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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
11/02/2009 |
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Data da última atualização: |
25/11/2009 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SOSA-GÓMEZ, D. R.; BINNECK, E.; MARIN, S. R. R. |
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Afiliação: |
Daniel Ricardo Sosa Gómez, CNPSo; Eliseu Binneck, CNPSo; Silvana Regina Rockenbach Marin, CNPSo. |
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Título: |
New PCR method to study the intergenic spacer (IGS) region of Nomuraea rileyi (Farlow) Samson. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: ANNUAL MEETING OF THE SOCIETY FOR INVERTEBRATE PATHOLOGY, 41.; INTERNATIONAL CONFERENCE ON BACILLUS THURINGIENSIS, 9., 2008, Warwick. Abstracts... Warwick: Society Invertebrate Pathology, 2008 |
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Idioma: |
Inglês |
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Conteúdo: |
The variability in the IGS region of the rDNA gene complex has proven useful in the development of PCR primers for strain identification. However, the lack of information on this region in Nomuraea rileyi ha precluded the development of specific primers for PCR amplification. Also, due to the high variability in this region, amplification is not possible with universal primers. Thus, to obtain sequences of the IGS region a new approach was used, a combination of IGS primer with 10-mer oligonucleotides (OD11 or E04, Operon Technologies) were used. The amplification products were visualized by agarose gel electrophoresis. DNA bands were selected for comparison between IGS-10 mer primer product amplification and DNA bands produced by 10-mer amplification. When DNA products were absent in the RAPO amplification and present in the combination IGS-10 mer amplification, these products were gel-purified, ligated into the plasmid vector TOPO TA (lnvitrogen), and amplified in DH10 B Escherichia coli cells. The DNA obtained from positive clones were subjected to Sanger sequencing. The obtained sequences ranged from 681 to 1,025 bases. Comparisons among isolates obtained from the same geographic region suggest that this technique could be used to develop strain specific primers for Nomuraea rileyi. |
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Thesagro: |
Entomologia; Nomuraea Rileyi. |
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Categoria do assunto: |
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Marc: |
LEADER 01951naa a2200169 a 4500
001 1471519
005 2009-11-25
008 2008 bl --- 0-- u #d
100 1 $aSOSA-GÓMEZ, D. R.
245 $aNew PCR method to study the intergenic spacer (IGS) region of Nomuraea rileyi (Farlow) Samson.
260 $c2008
520 $aThe variability in the IGS region of the rDNA gene complex has proven useful in the development of PCR primers for strain identification. However, the lack of information on this region in Nomuraea rileyi ha precluded the development of specific primers for PCR amplification. Also, due to the high variability in this region, amplification is not possible with universal primers. Thus, to obtain sequences of the IGS region a new approach was used, a combination of IGS primer with 10-mer oligonucleotides (OD11 or E04, Operon Technologies) were used. The amplification products were visualized by agarose gel electrophoresis. DNA bands were selected for comparison between IGS-10 mer primer product amplification and DNA bands produced by 10-mer amplification. When DNA products were absent in the RAPO amplification and present in the combination IGS-10 mer amplification, these products were gel-purified, ligated into the plasmid vector TOPO TA (lnvitrogen), and amplified in DH10 B Escherichia coli cells. The DNA obtained from positive clones were subjected to Sanger sequencing. The obtained sequences ranged from 681 to 1,025 bases. Comparisons among isolates obtained from the same geographic region suggest that this technique could be used to develop strain specific primers for Nomuraea rileyi.
650 $aEntomologia
650 $aNomuraea Rileyi
700 1 $aBINNECK, E.
700 1 $aMARIN, S. R. R.
773 $tIn: ANNUAL MEETING OF THE SOCIETY FOR INVERTEBRATE PATHOLOGY, 41.; INTERNATIONAL CONFERENCE ON BACILLUS THURINGIENSIS, 9., 2008, Warwick. Abstracts... Warwick: Society Invertebrate Pathology, 2008
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 553 | |
| 144. |  | MORALES, L.; MOSCARDI, F.; SOSA-GÓMEZ, D.R. Further results with optical brighteners as enhancers to Anticarsia gemmatalis (Lepidoptera: Noctuidae) nucleopolyhedrovirus activity. In: INTERNATIONAL COLLOQUIUM ON INVERTEBRATE PATHOLOGY AND MICROBIAL CONTROL, 8.; INTERNATIONAL CONFERENCE ON BACILLUS THURINGIENSIS 6.; ANNUAL MEETING OF THE SIP, 35., 2002, Foz do Iguassu. Program and abstracts. Londrina: Embrapa Soja: Embrapa Recursos Genéticos e Biotecnologia: UEL: SIP, 2002. p. 82-83. (Embrapa Soja. Documentos, 183; Embrapa Recursos Genéticos e Biotecnologia, 73).| Biblioteca(s): Embrapa Soja. |
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| 150. | | SOSA-GOMEZ, D.R.; DELPIN, K.; MOSCARDI, F. Efeito de fungicidas utilizados para o controle do ordio, sobre o fungo entomopatogenico, Nomuraea rileyi. In: REUNIAO DE PESQUISA DA SOJA DA REGIAO CENTRAL DO BRASIL, 20., 1998, Londrina. Ata e Resumos. Londrina: EMBRAPA-CNPSo, 1998. p.223-224. (EMBRAPA-CNPSo. Documentos, 121).| Biblioteca(s): Embrapa Soja. |
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| 157. | | SOSA-GOMEZ, D.R.; DELPIN, K. E.; COSTA, F. Intraspecific variation of the velvetbean caterpillar, Anticarsia gemmatalis Hubner, geographical populations. In: CONGRESSO BRASILEIRO DE SOJA, 2.; MERCOSOJA 2002, 2002, Foz do Iguaçu. Perspectivas do agronegócio da soja: resumos. Londrina: Embrapa Soja, 2002. p. 277. (Embrapa Soja. Documentos, 181). Organizado por Odilon Ferreira Saraiva, Clara Beatriz Hoffmann-Campo.| Biblioteca(s): Embrapa Soja. |
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| 160. | | SOSA-GOMEZ, D.R.; HUMBER, R. A.; MOSCARDI, F. Entomopathogens associated with soybean/wheat production systems in Brazil and Argentina. In: INTERNATIONAL COLLOQUIUM ON INVERTEBRATE PATHOLOGY AND MICROBIAL CONTROL, 8.; INTERNATIONAL CONFERENCE ON BACILLUS THURINGIENSIS 6.; ANNUAL MEETING OF THE SIP, 35., 2002, Foz do Iguassu. Program and abstracts. Londrina: Embrapa Soja: Embrapa Recursos Genéticos e Biotecnologia: UEL: SIP, 2002. p. 75. (Embrapa Soja. Documentos, 183; Embrapa Recursos Genéticos e Biotecnologia, 73).| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 553 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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