Data/hora: 22/06/2024 21:14:16

Biblioteca(s): 	Embrapa Agroindústria de Alimentos; Embrapa Solos.	
Data corrente: 	16/05/2011	
Data da última atualização: 	12/06/2024	
Autoria: 	SÁ, L. R. V. de; OLIVEIRA, T. C. de; SANTOS, T. F. dos; SANTOS, A. M. dos; CAMMAROTA, M. C.; OLIVEIRA, E. M. M.; FERREIRA-LEITÃO, V. S.	
Afiliação: 	LÍVIAN RIBEIRO VASCONCELOS DE SÁ, INT; TATIANE CORREA DE OLIVEIRA, CTAA; THIAGO FERREIRA DOS SANTOS, UNIG; ANDREA MATOS DOS SANTOS, CNPS; MAGALI CHRISTE CAMMAROTA, UFRJ; EDNA MARIA MORAIS OLIVEIRA, CTAA; VIRIDIANA SANTANA FERREIRA-LEITÃO, INT.	
Título: 	Hydrogenase activity monitoring in the fermentative hydrogen production using heat pretreated sludge: a useful approach to evaluate bacterial communities performance.	
Ano de publicação: 	2011	
Fonte/Imprenta: 	International Journal of Hydrogen Energy, v. 36, n. 13, p. 7543-7549, Jul. 2011.	
DOI: 	10.1016/j.ijhydene.2011.03.119	
Idioma: 	Inglês	
Conteúdo: 	The conversion of organic compounds into H2 has received increasing attention. Enrichment of inocula by heat pretreatment eliminates non-spore forming hydrogen consuming microorganisms and promotes spore germination in genus Clostridium, which is known as one of the key hydrogen producers. Useful information about metabolic pathway is provided by some intermediate metabolites, such as: acetic, propionic, butyric and formic acids. The increase of acetic/butyric acids ratio indicates H2 production in heat pretreated inoculum when compared to untreated inoculum in the same cultivation conditions. The effect of heat pretreatment on inocula and consequently on the performance of bacterial communities responsible for H2 production was monitored through the measurement of the level of hydrogenase gene expression, as well as through the content and distribution of volatile fatty acids. The acetic acid type fermentation was followed by the microorganisms presented in untreated and heat pretreated sludge. The medium containing untreated sludge presented a ratio of acetic/butyric acid of approximately 4, the same parameter was 7 when heat pretreated sludge was employed. The level of hydrogenase gene expression tripled when heat pretreated inoculum was used, indicating a higher production of H2.	
Palavras-Chave: 	Biohidrogênio; Heat pretreatment; Hidrogenase; Hydrogenase gene; Pré-tratamento de calor; Real-time RT-PCR.	
Thesagro: 	Ácido Graxo Volátil; Hidrogênio.	
Thesaurus Nal: 	biohydrogen; mixed culture; volatile fatty acids.	
Categoria do assunto: 	P Recursos Naturais, Ciências Ambientais e da Terra
X Pesquisa, Tecnologia e Engenharia	
Registro original: 	Embrapa Agroindústria de Alimentos (CTAA)	
Exemplares: 	CNPS - (15793-1) - UPC - AP - DD -  -  - 2011.00055 - DIS
CTAA - (10976-1) - UPC - AP - PP - 2011/022 -  - 2011.00324 - DIS