC 653 $aIniciação cientifica 653 $aPackham’s Triumph 653 $aPêra Packham’s Triumph 700 1 $aANTONIOLLI, L. R. 700 1 $aFERREIRA, W. A. 700 1 $aNACHTIGALL, G. R.
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