Data/hora: 20/06/2024 22:36:34

Biblioteca(s): 	Embrapa Recursos Genéticos e Biotecnologia.	
Data corrente: 	24/08/2005	
Data da última atualização: 	24/08/2005	
Autoria: 	SA, M. F. G. de; CHRISPEELS, M. J.	
Título: 	Molecular cloning of bruchid (Zabrotes subfasciatus) alpha-amylase cDNA and interactions of the expressed enzyme with bean amylase inhibitors.	
Ano de publicação: 	1997	
Fonte/Imprenta: 	Insect Biochemistry and Molecular Biology, v. 27, n. 4, p. 271-281, 1997.	
Idioma: 	Inglês	
Conteúdo: 	Introduction; Materials and methods: Insects, RNA extraction, Northern blot analysis, Larval extraction, Reverse transcript-polymerase chain reaction and cDNA cloning, Cells plasmid, recombinant virus and insect cell transfection, Analysis of expressed Zs alfa-amylase and amylase activity detection, Protein assay, Binding ability of the inhibitor (alfaAI-2) to expressed Zs alfa-amylase; Results: Cloning of the ZsAmy cDNA, Analysis of the transcript, ZsAmy encodes and active amylase, Inhibition of Zs-amylase by bean alfa-amylase inhibitiors, at 30°C, ZsAmy forms a complex with alfaAI-2, but not with alfaAI-1. Discussion.	
Thesagro: 	Caruncho; Clonagem; Feijão; Phaseolus Vulgaris; Zabrotes Subfasciatus.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Recursos Genéticos e Biotecnologia (CENARGEN)	
Exemplares: 	CENARGEN - (25443-1) - UPC - AP - -- - 595.7005 -  -  - DIS