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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Trigo. |
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Data corrente: |
04/09/2014 |
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Data da última atualização: |
01/02/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ZHOU, G.; PEREIRA, J. F.; DELHAIZE, E.; ZHOU, M.; MAGALHAES, J. V.; RYAN, P. R. |
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Afiliação: |
UNIVERSITY OF TASMANIA; JORGE FERNANDO PEREIRA, CNPT; CSIRO PLANT INDUSTRY; UNIVERSITY OF TASMANIA; JURANDIR VIEIRA DE MAGALHAES, CNPMS; CSIRO PLANT INDUSTRY. |
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Título: |
Enhancing the aluminium tolerance of barley by expressing the citrate transporter genes SbMATE and FRD3. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Journal of Experimental Botany, London, v. 65, n. 9, p. 2381-2390, 2014. |
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DOI: |
10.1093/jxb/eru121 |
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Idioma: |
Inglês |
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Conteúdo: |
Malate and citrate efflux from root apices is a mechanism of Al3+ tolerance in many plant species. Citrate efflux is facilitated by members of the MATE (multidrug and toxic compound exudation) family localized to the plasma membrane of root cells. Barley (Hordeum vulgare) is among the most Al3+-sensitive cereal species but the small genotypic variation in tolerance that is present is correlated with citrate efflux via a MATE transporter named HvAACT1. This study used a biotechnological approach to increase the Al3+ tolerance of barley by transforming it with two MATE genes that encode citrate transporters: SbMATE is the major Al3+-tolerance gene from sorghum whereas FRD3 is involved with Fe nutrition in Arabidopsis. Independent transgenic and null T3 lines were generated for both transgenes. Lines expressing SbMATE showed Al3+-activated citrate efflux from root apices and greater tolerance to Al3+ toxicity than nulls in hydroponic and short-term soil trials. Transgenic lines expressing FRD3 exhibited similar phenotypes except citrate release from roots occurred constitutively. The Al3+ tolerance of these lines was compared with previously generated transgenic barley lines overexpressing the endogenous HvAACT1 gene and the TaALMT1 gene from wheat. Barley lines expressing TaALMT1 showed significantly greater Al3+ tolerance than all lines expressing MATE genes. This study highlights the relative efficacy of different organic anion transport proteins for increasing the Al3+ tolerance of an important crop species. MenosMalate and citrate efflux from root apices is a mechanism of Al3+ tolerance in many plant species. Citrate efflux is facilitated by members of the MATE (multidrug and toxic compound exudation) family localized to the plasma membrane of root cells. Barley (Hordeum vulgare) is among the most Al3+-sensitive cereal species but the small genotypic variation in tolerance that is present is correlated with citrate efflux via a MATE transporter named HvAACT1. This study used a biotechnological approach to increase the Al3+ tolerance of barley by transforming it with two MATE genes that encode citrate transporters: SbMATE is the major Al3+-tolerance gene from sorghum whereas FRD3 is involved with Fe nutrition in Arabidopsis. Independent transgenic and null T3 lines were generated for both transgenes. Lines expressing SbMATE showed Al3+-activated citrate efflux from root apices and greater tolerance to Al3+ toxicity than nulls in hydroponic and short-term soil trials. Transgenic lines expressing FRD3 exhibited similar phenotypes except citrate release from roots occurred constitutively. The Al3+ tolerance of these lines was compared with previously generated transgenic barley lines overexpressing the endogenous HvAACT1 gene and the TaALMT1 gene from wheat. Barley lines expressing TaALMT1 showed significantly greater Al3+ tolerance than all lines expressing MATE genes. This study highlights the relative efficacy of different organic anion transport proteins for increasing the Al3+ tole... Mostrar Tudo |
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Palavras-Chave: |
Citrato; Tolerância ao alumínio; Transgênico. |
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Thesagro: |
Cevada; Solo Ácido. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/117026/1/2014-Journal-of-Experimental-Botany-v65n9p2381.pdf
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Marc: |
LEADER 02283naa a2200253 a 4500
001 2007369
005 2015-02-01
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1093/jxb/eru121$2DOI
100 1 $aZHOU, G.
245 $aEnhancing the aluminium tolerance of barley by expressing the citrate transporter genes SbMATE and FRD3.$h[electronic resource]
260 $c2014
520 $aMalate and citrate efflux from root apices is a mechanism of Al3+ tolerance in many plant species. Citrate efflux is facilitated by members of the MATE (multidrug and toxic compound exudation) family localized to the plasma membrane of root cells. Barley (Hordeum vulgare) is among the most Al3+-sensitive cereal species but the small genotypic variation in tolerance that is present is correlated with citrate efflux via a MATE transporter named HvAACT1. This study used a biotechnological approach to increase the Al3+ tolerance of barley by transforming it with two MATE genes that encode citrate transporters: SbMATE is the major Al3+-tolerance gene from sorghum whereas FRD3 is involved with Fe nutrition in Arabidopsis. Independent transgenic and null T3 lines were generated for both transgenes. Lines expressing SbMATE showed Al3+-activated citrate efflux from root apices and greater tolerance to Al3+ toxicity than nulls in hydroponic and short-term soil trials. Transgenic lines expressing FRD3 exhibited similar phenotypes except citrate release from roots occurred constitutively. The Al3+ tolerance of these lines was compared with previously generated transgenic barley lines overexpressing the endogenous HvAACT1 gene and the TaALMT1 gene from wheat. Barley lines expressing TaALMT1 showed significantly greater Al3+ tolerance than all lines expressing MATE genes. This study highlights the relative efficacy of different organic anion transport proteins for increasing the Al3+ tolerance of an important crop species.
650 $aCevada
650 $aSolo Ácido
653 $aCitrato
653 $aTolerância ao alumínio
653 $aTransgênico
700 1 $aPEREIRA, J. F.
700 1 $aDELHAIZE, E.
700 1 $aZHOU, M.
700 1 $aMAGALHAES, J. V.
700 1 $aRYAN, P. R.
773 $tJournal of Experimental Botany, London$gv. 65, n. 9, p. 2381-2390, 2014.
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Registro original: |
Embrapa Trigo (CNPT) |
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| Registros recuperados : 5 | |
| 3. |  | PEREIRA, J. F.; ZHOU, G.; DELHAIZE, E.; RICHARDSON, T.; ZHOU, M.; RYAN, P. R. Engineering greater aluminium resistance in wheat by over-expressing TaALMT1. Annals of Botany, v. 106, n. 1, p. 205-214, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Trigo. |
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| 4. | | ZHOU, G.; PEREIRA, J. F.; DELHAIZE, E.; ZHOU, M.; MAGALHAES, J. V.; RYAN, P. R. Enhancing the aluminium tolerance of barley by expressing the citrate transporter genes SbMATE and FRD3. Journal of Experimental Botany, London, v. 65, n. 9, p. 2381-2390, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo; Embrapa Trigo. |
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| 5. | | MINELLA, E.; FERREIRA, J. R.; PEREIRA, J. F.; RYAN, P. R.; DELHAIZE, E.; DELATORRE, C. A. Improvements in acid soil tolerance of Brazilian barley will require new allelic combinations. In: INTERNATIONAL BARLEY GENETICS SYMPOSIUM, 12., 2016, Minneapolis. Abstracts... Minneapolis: University of Minnesota, 2016. Section Abiotic stresses, poster 126.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| Registros recuperados : 5 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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